Overview

  • Product name

    Anti-KDEL antibody [10C3] (DyLight® 488)
    See all KDEL primary antibodies
  • Description

    Mouse monoclonal [10C3] to KDEL (DyLight® 488)
  • Host species

    Mouse
  • Conjugation

    DyLight® 488. Ex: 493nm, Em: 518nm
  • Specificity

    Recognizes proteins containing the KDEL sequence.
  • Tested applications

    Suitable for: Flow Cytmore details
  • Species reactivity

    Reacts with: Rat, Human
    Predicted to work with: Bird, Plants, Mammals
  • Immunogen

    Synthetic peptide corresponding to Rat KDEL aa 649-654.
    Sequence:

    SEKDEL

  • Positive control

    • Jurkat cells.
  • General notes

    This product was changed from ascites to tissue culture supernatant on 12th September 2018. Please note that the dilutions may need to be adjusted accordingly. If you have any questions, please do not hesitate to contact our scientific support team.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C.
  • Storage buffer

    Preservative: 0.09% Sodium azide
    Constituent: PBS
  • Concentration information loading...
  • Purity

    Protein G purified
  • Purification notes

    Purified from TCS
  • Clonality

    Monoclonal
  • Clone number

    10C3
  • Isotype

    IgG2a
  • Research areas

Associated products

Applications

Our Abpromise guarantee covers the use of ab115638 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt 1/20.

Target

  • Relevance

    The sequence Lys-Asp-Glu-Leu (KDEL) or a closely related sequence, is present at the carboxy-terminus of soluble endoplasmic reticulum (ER) resident proteins and some membrane proteins. 78 and 94 kDa glucose regulated proteins (GRP 78) and GRP 94 respectively and protein disulfide isomerase (PDI) all share the C-terminal KDEL sequence. The presence of carboxy-terminal KDEL appears to be necessary for ER retention and appears to be sufficient to reduce the secretion of proteins from the ER. This retention is reported to be mediated by a KDEL receptor.
  • Cellular localization

    Endoplasmic reticulum
  • Alternative names

    • KDEL antibody
    • Lys Asp Glu Leu antibody

Images

  • ab115638 at 10µg/ml staining KDEL in 506 Jurkat cells by Flow cytometry (shown in green).
    Grey: unstained;Yellow: isotype control.

References

This product has been referenced in:

  • Lee JH  et al. Hypoxic Preconditioning Promotes the Bioactivities of Mesenchymal Stem Cells via the HIF-1a-GRP78-Akt Axis. Int J Mol Sci 18:N/A (2017). Flow Cyt ; Human . Read more (PubMed: 28635661) »
See 1 Publication for this product

Customer reviews and Q&As

1-4 of 4 Abreviews or Q&A

Answer

Thank you for contacting us. This antibody has not been tested without permeabilization. Due to its intracellular localization, the laboratory skeptical that it would work this way.

I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

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Answer

Thank you for contacting us regarding the flow cytometry protocol for ab115638.

I do wish to apologize for the delay in getting this information to you. The protocol used by the lab is attached.

I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

Free Rabbit monoclonal antibody with any purchase of a primary antibody, while stocks last! Quote “RABMAB-XBSMG” in your next primary antibody order. For more information, visit the following link: https://www.abcam.com/index.html?pageconfig=resource&rid=15447

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Answer

Thank you for contacting us. Unofrtunately we do not have the Dylight 488 isotype control available in our catalog.


I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

Use our products? Submit an Abreview. Earn rewards!
https://www.abcam.com/abreviews

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Answer

Thank you for contacting Abcam and for your interest in ab115638.


As we discussed, I have updated the datasheet to reflect that human is a tested species guaranteed by our Abpromise. I apologize for the discrepancy and for any confusion this may have caused. Thank you for pointing out this error.


I hope this information is helpful. Please do not hesitate to contact us if you have any additional questions. I wish you the best of luck with your experiments!

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Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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