Overview

  • Product name

    Anti-KCTD21 antibody [EPR12873(2)]
    See all KCTD21 primary antibodies
  • Description

    Rabbit monoclonal [EPR12873(2)] to KCTD21
  • Host species

    Rabbit
  • Tested applications

    Suitable for: Flow Cyt, WBmore details
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Recombinant fragment within Human KCTD21 aa 150 to the C-terminus (C terminal). The exact sequence is proprietary.
    Database link: Q4G0X4

  • Positive control

    • Human fetal brain lysate. Mouse and rat brain lysate. Caco-2, C6, RAW264.7, PC-12 and NIH/3T3 cell lysate. Caco-2 cells.
  • General notes

     

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab192259 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt 1/220.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

 

WB 1/1000. Predicted molecular weight: 30 kDa.

Target

Images

  • Anti-KCTD21 antibody [EPR12873(2)] (ab192259) at 1/1000 dilution + human fetal brain lysate at 10 µg

    Secondary
    Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution

    Predicted band size: 30 kDa

  • Anti-KCTD21 antibody [EPR12873(2)] (ab192259) at 1/1000 dilution + Caco-2 cell lysate at 10 µg

    Secondary
    Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 30 kDa

  • All lanes : Anti-KCTD21 antibody [EPR12873(2)] (ab192259) at 1/1000 dilution

    Lane 1 : Mouse brain lysate
    Lane 2 : Rat brain lysate
    Lane 3 : C6 cell lysate
    Lane 4 : RAW264.7 cell lysate
    Lane 5 : PC-12 cell lysate
    Lane 6 : NIH/3T3 cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 30 kDa

  • Flow Cytometry analysis of Caco-2 (Human colorectal adenocarcinoma cell line) cells labeling KCTD21 using ab192259 at a 1/220 dilution (pink). Goat anti-rabbit IgG (FITC) used as the secondary at a 1/150 dilution. Isotype control Rabbit monoclonal IgG (green). Cells were fixed in 2% paraformaldehyde.

References

ab192259 has not yet been referenced specifically in any publications.

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