Key features and details
- Mouse Monoclonal [1B2E5 + 1B2F2] to KDM1/LSD1
- Suitable for: IHC-P, WB
- Knockout validated
- Reacts with: Human
- Isotype: IgG1
Product nameAnti-KDM1/LSD1 antibody [1B2E5 + 1B2F2]
See all KDM1/LSD1 primary antibodies
DescriptionMouse Monoclonal [1B2E5 + 1B2F2] to KDM1/LSD1
Tested applicationsSuitable for: IHC-P, WBmore details
Species reactivityReacts with: Human
Recombinant fragment corresponding to Human KDM1/ LSD1.
- HeLa and Jurkat cell lysate for Western blot. Human kidnay and lung carcinoma tissue for Immunohistochemistry.
Please note that this antibody is an oligoclonal antibody. It is a cocktail of monoclonal antibodies that have been carefully selected. Oligoclonal antibodies have not only the specificity and batch-to-batch consistency of a monoclonal antibody, but also have the advantage of the sensitivity of a polyclonal antibody due to their ability to recognize multiple epitopes on an antigen.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage bufferPreservative: 0.05% Sodium azide
Concentration information loading...
PurityProtein G purified
Purification notesPurified from tissue culture supernatant.
Clone number1B2E5 + 1B2F2
Our Abpromise guarantee covers the use of ab31954 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||1/500 - 1/2000.|
|WB||1/500 - 1/2000. Predicted molecular weight: 93 kDa.|
FunctionHistone demethylase that demethylates both 'Lys-4' (H3K4me) and 'Lys-9' (H3K9me) of histone H3, thereby acting as a coactivator or a corepressor, depending on the context. Acts by oxidizing the substrate by FAD to generate the corresponding imine that is subsequently hydrolyzed. Acts as a corepressor by mediating demethylation of H3K4me, a specific tag for epigenetic transcriptional activation. Demethylates both mono- (H3K4me1) and di-methylated (H3K4me2) H3K4me. May play a role in the repression of neuronal genes. Alone, it is unable to demethylate H3K4me on nucleosomes and requires the presence of RCOR1/CoREST to achieve such activity. Also acts as a coactivator of androgen receptor (ANDR)-dependent transcription, by being recruited to ANDR target genes and mediating demethylation of H3K9me, a specific tag for epigenetic transcriptional repression. The presence of PRKCB in ANDR-containing complexes, which mediates phosphorylation of 'Thr-6' of histone H3 (H3T6ph), a specific tag that prevents demethylation H3K4me, prevents H3K4me demethylase activity of KDM1A. Demethylates di-methylated 'Lys-370' of p53/TP53 which prevents interaction of p53/TP53 with TP53BP1 and represses p53/TP53-mediated transcriptional activation. Demethylates and stabilizes the DNA methylase DNMT1. Required for gastrulation during embryogenesis.
Tissue specificityUbiquitously expressed.
Sequence similaritiesBelongs to the flavin monoamine oxidase family.
Contains 1 SWIRM domain.
DomainThe SWIRM domain may act as an anchor site for a histone tail.
- Information by UniProt
- Amine oxidase (flavin containing) domain 2 antibody
- Amine oxidase, flavin containing, 2 antibody
- AOF2 antibody
Lane 1: Wild-type HAP1 cell lysate (20 µg)
Lane 2: KDM1/LSD1 knockout HAP1 cell lysate (20 µg)
Lane 3: HeLa cell lysate (20 µg)
Lane 4: Jurkat cell lysate (20 µg)
Lanes 1 - 4: Merged signal (red and green). Green - ab31954 observed at 110 kDa. Red - loading control, ab181602, observed at 37 kDa.
ab31954 was shown to specifically recognize KDM1/LSD1 in wild-type HAP1 cells along with additional cross reactive bands. No bands were observed when KDM1/LSD1 knockout samples were used. Wild-type and KDM1/LSD1 knockout samples were subjected to SDS-PAGE. ab31954 and ab181602 (loading control to GAPDH) were diluted 1/250 and 1/10,000 respectively and incubated overnight at 4°C. Blots were developed with Goat anti-Mouse IgG H&L (IRDye® 800CW) preadsorbed (ab216772) and Goat Anti-Rabbit IgG H&L (IRDye® 680RD) preadsorbed (ab216777) secondary antibodies at 1/10,000 dilution for 1hr at room temperature before imaging.
Immunohistochemical analysis of paraffin-embedded human lung carcinoma tissue, showing nuclear localisation using LSD1 antibody (ab31954) dilution 1:500-1:2000 and an HRP-conjugated secondary antibody with DAB staining.
All lanes : Anti-KDM1/LSD1 antibody [1B2E5 + 1B2F2] (ab31954)
Lane 1 : Molecular weight marker
Lane 2 : LSD1 recombinant protein (NB: truncated form)
Lane 3 : HeLa cell lysate
Predicted band size: 93 kDa
Primary antibody dilution range 1:500-1:2000.
Immunohistochemical analysis of paraffin-embedded human kidney carcinoma tissue, showing nuclear localisation using LSD1 antibody (ab31954) dilution 1:500-1:2000 and an HRP-conjugated secondary antibody with DAB staining.
ab31954 has been referenced in 2 publications.
- Yokoyama A et al. Identification of Myelin Transcription Factor 1 (MyT1) as a Subunit of the Neural Cell Type-specific Lysine-specific Demethylase 1 (LSD1) Complex. J Biol Chem 289:18152-18162 (2014). PubMed: 24828497
- Hirono S et al. Molecular markers associated with lymph node metastasis in pancreatic ductal adenocarcinoma by genome-wide expression profiling. Cancer Sci : (2009). IHC-FoFr ; Human . PubMed: 19817750