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    kctd9-antibody-epr13909-bsa-and-azide-free-ab250270.pdf

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Neuroscience Neurotransmission Receptors / Channels Potassium Channels
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RecombinantRabMAb

Recombinant Anti-KCTD9 antibody [EPR13909] - BSA and Azide free (ab250270)

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  • Certificate of Compliance
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Western blot - Anti-KCTD9 antibody [EPR13909] - BSA and Azide free (ab250270)
  • Immunocytochemistry - Anti-KCTD9 antibody [EPR13909] - BSA and Azide free (ab250270)
  • Anti-KCTD9 antibody [EPR13909] - BSA and Azide free (ab250270)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR13909] to KCTD9 - BSA and Azide free
  • Suitable for: ICC, WB
  • Reacts with: Human

Conjugates logo Related conjugates and formulations

Unconjugated

You may also be interested in

Primary
Product image
Anti-KCTD9 antibody [EPR13909] (ab180937)
Conjugation
Product image
Alexa Fluor® 488 Conjugation Kit (Fast) - Lightning-Link® (ab236553)

View more associated products

Overview

  • Product name

    Anti-KCTD9 antibody [EPR13909] - BSA and Azide free
    See all KCTD9 primary antibodies
  • Description

    Rabbit monoclonal [EPR13909] to KCTD9 - BSA and Azide free
  • Host species

    Rabbit
  • Tested applications

    Suitable for: ICC, WBmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • General notes

    ab250270 is the carrier-free version of ab180937.

    Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.

    This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

    Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

    This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C. Do Not Freeze.
  • Storage buffer

    pH: 7.2
    Constituent: PBS
  • Carrier free

    Yes
  • Concentration information loading...
  • Purity

    Affinity purified
  • Clonality

    Monoclonal
  • Clone number

    EPR13909
  • Isotype

    IgG
  • Research areas

    • Neuroscience
    • Neurotransmission
    • Receptors / Channels
    • Potassium Channels
    • Signal Transduction
    • Metabolism
    • Plasma Membrane
    • Channels

Associated products

  • Alternative Versions

    • Anti-KCTD9 antibody [EPR13909] (ab180937)
  • Conjugation kits

    • FITC Conjugation Kit (Fast) - Lightning-Link® (ab188285)
  • Isotype control

    • Rabbit IgG, monoclonal [EPR25A] - Isotype Control - BSA and Azide Free (ab210849)
  • Positive Controls

    • HT-29 whole cell lysate (ab3952)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab250270 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC
Use at an assay dependent concentration.
WB
Use at an assay dependent concentration. Predicted molecular weight: 43 kDa.
Notes
ICC
Use at an assay dependent concentration.
WB
Use at an assay dependent concentration. Predicted molecular weight: 43 kDa.

Target

  • Relevance

    KCTD9 contains a potassium channel tetramerisation domain. The N-terminal, cytoplasmic tetramerisation domain (T1) of voltage-gated potassium channels encodes molecular determinants for subfamily-specific assembly of alpha-subunits into functional tetrameric channels. The specific function of KCTD9 is unknown.
  • Cellular localization

    Cytoplasmic and Plasma membrane
  • Database links

    • Entrez Gene: 54793 Human
    • SwissProt: Q7L273 Human
    • Alternative names

      • BTB/POZ domain containing protein KCTD9 antibody
      • BTBD27 antibody
      • FLJ20038 antibody
      • KCTD 9 antibody
      • Potassium channel tetramerisation domain containing 9 antibody
      see all

    Images

    • Western blot - Anti-KCTD9 antibody [EPR13909] - BSA and Azide free (ab250270)
      Western blot - Anti-KCTD9 antibody [EPR13909] - BSA and Azide free (ab250270)
      All lanes : Anti-KCTD9 antibody [EPR13909] (ab180937) at 1/50000 dilution

      Lane 1 : MCF-7 cell lysate
      Lane 2 : HepG2 cell lysate
      Lane 3 : HT-29 cell lysate

      Lysates/proteins at 20 µg per lane.

      Secondary
      All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

      Predicted band size: 43 kDa



      This data was developed using ab180937, the same antibody clone in a different buffer formulation.

    • Immunocytochemistry - Anti-KCTD9 antibody [EPR13909] - BSA and Azide free (ab250270)
      Immunocytochemistry - Anti-KCTD9 antibody [EPR13909] - BSA and Azide free (ab250270)
      This data was developed using ab180937, the same antibody clone in a different buffer formulation.Immunofluorescence analysis of MCF7 cells labeling KCTD9 using ab180937 at 1/50 dilution. A Goat anti rabbit IgG (Alexa Fluor488) at 1/200 dilution was used as secondary antibody. Cells were fixed with 4% paraformaldehyde. Counterstain: DAPI.
    • Anti-KCTD9 antibody [EPR13909] - BSA and Azide free (ab250270)
      Anti-KCTD9 antibody [EPR13909] - BSA and Azide free (ab250270)

    Protocols

    To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

    Click here to view the general protocols

    Datasheets and documents

    • Datasheet download

      Download

    Certificate of Compliance

    To download a Certificate of Compliance, please enter your Lot number below:

    References (0)

    Publishing research using ab250270? Please let us know so that we can cite the reference in this datasheet.

    ab250270 has not yet been referenced specifically in any publications.

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