Recombinant Anti-KCTD9 antibody [EPR13909] - BSA and Azide free (ab250270)
![Western blot - Anti-KCTD9 antibody [EPR13909] - BSA and Azide free (ab250270)](https://www.abcam.com/ps/products/250/ab250270/Images/ab250270-402594-anti-kctd9-antibody-epr13909-bsa-and-azide-free-western-blot.jpg "Western blot - Anti-KCTD9 antibody [EPR13909] - BSA and Azide free (ab250270)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR13909] to KCTD9 - BSA and Azide free
- Suitable for: ICC, WB
- Reacts with: Human
Related conjugates and formulations
Overview
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Product name
Anti-KCTD9 antibody [EPR13909] - BSA and Azide free
See all KCTD9 primary antibodies -
Description
Rabbit monoclonal [EPR13909] to KCTD9 - BSA and Azide free -
Host species
Rabbit -
Tested applications
Suitable for: ICC, WBmore details -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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General notes
ab250270 is the carrier-free version of ab180937.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
Storage buffer
pH: 7.2
Constituent: PBS -
Carrier free
Yes -
Concentration information loading... -
Purity
Affinity purified -
Clonality
Monoclonal -
Clone number
EPR13909 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Conjugation kits
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Isotype control
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Positive Controls
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab250270 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| ICC |
Use at an assay dependent concentration.
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| WB |
Use at an assay dependent concentration. Predicted molecular weight: 43 kDa.
|
| Notes |
|---|
|
ICC
Use at an assay dependent concentration. |
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WB
Use at an assay dependent concentration. Predicted molecular weight: 43 kDa. |
Target
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Relevance
KCTD9 contains a potassium channel tetramerisation domain. The N-terminal, cytoplasmic tetramerisation domain (T1) of voltage-gated potassium channels encodes molecular determinants for subfamily-specific assembly of alpha-subunits into functional tetrameric channels. The specific function of KCTD9 is unknown. -
Cellular localization
Cytoplasmic and Plasma membrane -
Database links
- Entrez Gene: 54793 Human
- SwissProt: Q7L273 Human
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Alternative names
- BTB/POZ domain containing protein KCTD9 antibody
- BTBD27 antibody
- FLJ20038 antibody
see all
Images
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Western blot - Anti-KCTD9 antibody [EPR13909] - BSA and Azide free (ab250270)All lanes : Anti-KCTD9 antibody [EPR13909] (ab180937) at 1/50000 dilution
Lane 1 : MCF-7 cell lysate
Lane 2 : HepG2 cell lysate
Lane 3 : HT-29 cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
Predicted band size: 43 kDaThis data was developed using ab180937, the same antibody clone in a different buffer formulation.
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![Immunocytochemistry - Anti-KCTD9 antibody [EPR13909] - BSA and Azide free (ab250270)](https://www.abcam.com/ps/products/250/ab250270/Images/ab250270-1-anti-kctd9-antibody-epr13909-bsa-and-azide-free-immunocytochemistry.jpg)
Immunocytochemistry - Anti-KCTD9 antibody [EPR13909] - BSA and Azide free (ab250270)This data was developed using ab180937, the same antibody clone in a different buffer formulation.Immunofluorescence analysis of MCF7 cells labeling KCTD9 using ab180937 at 1/50 dilution. A Goat anti rabbit IgG (Alexa Fluor488) at 1/200 dilution was used as secondary antibody. Cells were fixed with 4% paraformaldehyde. Counterstain: DAPI. -
![Anti-KCTD9 antibody [EPR13909] - BSA and Azide free (ab250270)](https://www.abcam.com/ps/products/250/ab250270/Images/ab250270-3-benefits-of-recombinant-antibodies.png)
Anti-KCTD9 antibody [EPR13909] - BSA and Azide free (ab250270)
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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Datasheet download
Certificate of Compliance
References (0)
ab250270 has not yet been referenced specifically in any publications.
