Anti-KDM1 / LSD1 antibody [1B2E5 + 1B2F2] (ab31954)
- Datasheet
- Specific References (2)
- Protocols
Overview
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Product nameAnti-KDM1 / LSD1 antibody [1B2E5 + 1B2F2]
See all KDM1 / LSD1 primary antibodies -
DescriptionMouse monoclonal [1B2E5 + 1B2F2] to KDM1 / LSD1
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Host speciesMouse
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Tested applicationsSuitable for: IHC-FoFr, IHC-P, IHC-Fr, ELISA, WBmore details
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Species reactivityReacts with: Human
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Immunogen
Recombinant fragment corresponding to Human KDM1/ LSD1.
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Positive control
- HeLa and Jurkat cell lysate for Western blot. Human kidnay and lung carcinoma tissue for Immunohistochemistry.
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General notes
Please note that this antibody is an oligoclonal antibody. It is a cocktail of monoclonal antibodies that have been carefully selected. Oligoclonal antibodies have not only the specificity and batch-to-batch consistency of a monoclonal antibody, but also have the advantage of the sensitivity of a polyclonal antibody due to their ability to recognize multiple epitopes on an antigen.
Properties
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FormLiquid
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Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
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Storage bufferConstituents: Ascites, PBS
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Concentration information loading... -
PurityProtein G purified
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Purification notesPurified from tissue culture supernatant.
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ClonalityMonoclonal
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Clone number1B2E5 + 1B2F2
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IsotypeIgG1
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Research areas
Associated products
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Compatible Secondaries
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Positive Controls
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Related Products
Applications
Our Abpromise guarantee covers the use of ab31954 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| IHC-FoFr | Use at an assay dependent concentration. PubMed: 19817750 | |
| IHC-P | 1/500 - 1/2000. | |
| IHC-Fr | 1/500 - 1/2000. | |
| ELISA | 1/10000. | |
| WB | 1/500 - 1/2000. Predicted molecular weight: 93 kDa. |
Target
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FunctionHistone demethylase that demethylates both 'Lys-4' (H3K4me) and 'Lys-9' (H3K9me) of histone H3, thereby acting as a coactivator or a corepressor, depending on the context. Acts by oxidizing the substrate by FAD to generate the corresponding imine that is subsequently hydrolyzed. Acts as a corepressor by mediating demethylation of H3K4me, a specific tag for epigenetic transcriptional activation. Demethylates both mono- (H3K4me1) and di-methylated (H3K4me2) H3K4me. May play a role in the repression of neuronal genes. Alone, it is unable to demethylate H3K4me on nucleosomes and requires the presence of RCOR1/CoREST to achieve such activity. Also acts as a coactivator of androgen receptor (ANDR)-dependent transcription, by being recruited to ANDR target genes and mediating demethylation of H3K9me, a specific tag for epigenetic transcriptional repression. The presence of PRKCB in ANDR-containing complexes, which mediates phosphorylation of 'Thr-6' of histone H3 (H3T6ph), a specific tag that prevents demethylation H3K4me, prevents H3K4me demethylase activity of KDM1A. Demethylates di-methylated 'Lys-370' of p53/TP53 which prevents interaction of p53/TP53 with TP53BP1 and represses p53/TP53-mediated transcriptional activation. Demethylates and stabilizes the DNA methylase DNMT1. Required for gastrulation during embryogenesis.
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Tissue specificityUbiquitously expressed.
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Sequence similaritiesBelongs to the flavin monoamine oxidase family.
Contains 1 SWIRM domain. -
DomainThe SWIRM domain may act as an anchor site for a histone tail.
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Cellular localizationNucleus.
- Information by UniProt
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Database links
- Entrez Gene: 23028 Human
- Omim: 609132 Human
- SwissProt: O60341 Human
- Unigene: 591518 Human
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Alternative names
- Amine oxidase (flavin containing) domain 2 antibody
- Amine oxidase, flavin containing, 2 antibody
- AOF2 antibody
see all
Images
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![Western blot - Anti-KDM1 / LSD1 antibody [1B2E5 + 1B2F2] (ab31954)](https://www.abcam.com/ps/products/31/ab31954/Images/ab31954-266785-ab31954KOWB.jpg)
Western blot - Anti-KDM1 / LSD1 antibody [1B2E5 + 1B2F2] (ab31954)Lane 1: Wild-type HAP1 cell lysate (20 µg)
Lane 2: KDM1/LSD1 knockout HAP1 cell lysate (20 µg)
Lane 3: HeLa cell lysate (20 µg)
Lane 4: Jurkat cell lysate (20 µg)
Lanes 1 - 4: Merged signal (red and green). Green - ab31954 observed at 110 kDa. Red - loading control, ab181602, observed at 37 kDa.
ab31954 was shown to specifically recognize KDM1/LSD1 in wild-type HAP1 cells along with additional cross reactive bands. No bands were observed when KDM1/LSD1 knockout samples were used. Wild-type and KDM1/LSD1 knockout samples were subjected to SDS-PAGE. ab31954 and ab181602 (loading control to GAPDH) were diluted 1/250 and 1/10,000 respectively and incubated overnight at 4°C. Blots were developed with Goat anti-Mouse IgG H&L (IRDye® 800CW) preadsorbed (ab216772) and Goat Anti-Rabbit IgG H&L (IRDye® 680RD) preadsorbed (ab216777) secondary antibodies at 1/10,000 dilution for 1hr at room temperature before imaging. -
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-KDM1 / LSD1 antibody [1B2E5 + 1B2F2] (ab31954)](https://www.abcam.com/ps/products/31/ab31954/Images/ab31954_IHC_1.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-KDM1 / LSD1 antibody [1B2E5 + 1B2F2] (ab31954)Immunohistochemical analysis of paraffin-embedded human lung carcinoma tissue, showing nuclear localisation using LSD1 antibody (ab31954) dilution 1:500-1:2000 and an HRP-conjugated secondary antibody with DAB staining. -
![Western blot - Anti-KDM1 / LSD1 antibody [1B2E5 + 1B2F2] (ab31954)](https://www.abcam.com/ps/products/31/ab31954/Images/ab31954_WB.jpg)
Western blot - Anti-KDM1 / LSD1 antibody [1B2E5 + 1B2F2] (ab31954)All lanes : Anti-KDM1 / LSD1 antibody [1B2E5 + 1B2F2] (ab31954)
Lane 1 : Molecular weight marker
Lane 2 : LSD1 recombinant protein (NB: truncated form)
Lane 3 : HeLa cell lysate
Predicted band size: 93 kDa
Primary antibody dilution range 1:500-1:2000. -
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-KDM1 / LSD1 antibody [1B2E5 + 1B2F2] (ab31954)](https://www.abcam.com/ps/products/31/ab31954/Images/ab31954_IHC_2.gif)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-KDM1 / LSD1 antibody [1B2E5 + 1B2F2] (ab31954)Immunohistochemical analysis of paraffin-embedded human kidney carcinoma tissue, showing nuclear localisation using LSD1 antibody (ab31954) dilution 1:500-1:2000 and an HRP-conjugated secondary antibody with DAB staining.
References
This product has been referenced in:
- Yokoyama A et al. Identification of Myelin Transcription Factor 1 (MyT1) as a Subunit of the Neural Cell Type-specific Lysine-specific Demethylase 1 (LSD1) Complex. J Biol Chem 289:18152-18162 (2014). Read more (PubMed: 24828497) »
- Hirono S et al. Molecular markers associated with lymph node metastasis in pancreatic ductal adenocarcinoma by genome-wide expression profiling. Cancer Sci : (2009). IHC-FoFr ; Human . Read more (PubMed: 19817750) »
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