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    kctd5-antibody-epr16312-c-terminal-ab194825.pdf

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Cell Biology Proteolysis / Ubiquitin Proteasome / Ubiquitin Ubiquitin E3 Enzymes RING Finger E3 Ligase
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RecombinantRabMAb

Recombinant Anti-KCTD5 antibody [EPR16312] - C-terminal (ab194825)

  • Datasheet
  • Certificate of Compliance
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Western blot - Anti-KCTD5 antibody [EPR16312] - C-terminal (ab194825)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-KCTD5 antibody [EPR16312] - C-terminal (ab194825)
  • Western blot - Anti-KCTD5 antibody [EPR16312] - C-terminal (ab194825)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-KCTD5 antibody [EPR16312] - C-terminal (ab194825)
  • Immunocytochemistry/ Immunofluorescence - Anti-KCTD5 antibody [EPR16312] - C-terminal (ab194825)
  • Flow Cytometry (Intracellular) - Anti-KCTD5 antibody [EPR16312] - C-terminal (ab194825)
  • Anti-KCTD5 antibody [EPR16312] - C-terminal (ab194825)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR16312] to KCTD5 - C-terminal
  • Suitable for: Flow Cyt (Intra), ICC/IF, IHC-P, WB
  • Reacts with: Mouse, Rat, Human

Conjugates logo Related conjugates and formulations

Carrier Free

You may also be interested in

Protein
Product image
Recombinant Human KCTD5 protein (ab137135)
Secondary
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Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
Primary
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Anti-MKP-1 antibody (ab61201)

View more associated products

Overview

  • Product name

    Anti-KCTD5 antibody [EPR16312] - C-terminal
    See all KCTD5 primary antibodies
  • Description

    Rabbit monoclonal [EPR16312] to KCTD5 - C-terminal
  • Host species

    Rabbit
  • Tested applications

    Suitable for: Flow Cyt (Intra), ICC/IF, IHC-P, WBmore details
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • HeLa, Jurkat, RAW 264.7, C6 and NIH 3T3 whole cell lysates; Human kidney and Rat liver tissues; A431 cells; Jurkat cells.
  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.2
    Preservative: 0.01% Sodium azide
    Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Monoclonal
  • Clone number

    EPR16312
  • Isotype

    IgG
  • Research areas

    • Cell Biology
    • Proteolysis / Ubiquitin
    • Proteasome / Ubiquitin
    • Ubiquitin E3 Enzymes
    • RING Finger E3 Ligase

Associated products

  • Alternative Versions

    • Anti-KCTD5 antibody [EPR16312] - BSA and Azide free (ab251193)
  • Compatible Secondaries

    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
    • Goat Anti-Rabbit IgG H&L (HRP) (ab97051)
  • Isotype control

    • Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730)
  • Positive Controls

    • HeLa whole cell lysate (ab150035)
    • HeLa whole cell lysate (ab29545)
    • Jurkat whole cell lysate (ab30128)
    • NIH/3T3 whole cell lysate (ab7179)
    • RAW 264.7 whole cell lysate (ab7187)
    • Jurkat whole cell lysate (ab7899)
  • Recombinant Protein

    • Recombinant Human KCTD5 protein (ab137135)
  • Related Products

    • Recombinant Human KCTD5 protein (ab137135)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab194825 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt (Intra)
1/70.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

ICC/IF
1/100.
IHC-P
1/50 - 1/100. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
WB
1/1000. Detects a band of approximately 26 kDa (predicted molecular weight: 26 kDa).
Notes
Flow Cyt (Intra)
1/70.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

ICC/IF
1/100.
IHC-P
1/50 - 1/100. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
WB
1/1000. Detects a band of approximately 26 kDa (predicted molecular weight: 26 kDa).

Target

  • Function

    Its interaction with CUL3 suggests that it may act as a substrate adapter in some E3 ligase complex. Does not affect the function of Kv channel Kv2.1/KCNB1, Kv1.2/KCNA2, Kv4.2/KCND2 and Kv3.4/KCNC4.
  • Sequence similarities

    Contains 1 BTB (POZ) domain.
  • Domain

    The BTB (POZ) domain is atypical and mediates the formation of a homopentamer instead of a homotetramer. Homopentamerization is due to the presence of 4 residues in the BTB (POZ) domain: Leu-56, Gly-100, Val-112 and Ala-118.
  • Cellular localization

    Cytoplasm > cytosol. Nucleus. Predominantly cytoplasmic, translocated to the nucleus upon interaction with Rep proteins.
  • Target information above from: UniProt accession Q9NXV2 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 54442 Human
    • Entrez Gene: 69259 Mouse
    • Entrez Gene: 287109 Rat
    • Omim: 611285 Human
    • SwissProt: Q9NXV2 Human
    • SwissProt: Q8VC57 Mouse
    • SwissProt: B5DEL1 Rat
    • Unigene: 61960 Human
    • Alternative names

      • BTB/POZ domain-containing protein KCTD5 antibody
      • FLJ20040 antibody
      • KCTD5 antibody
      • KCTD5_HUMAN antibody
      • Potassium channel tetramerisation domain containing 5 antibody
      see all

    Images

    • Western blot - Anti-KCTD5 antibody [EPR16312] - C-terminal (ab194825)
      Western blot - Anti-KCTD5 antibody [EPR16312] - C-terminal (ab194825)
      All lanes : Anti-KCTD5 antibody [EPR16312] - C-terminal (ab194825) at 1/1000 dilution

      Lane 1 : HeLa whole cell lysates
      Lane 2 : Jurkat whole cell lysates

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

      Predicted band size: 26 kDa

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-KCTD5 antibody [EPR16312] - C-terminal (ab194825)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-KCTD5 antibody [EPR16312] - C-terminal (ab194825)

      Immunohistochemical analysis of paraffin-embedded Human kidney tissue labeling KCTD5 with ab194825 at 1/100 dilution (3.8 μg/ml).  A Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 was used as secondary. Counterstain: Hematoxylin.
      Inset image: negative control obtained using PBS instead of ab194825.
      Note: Both cytoplasm and nuclear staining on human kidney tissue.

      Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

    • Western blot - Anti-KCTD5 antibody [EPR16312] - C-terminal (ab194825)
      Western blot - Anti-KCTD5 antibody [EPR16312] - C-terminal (ab194825)
      All lanes : Anti-KCTD5 antibody [EPR16312] - C-terminal (ab194825) at 1/1000 dilution

      Lane 1 : C6 whole cell lysates
      Lane 2 : RAW 264.7 whole cell lysates
      Lane 3 : NIH 3T3 whole cell lysates

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

      Predicted band size: 26 kDa

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-KCTD5 antibody [EPR16312] - C-terminal (ab194825)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-KCTD5 antibody [EPR16312] - C-terminal (ab194825)

      Immunohistochemical analysis of paraffin-embedded rat liver tissue labeling KCTD5 with ab194825 at 1/100 dilution (3.8 μg/ml). A Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 was used as secondary. Counterstain: Hematoxylin.
      Inset image: negative control obtained using PBS instead of ab194825.
      Note: Both cytoplasm and nuclear staining on rat liver tissue.

      Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

    • Immunocytochemistry/ Immunofluorescence - Anti-KCTD5 antibody [EPR16312] - C-terminal (ab194825)
      Immunocytochemistry/ Immunofluorescence - Anti-KCTD5 antibody [EPR16312] - C-terminal (ab194825)

      Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized A431 cells labeling KCTD5 with ab194825 at 1/100 dilution (3.8 μg/ml), followed by Goat anti-rabbit IgG (Alexa Fluor® 488) (ab150077) secondary antibody at 1/400 dilution (green). Counterstained with Dapi (blue). Both cytoplasm and nuclear staining on A431 cell line is observed.

    • Flow Cytometry (Intracellular) - Anti-KCTD5 antibody [EPR16312] - C-terminal (ab194825)
      Flow Cytometry (Intracellular) - Anti-KCTD5 antibody [EPR16312] - C-terminal (ab194825)

      Intracellular flow cytometric analysis of Jurkat cells labeling KCTD5 with ab194825 at 1/70 dilution (Red). A Goat anti rabbit IgG (FITC) at 1/150 dilution was used as secondary antibody. Cells were fixed with 2% paraformaldehyde. Cells without incubation with primary antibody and secondary antibody (Blue). Rabbit monoclonal IgG was used as isotype control (Black). 

       

       

    • Anti-KCTD5 antibody [EPR16312] - C-terminal (ab194825)
      Anti-KCTD5 antibody [EPR16312] - C-terminal (ab194825)

    Protocols

    • Flow cytometry protocols
    • Immunohistochemistry protocols
    • Immunocytochemistry & immunofluorescence protocols
    • Western blot protocols

    Click here to view the general protocols

    Datasheets and documents

    • Datasheet download

      Download

    Certificate of Compliance

    To download a Certificate of Compliance, please enter your Lot number below:

    References (0)

    Publishing research using ab194825? Please let us know so that we can cite the reference in this datasheet.

    ab194825 has not yet been referenced specifically in any publications.

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