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    kdel-receptor-antibody-kr-10-ab69659.pdf

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Anti-KDEL Receptor antibody [KR-10] (ab69659)

  • Datasheet
Reviews (4) Submit a question References (8)

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Western blot - Anti-KDEL Receptor antibody [KR-10] (ab69659)

    Key features and details

    • Mouse monoclonal [KR-10] to KDEL Receptor
    • Suitable for: WB
    • Reacts with: Mouse, Rat, Sheep, Rabbit, Chicken, Hamster, Cow, Dog, Human, Pig, Xenopus laevis, Drosophila melanogaster, Monkey
    • Isotype: IgG1

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    Overview

    • Product name

      Anti-KDEL Receptor antibody [KR-10]
      See all KDEL Receptor primary antibodies
    • Description

      Mouse monoclonal [KR-10] to KDEL Receptor
    • Host species

      Mouse
    • Tested applications

      Suitable for: WBmore details
    • Species reactivity

      Reacts with: Mouse, Rat, Sheep, Rabbit, Chicken, Hamster, Cow, Dog, Human, Pig, Xenopus laevis, Drosophila melanogaster, Monkey
    • Immunogen

      Synthetic peptide corresponding to Cow KDEL Receptor aa 192-212 conjugated to keyhole limpet haemocyanin. 21 residue synthetic peptide.
      Sequence:

      CDFFYLYITKVLKGKKLSLPA


      Database link: Q99JH8
      Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI
    • General notes

      Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

      Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

      We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

      In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

      We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

      Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

      Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

    Properties

    • Form

      Liquid
    • Storage instructions

      Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
    • Storage buffer

      Preservative: 0.09% Sodium azide
      Constituents: 50% Glycerol (glycerin, glycerine), PBS
    • Concentration information loading...
    • Purity

      Protein G purified
    • Clonality

      Monoclonal
    • Clone number

      KR-10
    • Isotype

      IgG1
    • Research areas

      • Tags & Cell Markers
      • Subcellular Markers
      • Organelles
      • ER
      • Signal Transduction
      • Protein Trafficking
      • ER Proteins

    Associated products

    • Alternative Versions

      • HRP Anti-KDEL Receptor antibody [KR-10] (ab183379)
    • Compatible Secondaries

      • Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) (ab150113)
      • Goat Anti-Mouse IgG H&L (HRP) (ab205719)
      • Goat Anti-Mouse IgG H&L (DyLight® 488) preadsorbed (ab96879)
    • Conjugation kits

      • PE / R-Phycoerythrin Conjugation Kit - Lightning-Link® (ab102918)
      • APC Conjugation Kit - Lightning-Link® (ab201807)
    • Corresponding Antibody

      • HRP Anti-KDEL Receptor antibody [KR-10] (ab183379)
    • Isotype control

      • Mouse IgG1, kappa monoclonal [15-6E10A7] - Isotype Control (ab170190)
      • Mouse IgG1, Kappa Monoclonal [B11/6] - Isotype Control (ab91353)
    • Recombinant Protein

      • Recombinant Human KDEL Receptor protein (ab153142)

    Applications

    Our Abpromise guarantee covers the use of ab69659 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Application Abreviews Notes
    WB 1/1000. Predicted molecular weight: 24 kDa.

    Target

    • Relevance

      Retention of resident soluble proteins in the lumen of the endoplasmic reticulum (ER) is achieved in both yeast and animal cells by their continual retrieval from the cis-Golgi, or a pre-Golgi compartment. Sorting of these proteins is dependent on a C-terminal tetrapeptide signal, usually lys-asp-glu-leu (KDEL) in animal cells, and his-asp-glu-leu (HDEL) in S. cerevisiae. This process is mediated by a receptor that recognizes, and binds the tetrapeptide-containing protein, and returns it to the ER. In yeast, the sorting receptor encoded by a single gene, ERD2, which is a seven-transmembrane protein. Unlike yeast, several human homologs of the ERD2 gene, constituting the KDEL receptor gene family, have been described. The protein encoded by this gene was the first member of the family to be identified, and it encodes a protein structurally and functionally similar to the yeast ERD2 gene product.
    • Cellular localization

      Endoplasmic reticulum
    • Database links

      • Entrez Gene: 618184 Cow
      • Entrez Gene: 484406 Dog
      • Entrez Gene: 10945 Human
      • Entrez Gene: 68137 Mouse
      • Entrez Gene: 361577 Rat
      • Omim: 131235 Human
      • SwissProt: P33946 Cow
      • SwissProt: P24390 Human
      • SwissProt: Q99JH8 Mouse
      • SwissProt: Q569A6 Rat
      • Unigene: 515515 Human
      • Unigene: 298433 Mouse
      • Unigene: 1819 Rat
      see all
    • Alternative names

      • ER lumen protein retaining receptor 1 antibody
      • ERD 2 antibody
      • ERD2 antibody
      • ERD2.1 antibody
      • ERD21 antibody
      • HDEL antibody
      • KDEL (Lys Asp Glu Leu) endoplasmic reticulum protein retention receptor 1 antibody
      • KDEL (Lys-Asp-Glu-Leu) 72 kD endoplasmic reticulum protein retention receptor 1 antibody
      • KDEL endoplasmic reticulum protein retention receptor 1 antibody
      • KDEL R1 antibody
      • KDEL receptor 1 antibody
      • KDELR 1 antibody
      • KDELR1 antibody
      • PM 23 antibody
      • PM23 antibody
      • Putative MAPK activating protein PM23 antibody
      see all

    Images

    • Western blot - Anti-KDEL Receptor antibody [KR-10] (ab69659)
      Western blot - Anti-KDEL Receptor antibody [KR-10] (ab69659)
      Anti-KDEL Receptor antibody [KR-10] (ab69659) at 1/1000 dilution + Rat tissue lysate at 15 µg

      Secondary
      Sheep Anti-Mouse IgG: HRP for 1 hour at RT.

      Predicted band size: 24 kDa

    Protocols

    • Flow cytometry protocols
    • Western blot protocols
    • Protocol Booklet

    Click here to view the general protocols

    Datasheets and documents

    • Datasheet
  • References (8)

    Publishing research using ab69659? Please let us know so that we can cite the reference in this datasheet.

    ab69659 has been referenced in 8 publications.

    • Bartels AK  et al. KDEL Receptor 1 Contributes to Cell Surface Association of Protein Disulfide Isomerases. Cell Physiol Biochem 52:850-868 (2019). PubMed: 30958660
    • Lo Piccolo L  et al. FUS toxicity is rescued by the modulation of lncRNA hsr? expression in Drosophila melanogaster. Sci Rep 7:15660 (2017). PubMed: 29142303
    • Qu Z  et al. Label-Free Quantitative Proteomic Analysis of Differentially Expressed Membrane Proteins of Pulmonary Alveolar Macrophages Infected with Highly Pathogenic Porcine Reproductive and Respiratory Syndrome Virus and Its Attenuated Strain. Proteomics 17:N/A (2017). PubMed: 29052333
    • Aguilera-Gomez A  et al. In vivo vizualisation of mono-ADP-ribosylation by dPARP16 upon amino-acid starvation. Elife 5:N/A (2016). ICC/IF ; Drosophila melanogaster . PubMed: 27874829
    • Wells MB & Andrew DJ "Salivary gland cellular architecture in the Asian malaria vector mosquito Anopheles stephensi". Parasit Vectors 8:617 (2015). PubMed: 26627194
    • Chiang CK  et al. Endoplasmic reticulum stress implicated in the development of renal fibrosis. Mol Med 17:1295-305 (2011). PubMed: 21863214
    • Kim S  et al. The novel endosomal membrane protein Ema interacts with the class C Vps-HOPS complex to promote endosomal maturation. J Cell Biol 188:717-34 (2010). Drosophila melanogaster . PubMed: 20194640
    • Norum M  et al. Trafficking through COPII stabilises cell polarity and drives secretion during Drosophila epidermal differentiation. PLoS One 5:e10802 (2010). IHC-FoFr ; Drosophila melanogaster . PubMed: 20520821

    Customer reviews and Q&As

    Show All Reviews Q&A
    Submit a review Submit a question

    1-4 of 4 Abreviews or Q&A

    Western blot abreview for Anti-KDEL Receptor antibody [KR-10]

    Average
    Abreviews
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Mouse Cell lysate - other (MEF)
    Loading amount
    30 µg
    Specification
    MEF
    Gel Running Conditions
    Reduced Denaturing
    Blocking step
    Milk as blocking agent for 3 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 23°C
    Read More

    Abcam user community

    Verified customer

    Submitted Nov 15 2010

    Western blot abreview for Anti-KDEL Receptor antibody [KR-10]

    Average
    Abreviews
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Human Cell lysate - other (HELA)
    Loading amount
    30 µg
    Specification
    HELA
    Gel Running Conditions
    Reduced Denaturing
    Blocking step
    Milk as blocking agent for 3 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 24°C
    Read More

    Abcam user community

    Verified customer

    Submitted Nov 15 2010

    Immunocytochemistry abreview for Anti-KDEL Receptor antibody [KR-10]

    Below Average
    Abreviews
    Abreviews
    abreview image
    Application
    Immunocytochemistry
    Sample
    Mouse Cultured Cells (Mouse Embryonic Fibroblasts)
    Specification
    Mouse Embryonic Fibroblasts
    Fixative
    Paraformaldehyde
    Permeabilization
    Yes - 0.5% Triton-X
    Blocking step
    BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 24°C
    Read More

    Abcam user community

    Verified customer

    Submitted Oct 15 2010

    Immunocytochemistry abreview for Anti-KDEL Receptor antibody [KR-10]

    Average
    Abreviews
    Abreviews
    abreview image
    Application
    Immunocytochemistry
    Sample
    Human Cultured Cells (HELA)
    Specification
    HELA
    Fixative
    Paraformaldehyde
    Permeabilization
    Yes - 0.5% Triton-X
    Blocking step
    BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 24°C
    Read More

    Abcam user community

    Verified customer

    Submitted Oct 05 2010

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