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I am working for Dana Farber Cancer Institute, Boston, MA.Our labis looking for a ELISA kit which can measure the activity of LSD1.For this, we are trying to understand the difference between all the 4 ELISA kits for LSD1(named below) ABcam is providing. 1. EpiSeeker Histone Demethylase KDM1/LSD1 Activity Quantification Assay Kit (ab113457) 2. EpiSeeker KDM1/LSD1 Activity Quantification Kit (Colorimetric) ab113459 3. EpiSeeker Histone Demethylase KDM1/LSD1 Inhibitor Assay Kit (ab113456) 4. EpiSeeker KDM1/LSD1 Activity Quantification Assay Kit (Fluorometric) (ab113460) Pleaselet us know the differentpurposes of each kit andhow to choose the correct kit for our experiments. Thanks in advace
Asked on Jan 09 2012
Thank you for contacting Abcam. For ab113459 and ab113460, both kits directly detects KDM1/LSD1-converted demethylated products, rather than by-products, in a 96 stripwell microplate format. The main difference between the 2 are that ab113459, has a colorimetric detection systems, whereas, ab113460 has a fluorescent detections method. Depending on the equipment available in your lab, would determine which one of these you would choose. The ab113457 measures histone demethylase KDM1/LSD1 activity/inhibition from a broad range of species including mammalian cells/tissues, plants, and bacteria and this is an indirect detection method, compared to the above kits. For ab113456, this kit is ready-to-use and provides all the essential components needed for screening KDM1/LSD1 inhibitors, which are directly interacting with the enzyme or blocking the binding to its substrate. You can find more information about these products and how to choose the correct kit for your needs, by clicking on the following link: https://www.abcam.com/index.htmlpageconfig=resource&rid=14469&source=pagetrap&viapagetrap=episeeker#3 If there is anything else I can help you with, please let me know.
Answered on Jan 09 2012