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  1. Link

    kdm1lsd1-antibody-ab62582.pdf

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Epigenetics and Nuclear Signaling Chromatin Modifying Enzymes Methylation Lysine demethylation
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Anti-KDM1/LSD1 antibody (ab62582)

  • Datasheet
Reviews (1)Q&A (2)References (4)

Product price, shipping and contact information

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Western blot - Anti-KDM1/LSD1 antibody (ab62582)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-KDM1/LSD1 antibody (ab62582)
  • Immunocytochemistry/ Immunofluorescence - Anti-KDM1/LSD1 antibody (ab62582)
  • Western blot - Anti-KDM1 / LSD1 antibody (ab62582)

Key features and details

  • Rabbit polyclonal to KDM1/LSD1
  • Suitable for: ICC/IF, IHC-P, WB
  • Reacts with: Mouse, Human
  • Isotype: IgG

You may also be interested in

Secondary
Product image
Goat Anti-Rabbit IgG H&L (HRP) (ab205718)

View more associated products

Overview

  • Product name

    Anti-KDM1/LSD1 antibody
    See all KDM1/LSD1 primary antibodies
  • Description

    Rabbit polyclonal to KDM1/LSD1
  • Host species

    Rabbit
  • Tested Applications & Species

    Application Species
    ICC/IF
    Human
    IHC-P
    Human
    WB
    Mouse
    Human
    See all applications and species data
  • Immunogen

    Synthetic 16 amino acid peptide from near the centre of human KDM1/ LSD1.

  • Positive control

    • WB: MCF-7, Jurkat, and P815 cell lysates. IHC-P: Human small intestine tissue.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.2
    Preservative: 0.02% Sodium azide
    Constituent: PBS
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

    • Epigenetics and Nuclear Signaling
    • Chromatin Modifying Enzymes
    • Methylation
    • Lysine demethylation

Associated products

  • Compatible Secondaries

    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
    • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
  • Isotype control

    • Rabbit IgG, polyclonal - Isotype Control (ChIP Grade) (ab171870)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab62582 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Guaranteed

Tested applications are guaranteed to work and covered by our Abpromise guarantee.

Predicted

Predicted to work for this combination of applications and species but not guaranteed.

Incompatible

Does not work for this combination of applications and species.

Application Species
ICC/IF
Human
IHC-P
Human
WB
Mouse
Human
Application Abreviews Notes
ICC/IF
Use a concentration of 20 µg/ml.
IHC-P
Use a concentration of 2 µg/ml.
WB (1)
Use a concentration of 0.2 - 2 µg/ml. Detects a band of approximately 105 kDa (predicted molecular weight: 93 kDa).
Notes
ICC/IF
Use a concentration of 20 µg/ml.
IHC-P
Use a concentration of 2 µg/ml.
WB
Use a concentration of 0.2 - 2 µg/ml. Detects a band of approximately 105 kDa (predicted molecular weight: 93 kDa).

Target

  • Function

    Histone demethylase that demethylates both 'Lys-4' (H3K4me) and 'Lys-9' (H3K9me) of histone H3, thereby acting as a coactivator or a corepressor, depending on the context. Acts by oxidizing the substrate by FAD to generate the corresponding imine that is subsequently hydrolyzed. Acts as a corepressor by mediating demethylation of H3K4me, a specific tag for epigenetic transcriptional activation. Demethylates both mono- (H3K4me1) and di-methylated (H3K4me2) H3K4me. May play a role in the repression of neuronal genes. Alone, it is unable to demethylate H3K4me on nucleosomes and requires the presence of RCOR1/CoREST to achieve such activity. Also acts as a coactivator of androgen receptor (ANDR)-dependent transcription, by being recruited to ANDR target genes and mediating demethylation of H3K9me, a specific tag for epigenetic transcriptional repression. The presence of PRKCB in ANDR-containing complexes, which mediates phosphorylation of 'Thr-6' of histone H3 (H3T6ph), a specific tag that prevents demethylation H3K4me, prevents H3K4me demethylase activity of KDM1A. Demethylates di-methylated 'Lys-370' of p53/TP53 which prevents interaction of p53/TP53 with TP53BP1 and represses p53/TP53-mediated transcriptional activation. Demethylates and stabilizes the DNA methylase DNMT1. Required for gastrulation during embryogenesis.
  • Tissue specificity

    Ubiquitously expressed.
  • Sequence similarities

    Belongs to the flavin monoamine oxidase family.
    Contains 1 SWIRM domain.
  • Domain

    The SWIRM domain may act as an anchor site for a histone tail.
  • Cellular localization

    Nucleus.
  • Target information above from: UniProt accession O60341 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 23028 Human
    • Entrez Gene: 99982 Mouse
    • Omim: 609132 Human
    • SwissProt: O60341 Human
    • SwissProt: Q6ZQ88 Mouse
    • Unigene: 591518 Human
    • Unigene: 28540 Mouse
    • Alternative names

      • Amine oxidase (flavin containing) domain 2 antibody
      • Amine oxidase, flavin containing, 2 antibody
      • AOF2 antibody
      • BHC110 antibody
      • BRAF35 HDAC complex protein BHC110 antibody
      • BRAF35-HDAC complex protein BHC110 antibody
      • BRAF35/HDAC complex, 110-kD subunit antibody
      • CPRF antibody
      • EC1 antibody
      • FAD binding protein BRAF35 HDAC complex, 110 kDa subunit antibody
      • Flavin-containing amine oxidase domain-containing protein 2 antibody
      • KDM 1 antibody
      • KDM1 antibody
      • Kdm1a antibody
      • KDM1A_HUMAN antibody
      • KIAA0601 antibody
      • LSD 1 antibody
      • LSD1 antibody
      • Lysine (K) specific demethylase 1 antibody
      • Lysine (K) specific demethylase 1A antibody
      • Lysine demethylase 1A antibody
      • Lysine specific histone demethylase 1 antibody
      • Lysine specific histone demethylase 1A antibody
      • Lysine-specific demethylase 1 antibody
      • Lysine-specific demethylase 1A antibody
      • Lysine-specific histone demethylase 1A antibody
      see all

    Images

    • Western blot - Anti-KDM1/LSD1 antibody (ab62582)
      Western blot - Anti-KDM1/LSD1 antibody (ab62582)
      All lanes : Anti-KDM1/LSD1 antibody (ab62582) at 1 µg/ml

      Lane 1 : MCF7 (human breast adenocarcinoma cell line) cell lysate
      Lane 2 : Jurkat (human T cell leukemia cell line from peripheral blood) cell lysate

      Lysates/proteins at 15 µg per lane.

      Secondary
      All lanes : Goat anti-rabbit IgG (HRP) at 1/10000 dilution

      Predicted band size: 93 kDa
      Observed band size: 110 kDa
      why is the actual band size different from the predicted?



      Diluting buffer and concentration: 5% NFDM/TBST.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-KDM1/LSD1 antibody (ab62582)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-KDM1/LSD1 antibody (ab62582)

      Immunohistochemical analysis of paraffin-embedded human small intestine tissue using ab62582 at 2 μg/ml. Tissue was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4°C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.

    • Immunocytochemistry/ Immunofluorescence - Anti-KDM1/LSD1 antibody (ab62582)
      Immunocytochemistry/ Immunofluorescence - Anti-KDM1/LSD1 antibody (ab62582)
      Immunofluorescence of LSD1 in Human Small Intestine cells using ab62582 at 20 ug/ml.

    • Western blot - Anti-KDM1 / LSD1 antibody (ab62582)
      Western blot - Anti-KDM1 / LSD1 antibody (ab62582)
      Lane 1 : Anti-KDM1/LSD1 antibody (ab62582) at 1 µg/ml
      Lane 2 : Anti-KDM1/LSD1 antibody (ab62582) at 2 µg/ml

      All lanes : P815 cell lysate

      Lysates/proteins at 15 µg per lane.

      Predicted band size: 93 kDa
      Observed band size: 105 kDa why is the actual band size different from the predicted?
      Additional bands at: 60 kDa. We are unsure as to the identity of these extra bands.

    Protocols

    • Western blot protocols
    • Immunohistochemistry protocols
    • Immunocytochemistry & immunofluorescence protocols

    Click here to view the general protocols

    Datasheets and documents

    • Datasheet
  • References (4)

    Publishing research using ab62582? Please let us know so that we can cite the reference in this datasheet.

    ab62582 has been referenced in 4 publications.

    • Chen Z  et al. Two-layer regulation of TRAF6 mediated by both TLR4/NF-kB signaling and miR-589-5p increases proinflammatory cytokines in the pathology of severe acute pancreatitis. Am J Transl Res 12:2379-2395 (2020). PubMed: 32655778
    • Desai M  et al. Programmed hyperphagia in offspring of obese dams: Altered expression of hypothalamic nutrient sensors, neurogenic factors and epigenetic modulators. Appetite 99:193-199 (2016). PubMed: 26785315
    • Gao YQ  et al. Regulation of DLK1 by the maternally expressed miR-379/miR-544 cluster may underlie callipyge polar overdominance inheritance. Proc Natl Acad Sci U S A 112:13627-32 (2015). PubMed: 26487685
    • Krolewski RC  et al. Global expression profiling of globose basal cells and neurogenic progression within the olfactory epithelium. J Comp Neurol : (2012). WB ; Mouse . PubMed: 22847514

    Customer reviews and Q&As

    Show All Reviews Q&A
    Submit a review Submit a question

    1-3 of 3 Abreviews or Q&A

    Question

    IHC-Fr with rat brain - images for glial cells?
    we do not have images for glial cell staining

    ab129195: blocking peptide available?
    ab62582: blocking peptide available?

    Read More

    Abcam community

    Verified customer

    Asked on Feb 27 2013

    Answer


    The blocking peptide for ab62582 has been added as KDM1 / LSD1 peptide (ab166968).

    https://www.abcam.com/KDM1--LSD1-peptide-ab166968.html

    Read More

    Abcam Scientific Support

    Answered on Feb 27 2013

    Question

    IHC-Fr with rat brain - images for glial cells?
    we do not have images for glial cell staining

    ab129195: blocking peptide available?
    ab62582: blocking peptide available?

    Read More

    Abcam community

    Verified customer

    Asked on Feb 26 2013

    Answer


    As for the blocking peptides:

    For ab129195: The blocking peptide is now available as ab166919.
    https://www.abcam.com/KDM1-LSD1-peptide-ab166919.html

    For ab62582: This will be available soon and I will let you know when I have a catalog number and price.

    Read More

    Abcam Scientific Support

    Answered on Feb 26 2013

    Western blot abreview for Anti-KDM1 / LSD1 antibody

    Average
    Abreviews
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Xenopus laevis Cell lysate - whole cell (egg extracts)
    Loading amount
    20 µg
    Specification
    egg extracts
    Gel Running Conditions
    Reduced Denaturing
    Blocking step
    Milk as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: RT°C
    Read More

    Abcam user community

    Verified customer

    Submitted Dec 21 2012

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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