Recombinant
RabMAb

Recombinant Anti-KCTD5 antibody [EPR16312] - C-terminal (ab194825)

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Overview

  • Product name

    Anti-KCTD5 antibody [EPR16312] - C-terminal
    See all KCTD5 primary antibodies

  • Description

    Rabbit monoclonal [EPR16312] to KCTD5 - C-terminal

  • Host species

    Rabbit

  • Tested applications

    Suitable for: Flow Cyt, ICC/IF, IHC-P, WBmore details

  • Species reactivity

    Reacts with: Mouse, Rat, Human

  • Positive control

    • HeLa, Jurkat, RAW 264.7, C6 and NIH 3T3 whole cell lysates; Human kidney and Rat liver tissues; A431 cells; Jurkat cells.

  • General notes

     

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.

  • Storage buffer

    Preservative: 0.01% Sodium azide
    Constituents: 59% PBS, 40% Glycerol, 0.05% BSA
  • Concentration information loading...

  • Purity

    Protein A purified

  • Clonality

    Monoclonal

  • Clone number

    EPR16312

  • Isotype

    IgG

  • Research areas

Applications

Our Abpromise guarantee covers the use of ab194825 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt 1/70.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

 
ICC/IF 1/100.
IHC-P 1/50 - 1/100. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
WB 1/1000. Detects a band of approximately 26 kDa (predicted molecular weight: 26 kDa).

Target

  • Function

    Its interaction with CUL3 suggests that it may act as a substrate adapter in some E3 ligase complex. Does not affect the function of Kv channel Kv2.1/KCNB1, Kv1.2/KCNA2, Kv4.2/KCND2 and Kv3.4/KCNC4.

  • Sequence similarities

    Contains 1 BTB (POZ) domain.

  • Domain

    The BTB (POZ) domain is atypical and mediates the formation of a homopentamer instead of a homotetramer. Homopentamerization is due to the presence of 4 residues in the BTB (POZ) domain: Leu-56, Gly-100, Val-112 and Ala-118.

  • Cellular localization

    Cytoplasm > cytosol. Nucleus. Predominantly cytoplasmic, translocated to the nucleus upon interaction with Rep proteins.
  • Information by UniProt

  • Database links

    • Alternative names

      • BTB/POZ domain-containing protein KCTD5 antibody
      • FLJ20040 antibody
      • KCTD5 antibody
      • KCTD5_HUMAN antibody
      • Potassium channel tetramerisation domain containing 5 antibody
      see all

    Images

    • Western blot - Anti-KCTD5 antibody [EPR16312] - C-terminal (ab194825)
      Western blot - Anti-KCTD5 antibody [EPR16312] - C-terminal (ab194825)
      All lanes : Anti-KCTD5 antibody [EPR16312] - C-terminal (ab194825) at 1/1000 dilution

      Lane 1 : HeLa whole cell lysates
      Lane 2 : Jurkat whole cell lysates

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

      Predicted band size: 26 kDa

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-KCTD5 antibody [EPR16312] - C-terminal (ab194825)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-KCTD5 antibody [EPR16312] - C-terminal (ab194825)

      Immunohistochemical analysis of paraffin-embedded Human kidney tissue labeling KCTD5 with ab194825 at 1/100 dilution (3.8 μg/ml).  A Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 was used as secondary. Counterstain: Hematoxylin.
      Inset image: negative control obtained using PBS instead of ab194825.
      Note: Both cytoplasm and nuclear staining on human kidney tissue.

      Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

    • Western blot - Anti-KCTD5 antibody [EPR16312] - C-terminal (ab194825)
      Western blot - Anti-KCTD5 antibody [EPR16312] - C-terminal (ab194825)
      All lanes : Anti-KCTD5 antibody [EPR16312] - C-terminal (ab194825) at 1/1000 dilution

      Lane 1 : C6 whole cell lysates
      Lane 2 : RAW 264.7 whole cell lysates
      Lane 3 : NIH 3T3 whole cell lysates

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

      Predicted band size: 26 kDa

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-KCTD5 antibody [EPR16312] - C-terminal (ab194825)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-KCTD5 antibody [EPR16312] - C-terminal (ab194825)

      Immunohistochemical analysis of paraffin-embedded rat liver tissue labeling KCTD5 with ab194825 at 1/100 dilution (3.8 μg/ml). A Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 was used as secondary. Counterstain: Hematoxylin.
      Inset image: negative control obtained using PBS instead of ab194825.
      Note: Both cytoplasm and nuclear staining on rat liver tissue.

      Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

    • Immunocytochemistry/ Immunofluorescence - Anti-KCTD5 antibody [EPR16312] - C-terminal (ab194825)
      Immunocytochemistry/ Immunofluorescence - Anti-KCTD5 antibody [EPR16312] - C-terminal (ab194825)

      Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized A431 cells labeling KCTD5 with ab194825 at 1/100 dilution (3.8 μg/ml), followed by Goat anti-rabbit IgG (Alexa Fluor® 488) (ab150077) secondary antibody at 1/400 dilution (green). Counterstained with Dapi (blue). Both cytoplasm and nuclear staining on A431 cell line is observed.

    • Flow Cytometry - Anti-KCTD5 antibody [EPR16312] - C-terminal (ab194825)
      Flow Cytometry - Anti-KCTD5 antibody [EPR16312] - C-terminal (ab194825)

      Flow cytometric analysis of Jurkat cells labeling KCTD5 with ab194825 at 1/70 dilution (Red). A Goat anti rabbit IgG (FITC) at 1/150 dilution was used as secondary antibody. Cells were fixed with 2% paraformaldehyde. Cells without incubation with primary antibody and secondary antibody (Blue). Rabbit monoclonal IgG was used as isotype control (Black).

    References

    ab194825 has not yet been referenced specifically in any publications.

    Publishing research using ab194825? Please let us know so that we can cite the reference in this datasheet.

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