Product nameAnti-KDEL Receptor antibody [KR-10]
See all KDEL Receptor primary antibodies
DescriptionMouse monoclonal [KR-10] to KDEL Receptor
Tested applicationsSuitable for: ICC/IF, IHC-P, IHC-Fr, IP, IHC-FoFr, WB, Flow Cytmore details
Species reactivityReacts with: Mouse, Rat, Sheep, Rabbit, Chicken, Hamster, Cow, Dog, Human, Pig, Xenopus laevis, Drosophila melanogaster, Monkey
- HeLa cell lysate (heat shocked), 3T3 cell lysate (heat-shocked and unshocked), PC-12 cell lysate (heat shocked and unshocked) and CHO-K1 cell lysate.
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage bufferPreservative: 0.09% Sodium Azide
Constituents: 50% Glycerol, PBS, pH 7.2
Concentration information loading...
PurityProtein G purified
Our Abpromise guarantee covers the use of ab69659 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ICC/IF||Use at an assay dependent concentration.|
|IHC-P||Use at an assay dependent concentration.|
|IHC-Fr||Use at an assay dependent concentration.|
|IP||Use at an assay dependent concentration.|
|IHC-FoFr||Use at an assay dependent concentration. PubMed: 20520821|
|WB||1/1000. Detects a band of approximately 25 kDa (predicted molecular weight: 25 kDa).|
|Flow Cyt||Use 1µg for 106 cells.
ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.
RelevanceRetention of resident soluble proteins in the lumen of the endoplasmic reticulum (ER) is achieved in both yeast and animal cells by their continual retrieval from the cis-Golgi, or a pre-Golgi compartment. Sorting of these proteins is dependent on a C-terminal tetrapeptide signal, usually lys-asp-glu-leu (KDEL) in animal cells, and his-asp-glu-leu (HDEL) in S. cerevisiae. This process is mediated by a receptor that recognizes, and binds the tetrapeptide-containing protein, and returns it to the ER. In yeast, the sorting receptor encoded by a single gene, ERD2, which is a seven-transmembrane protein. Unlike yeast, several human homologs of the ERD2 gene, constituting the KDEL receptor gene family, have been described. The protein encoded by this gene was the first member of the family to be identified, and it encodes a protein structurally and functionally similar to the yeast ERD2 gene product.
Cellular localizationEndoplasmic reticulum
- ER lumen protein retaining receptor 1 antibody
- ERD 2 antibody
- ERD2 antibody
All lanes : Anti-KDEL Receptor antibody [KR-10] (ab69659) at 1/250 dilution
Lane 1 : HeLa cell lysate (heat
Lane 2 : 3T3 cell lysate
Lane 3 : PC-12 cell lysate
Lane 4 : CHO-K1 cell lysate
Lane 5 : 3T3 cell
lysate (heat shocked)
Lane 6 : PC-12 cell lysate
Lysates/proteins at 20 µg per lane.
Predicted band size: 25 kDa
Observed band size: 25 kDa
ab69659 staining KDEL Receptor in Fruitfly epidermal cell tissue by Immunohistochemistry (PFA perfusion fixed frozen sections).
Overlay histogram showing HepG2 cells stained with ab69659 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab69659, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in HepG2 cells fixed with 4% paraformaldehyde (10 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.
This product has been referenced in:
- Bartels AK et al. KDEL Receptor 1 Contributes to Cell Surface Association of Protein Disulfide Isomerases. Cell Physiol Biochem 52:850-868 (2019). Read more (PubMed: 30958660) »
- Lo Piccolo L et al. FUS toxicity is rescued by the modulation of lncRNA hsr? expression in Drosophila melanogaster. Sci Rep 7:15660 (2017). Read more (PubMed: 29142303) »