Anti-KDEL Receptor antibody [KR-10] (ab69659)
- Datasheet
- References (8)
- Protocols
Overview
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Product name
Anti-KDEL Receptor antibody [KR-10]
See all KDEL Receptor primary antibodies -
Description
Mouse monoclonal [KR-10] to KDEL Receptor -
Host species
Mouse -
Tested applications
Suitable for: ICC/IF, IHC-P, IHC-Fr, IP, IHC-FoFr, WB, Flow Cytmore details -
Species reactivity
Reacts with: Mouse, Rat, Sheep, Rabbit, Chicken, Hamster, Cow, Dog, Human, Pig, Xenopus laevis, Drosophila melanogaster, Monkey -
Immunogen
Synthetic peptide corresponding to Cow KDEL Receptor aa 192-212 conjugated to keyhole limpet haemocyanin. 21 residue synthetic peptide.
Sequence:CDFFYLYITKVLKGKKLSLPA
Database link: P33946 -
Positive control
- HeLa cell lysate (heat shocked), 3T3 cell lysate (heat-shocked and unshocked), PC-12 cell lysate (heat shocked and unshocked) and CHO-K1 cell lysate.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
Storage buffer
Preservative: 0.09% Sodium Azide
Constituents: 50% Glycerol, PBS, pH 7.2 -
Concentration information loading... -
Purity
Protein G purified -
Clonality
Monoclonal -
Clone number
KR-10 -
Isotype
IgG1 -
Research areas
Associated products
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Alternative Versions
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Compatible Secondaries
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Conjugation kits
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Corresponding Antibody
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Isotype control
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Recombinant Protein
Applications
Our Abpromise guarantee covers the use of ab69659 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| ICC/IF | Use at an assay dependent concentration. | |
| IHC-P | Use at an assay dependent concentration. | |
| IHC-Fr | Use at an assay dependent concentration. | |
| IP | Use at an assay dependent concentration. | |
| IHC-FoFr | Use at an assay dependent concentration. PubMed: 20520821 | |
| WB | 1/1000. Detects a band of approximately 25 kDa (predicted molecular weight: 25 kDa). | |
| Flow Cyt | Use 1µg for 106 cells. ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.
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Target
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Relevance
Retention of resident soluble proteins in the lumen of the endoplasmic reticulum (ER) is achieved in both yeast and animal cells by their continual retrieval from the cis-Golgi, or a pre-Golgi compartment. Sorting of these proteins is dependent on a C-terminal tetrapeptide signal, usually lys-asp-glu-leu (KDEL) in animal cells, and his-asp-glu-leu (HDEL) in S. cerevisiae. This process is mediated by a receptor that recognizes, and binds the tetrapeptide-containing protein, and returns it to the ER. In yeast, the sorting receptor encoded by a single gene, ERD2, which is a seven-transmembrane protein. Unlike yeast, several human homologs of the ERD2 gene, constituting the KDEL receptor gene family, have been described. The protein encoded by this gene was the first member of the family to be identified, and it encodes a protein structurally and functionally similar to the yeast ERD2 gene product. -
Cellular localization
Endoplasmic reticulum -
Database links
- Entrez Gene: 618184 Cow
- Entrez Gene: 484406 Dog
- Entrez Gene: 10945 Human
- Entrez Gene: 68137 Mouse
- Entrez Gene: 361577 Rat
- Omim: 131235 Human
- SwissProt: P33946 Cow
- SwissProt: P24390 Human
see all -
Alternative names
- ER lumen protein retaining receptor 1 antibody
- ERD 2 antibody
- ERD2 antibody
see all
Images
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![Western blot - Anti-KDEL Receptor antibody [KR-10] (ab69659)](https://www.abcam.com/ps/products/69/ab69659/Images/ab69659.gif)
Western blot - Anti-KDEL Receptor antibody [KR-10] (ab69659)All lanes : Anti-KDEL Receptor antibody [KR-10] (ab69659) at 1/250 dilution
Lane 1 : HeLa cell lysate (heat
shocked)
Lane 2 : 3T3 cell lysate
Lane 3 : PC-12 cell lysate
Lane 4 : CHO-K1 cell lysate
Lane 5 : 3T3 cell
lysate (heat shocked)
Lane 6 : PC-12 cell lysate
(heat shocked)
Lysates/proteins at 20 µg per lane.
Predicted band size: 25 kDa
Observed band size: 25 kDa -
![Immunohistochemistry (PFA perfusion fixed frozen sections) - Anti-KDEL Receptor antibody [KR-10] (ab69659)](https://www.abcam.com/ps/products/69/ab69659/Images/KDEL-Receptor-Primary-antibodies-ab69659-1.jpg)
Immunohistochemistry (PFA perfusion fixed frozen sections) - Anti-KDEL Receptor antibody [KR-10] (ab69659)Image from Norum M et al, PLoS One. 2010 May 24;5(5):e10802, Fig 8.ab69659 staining KDEL Receptor in Fruitfly epidermal cell tissue by Immunohistochemistry (PFA perfusion fixed frozen sections). -
![Flow Cytometry - Anti-KDEL Receptor antibody [KR-10] (ab69659)](https://www.abcam.com/ps/products/69/ab69659/Images/KDEL-Receptor-Primary-antibodies-ab69659-11.jpg)
Flow Cytometry - Anti-KDEL Receptor antibody [KR-10] (ab69659)Overlay histogram showing HepG2 cells stained with ab69659 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab69659, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in HepG2 cells fixed with 4% paraformaldehyde (10 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.
Protocols
Datasheets and documents
References
This product has been referenced in:
- Bartels AK et al. KDEL Receptor 1 Contributes to Cell Surface Association of Protein Disulfide Isomerases. Cell Physiol Biochem 52:850-868 (2019). Read more (PubMed: 30958660) »
- Lo Piccolo L et al. FUS toxicity is rescued by the modulation of lncRNA hsr? expression in Drosophila melanogaster. Sci Rep 7:15660 (2017). Read more (PubMed: 29142303) »
