For the best experience on the Abcam website please upgrade to a modern browser such as Google Chrome

We use cookies to make our site as useful as possible.

Our Cookie Policy explains how you can opt-out of the cookies we use.

If you continue without changing your cookie settings, we'll assume you’re happy with this.

Continue Continue

United States
Your country/region is currently set to:

If incorrect, please enter your country/region into the box below, to view site information related to your country/region.

Call (888) 77-ABCAM (22226) or contact us
Need help? Contact us

  • My account
  • Sign out
Sign in or Register with us

Welcome

Sign in or

Don't have an account?

Register with us
My basket
Quick order
Abcam homepage

  • Research Products
    By product type
    Primary antibodies
    Secondary antibodies
    ELISA and Matched Antibody Pair Kits
    Cell and tissue imaging tools
    Cellular and biochemical assays
    Proteins and Peptides
    By product type
    Proteomics tools
    Agonists, activators, antagonists and inhibitors
    Cell lines and Lysates
    Multiplex miRNA assays
    Multiplex Assays
    By research area
    Cancer
    Cardiovascular
    Cell Biology
    Epigenetics
    Metabolism
    Developmental Biology
    By research area
    Immunology
    Microbiology
    Neuroscience
    Signal Transduction
    Stem Cells
  • Diagnostic & Therapeutic Solutions
    Custom solutions & partnerships

    Custom antibody development and commercial partnerships to advance your diagnostic and therapeutic discovery.

    Create custom solutions with us

    Partner with us

  • Support
    Support hub

    Access advice and support for any research roadblock

    View support hub

    Protocols

    Your experiments laid out step by step

    View protocols

  • Events
    • Conference calendar
    • Cancer
    • Cardiovascular
    • Epigenetics & Nuclear signaling
    • Immunology
    • Neuroscience
    • Stem cells
    • Tradeshows
    • Scientific webinars
    Keep up to date with the latest events

    Full event breakdown with abstracts, speakers, registration and more

    View global event calendar

  • Pathways
    Cell signalling pathways

    View all pathways

    View all interactive pathways

Supporting our customers and employees during the COVID-19 pandemic. Read more

  1. Link

    kdm1lsd1-antibody-epr6825-bsa-and-azide-free-ab224270.pdf

  1. Send me a copy of this email
    I agree to the terms and conditions.
Epigenetics and Nuclear Signaling Chromatin Modifying Enzymes Methylation Lysine demethylation
Share by email
Validated using a knockout cell lineRecombinantRabMAb

Recombinant Anti-KDM1/LSD1 antibody [EPR6825] - BSA and Azide free (ab224270)

  • Datasheet
  • Certificate of Compliance
Submit a review Submit a question References (3)

Product price, shipping and contact information

Currently unavailable

Sorry, we can't display this right now.

Please contact us to place your order, or try again later.

 

Loading size & price…

 

Shipping and order information

Abpromise

Guaranteed product quality, expert customer support.

Find out more.

Western blot - Anti-KDM1/LSD1 antibody [EPR6825] - BSA and Azide free (ab224270)
  • ChIP - Anti-KDM1/LSD1 antibody [EPR6825] - BSA and Azide free (ab224270)
  • Immunocytochemistry/ Immunofluorescence - Anti-KDM1 / LSD1 antibody [EPR6825] - BSA and Azide free (ab224270)
  • Western blot - Anti-KDM1/LSD1 antibody [EPR6825] - BSA and Azide free (ab224270)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-KDM1 / LSD1 antibody [EPR6825] - BSA and Azide free (ab224270)
  • Immunocytochemistry/ Immunofluorescence - Anti-KDM1 / LSD1 antibody [EPR6825] - BSA and Azide free (ab224270)
  • Flow Cytometry - Anti-KDM1 / LSD1 antibody [EPR6825] - BSA and Azide free (ab224270)
  • Immunoprecipitation - Anti-KDM1 / LSD1 antibody [EPR6825] - BSA and Azide free (ab224270)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-KDM1 / LSD1 antibody [EPR6825] - BSA and Azide free (ab224270)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-KDM1 / LSD1 antibody [EPR6825] - BSA and Azide free (ab224270)
  • Immunocytochemistry/ Immunofluorescence - Anti-KDM1/LSD1 antibody [EPR6825] - BSA and Azide free (ab224270)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-KDM1 / LSD1 antibody [EPR6825] - BSA and Azide free (ab224270)
  • Anti-KDM1/LSD1 antibody [EPR6825] - BSA and Azide free (ab224270)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR6825] to KDM1/LSD1 - BSA and Azide free
  • Suitable for: ChIP, ICC/IF, WB, IHC-P, IP, Flow Cyt
  • Knockout validated
  • Reacts with: Mouse, Rat, Human

You may also be interested in

Conjugation
Product image
PE / R-Phycoerythrin Conjugation Kit - Lightning-Link® (ab102918)
Primary
Product image
Anti-Histone H3 (acetyl K9) antibody [EPR16988] - ChIP Grade (ab177177)
Knockout
Product image
Human KDM1A (LSD1) knockout HeLa cell line (ab265790)

View more associated products

Overview

  • Product name

    Anti-KDM1/LSD1 antibody [EPR6825] - BSA and Azide free
    See all KDM1/LSD1 primary antibodies
  • Description

    Rabbit monoclonal [EPR6825] to KDM1/LSD1 - BSA and Azide free
  • Host species

    Rabbit
  • Tested applications

    Suitable for: ChIP, ICC/IF, WB, IHC-P, IP, Flow Cytmore details
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) corresponding to Human KDM1/ LSD1 aa 50-150.
    (Peptide available as ab166919)

  • Positive control

    • WB: HAP1, 293T, HEK293, HeLa, Jurkat, PC3, C6, Raw 264.7, PC-12, and NIH 3T3 cell lysates. IHC-P: Human testis, rat kidney, and mouse colon tissues. ICC/IF: HAP1 and HeLa cells. Flow Cyt: HeLa cells. IP: Jurkat cell lysate. ChIP: HCT 116 (Human colorectal carcinoma epithelial cell).
  • General notes

    ab224270 is the carrier-free version of ab129195. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

    ab224270 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C. Do Not Freeze.
  • Storage buffer

    pH: 7.2
    Constituent: PBS
  • Carrier free

    Yes
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Monoclonal
  • Clone number

    EPR6825
  • Isotype

    IgG
  • Research areas

    • Epigenetics and Nuclear Signaling
    • Chromatin Modifying Enzymes
    • Methylation
    • Lysine demethylation

Associated products

  • Alternative Versions

    • Anti-KDM1/LSD1 antibody [EPR6825] - Nuclear Marker and ChIP Grade (ab129195)
    • Alexa Fluor® 488 Anti-KDM1/LSD1 antibody [EPR6825] - Nuclear Marker (ab184811)
    • Alexa Fluor® 647 Anti-KDM1/LSD1 antibody [EPR6825] - Nuclear Marker (ab195305)
    • HRP Anti-KDM1/LSD1 antibody [EPR6825] - Nuclear Marker (ab195897)
  • Compatible Secondaries

    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
    • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
    • Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773)
  • Conjugation kits

    • PE / R-Phycoerythrin Conjugation Kit - Lightning-Link® (ab102918)
    • APC Conjugation Kit - Lightning-Link® (ab201807)
  • Immunizing Peptide (Blocking)

    • KDM1 / LSD1 peptide (ab166919)
  • Isotype control

    • Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730)
  • KO cell lines

    • Human KDM1A (LSD1) knockout HeLa cell line (ab265790)
  • KO cell lysates

    • Human KDM1A (LSD1) knockout HeLa cell lysate (ab256965)
  • Positive Controls

    • HeLa nuclear extract lysate (ab14655)
    • NIH 3T3 nuclear extract lysate (ab14874)
    • PC12 nuclear extract lysate (ab14884)

Applications

Our Abpromise guarantee covers the use of ab224270 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ChIP Use at an assay dependent concentration.
ICC/IF Use at an assay dependent concentration.
WB Use at an assay dependent concentration. Detects a band of approximately 110 kDa (predicted molecular weight: 92 kDa).Can be blocked with KDM1 / LSD1 peptide (ab166919).
IHC-P Use at an assay dependent concentration. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
IP Use at an assay dependent concentration.
Flow Cyt Use at an assay dependent concentration.

ab199376 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

Target

  • Function

    Histone demethylase that demethylates both 'Lys-4' (H3K4me) and 'Lys-9' (H3K9me) of histone H3, thereby acting as a coactivator or a corepressor, depending on the context. Acts by oxidizing the substrate by FAD to generate the corresponding imine that is subsequently hydrolyzed. Acts as a corepressor by mediating demethylation of H3K4me, a specific tag for epigenetic transcriptional activation. Demethylates both mono- (H3K4me1) and di-methylated (H3K4me2) H3K4me. May play a role in the repression of neuronal genes. Alone, it is unable to demethylate H3K4me on nucleosomes and requires the presence of RCOR1/CoREST to achieve such activity. Also acts as a coactivator of androgen receptor (ANDR)-dependent transcription, by being recruited to ANDR target genes and mediating demethylation of H3K9me, a specific tag for epigenetic transcriptional repression. The presence of PRKCB in ANDR-containing complexes, which mediates phosphorylation of 'Thr-6' of histone H3 (H3T6ph), a specific tag that prevents demethylation H3K4me, prevents H3K4me demethylase activity of KDM1A. Demethylates di-methylated 'Lys-370' of p53/TP53 which prevents interaction of p53/TP53 with TP53BP1 and represses p53/TP53-mediated transcriptional activation. Demethylates and stabilizes the DNA methylase DNMT1. Required for gastrulation during embryogenesis.
  • Tissue specificity

    Ubiquitously expressed.
  • Sequence similarities

    Belongs to the flavin monoamine oxidase family.
    Contains 1 SWIRM domain.
  • Domain

    The SWIRM domain may act as an anchor site for a histone tail.
  • Cellular localization

    Nucleus.
  • Target information above from: UniProt accession O60341 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 23028 Human
    • Entrez Gene: 99982 Mouse
    • Entrez Gene: 500569 Rat
    • Omim: 609132 Human
    • SwissProt: O60341 Human
    • SwissProt: Q6ZQ88 Mouse
    • Unigene: 591518 Human
    • Unigene: 28540 Mouse
    • Unigene: 203461 Rat
    see all
  • Alternative names

    • Amine oxidase (flavin containing) domain 2 antibody
    • Amine oxidase, flavin containing, 2 antibody
    • AOF2 antibody
    • BHC110 antibody
    • BRAF35 HDAC complex protein BHC110 antibody
    • BRAF35-HDAC complex protein BHC110 antibody
    • BRAF35/HDAC complex, 110-kD subunit antibody
    • CPRF antibody
    • EC1 antibody
    • FAD binding protein BRAF35 HDAC complex, 110 kDa subunit antibody
    • Flavin-containing amine oxidase domain-containing protein 2 antibody
    • KDM 1 antibody
    • KDM1 antibody
    • Kdm1a antibody
    • KDM1A_HUMAN antibody
    • KIAA0601 antibody
    • LSD 1 antibody
    • LSD1 antibody
    • Lysine (K) specific demethylase 1 antibody
    • Lysine (K) specific demethylase 1A antibody
    • Lysine demethylase 1A antibody
    • Lysine specific histone demethylase 1 antibody
    • Lysine specific histone demethylase 1A antibody
    • Lysine-specific demethylase 1 antibody
    • Lysine-specific demethylase 1A antibody
    • Lysine-specific histone demethylase 1A antibody
    see all

Images

  • Western blot - Anti-KDM1/LSD1 antibody [EPR6825] - BSA and Azide free (ab224270)
    Western blot - Anti-KDM1/LSD1 antibody [EPR6825] - BSA and Azide free (ab224270)
    All lanes : Anti-KDM1/LSD1 antibody [EPR6825] - Nuclear Marker and ChIP Grade (ab129195) at 1/1000 dilution

    Lane 1 : Wild-type HeLa cell lysate
    Lane 2 : KDM1 knockout HeLa cell lysate

    Lysates/proteins at 20 µg per lane.

    Performed under reducing conditions.

    Predicted band size: 92 kDa
    Observed band size: 110 kDa
    why is the actual band size different from the predicted?



    This data was developed using the same antibody clone in a different buffer formulation (ab129195).

      Lanes 1- 2: Merged signal (red and green). Green - ab129195 observed at 110 kDa. Red - Anti-GAPDH antibody [6C5] - Loading Control (ab8245) observed at 37 kDa.

     ab129195 was shown to react with KDM1/LSD1 in wild-type HeLa cells in western blot. Loss of signal was observed when knockout cell line ab265790 (knockout cell lysate ab256965) was used. Wild-type HeLa and KDM1 knockout HeLa cell lysates were subjected to SDS-PAGE. Membrane was blocked for 1 hour at room temperature in 0.1% TBST with 3% non-fat dried milk. ab129195 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) overnight at 4°C at a 1 in 1000 dilution and a 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye®800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye®680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.

  • ChIP - Anti-KDM1/LSD1 antibody [EPR6825] - BSA and Azide free (ab224270)
    ChIP - Anti-KDM1/LSD1 antibody [EPR6825] - BSA and Azide free (ab224270)

    Chromatin was prepared from HCT 116 cells according to the Abcam X-ChIP protocol. Cells were fixed with 1% formaldehyde for 10 minutes. The ChIP was performed with 25µg of chromatin, 5µg of ab129195 (red), and 20µl of protein A/G sepharose beads slurry (10µl of sepharose A beads + 10µl of sepharose G beads). 5μg of rabbit normal IgG was added to the beads control (grey). The immunoprecipitated DNA was quantified by real time PCR (Sybr green approach).

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab129195).

  • Immunocytochemistry/ Immunofluorescence - Anti-KDM1 / LSD1 antibody [EPR6825] - BSA and Azide free (ab224270)
    Immunocytochemistry/ Immunofluorescence - Anti-KDM1 / LSD1 antibody [EPR6825] - BSA and Azide free (ab224270)

    This ICC data was generated using the same anti-KDM1/LSD! antibody clone [EPR6825] in a different buffer formulation (cat# ab129195).

    ab129195 staining KDM1A/LSD1 in wild-type HAP1 cells (top panel) and KDM1A knockout HAP1 cells (bottom panel). The cells were fixed with 4% formaldehyde (10min), permeabilized with 0.1% Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated with ab129195 at 1μg/ml concentration and ab195889 at 1/250 dilution (shown in pseudo colour red) overnight at +4°C, followed by a further incubation at room temperature for 1h with a goat secondary antibody to Rabbit IgG (Alexa Fluor® 488) (ab150081) at 2 μg/ml (shown in green). Nuclear DNA was labelled in blue with DAPI.

  • Western blot - Anti-KDM1/LSD1 antibody [EPR6825] - BSA and Azide free (ab224270)
    Western blot - Anti-KDM1/LSD1 antibody [EPR6825] - BSA and Azide free (ab224270)
    All lanes : Anti-KDM1/LSD1 antibody [EPR6825] - Nuclear Marker and ChIP Grade (ab129195) at 1/10000 dilution

    Lane 1 : Wild-type HAP1 cell lysate
    Lane 2 : KMD1 / LSD1 knockout HAP1 cell lysate
    Lane 3 : HeLa cell lysate
    Lane 4 : Jurkat cell lysate

    Lysates/proteins at 20 µg per lane.

    Predicted band size: 92 kDa



    This data was developed using the same antibody clone in a different buffer formulation (ab129195).

    Lanes 1 - 4: Merged signal (red and green). Green - ab129195 observed at 110 kDa. Red - loading control, ab8245, observed at 37 kDa.

    ab129195 was shown to specifically react with KMD1 / LSD1 in wild-type HAP1 cells. No band was observed when KMD1 / LSD1 knockout samples were used. Wild-type and KMD1 / LSD1 knockout samples were subjected to SDS-PAGE. ab129195 and ab8245 (loading control to GAPDH) were both diluted 1/10,000 and incubated overnight at 4°C. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1/10,000 dilution for 1 hour at room temperature before imaging.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-KDM1 / LSD1 antibody [EPR6825] - BSA and Azide free (ab224270)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-KDM1 / LSD1 antibody [EPR6825] - BSA and Azide free (ab224270)
    Immunohistochemical staining of paraffin embedded rat kidney with purified ab129195 at a working dilution of 1/50. The secondary antibody used is ab97051, a goat anti-rabbit IgG (H&L) at a dilution of 1/500. The sample is counter-stained with hematoxylin. Antigen retrieval was perfomed using Tris-EDTA buffer, pH 9.0. PBS was used instead of the primary antibody as the negative control, and is shown in the inset.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab129195).

  • Immunocytochemistry/ Immunofluorescence - Anti-KDM1 / LSD1 antibody [EPR6825] - BSA and Azide free (ab224270)
    Immunocytochemistry/ Immunofluorescence - Anti-KDM1 / LSD1 antibody [EPR6825] - BSA and Azide free (ab224270)

    Immunofluorescence staining of HeLa cells with purified ab129195 at a working dilution of 1/100, counter-stained with DAPI. The secondary antibody was Alexa Fluor® 488 goat anti-rabbit (ab150077), used at a dilution of 1/1000. ab7291, a mouse anti-tubulin antibody (1/1000), was used to stain tubulin along with ab150120 (Alexa Fluor® 594 goat anti-mouse, 1/1000), shown in the top right hand panel. The cells were fixed in 4% PFA and permeabilized using 0.1% Triton X 100. The negative controls are shown in bottom middle and right hand panels - for negative control 1, purified ab129195 was used at a dilution of 1/500 followed by an Alexa Fluor® 594 goat anti-mouse antibody (ab150120) at a dilution of 1/500. For negative control 2, ab7291 (mouse anti-tubulin) was used at a dilution of 1/500 followed by an Alexa Fluor® 488 goat anti-rabbit antibody (ab150077) at a dilution of 1/400.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab129195).

  • Flow Cytometry - Anti-KDM1 / LSD1 antibody [EPR6825] - BSA and Azide free (ab224270)
    Flow Cytometry - Anti-KDM1 / LSD1 antibody [EPR6825] - BSA and Azide free (ab224270)

    Flow Cytometry analysis of HeLa (human cervix adenocarcinoma) cells labeling KDM1/LSD1 with purified ab129195 at 1/20 dilution (10ug/mL) (red). Cells were fixed with 4% paraformaldehyde and permeabilised with 90% methanol. A Goat anti rabbit IgG (Alexa Fluor® 488) (1/2000 dilution) was used as the secondary antibody. Rabbit monoclonal IgG (Black) was used as the isotype control, cells without incubation with primary antibody and secondary antibody (Blue) were used as the unlabeled control.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab129195).

  • Immunoprecipitation - Anti-KDM1 / LSD1 antibody [EPR6825] - BSA and Azide free (ab224270)
    Immunoprecipitation - Anti-KDM1 / LSD1 antibody [EPR6825] - BSA and Azide free (ab224270)

    ab129195 (purified) at 1/20 immunoprecipitating KDM1/LSD1 in 10 µg Jurkat cell lysate (Lanes 1 and 2, observed at 110 kDa). Lane 3 - Rabbit monoclonal IgG (ab172730). For western blotting, VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/10,000 dilution. Blocking buffer and concentration: 5% NFDM/TBST Dilution buffer and concentration: 5% NFDM/TBST

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab129195).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-KDM1 / LSD1 antibody [EPR6825] - BSA and Azide free (ab224270)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-KDM1 / LSD1 antibody [EPR6825] - BSA and Azide free (ab224270)
    Immunohistochemical staining of paraffin embedded mouse colon with purified ab129195 at a working dilution of 1/50. The secondary antibody used is ab97051, a goat anti-rabbit IgG (H&L) at a dilution of 1/500. The sample is counter-stained with hematoxylin. Antigen retrieval was perfomed using Tris-EDTA buffer, pH 9.0. PBS was used instead of the primary antibody as the negative control, and is shown in the inset.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab129195).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-KDM1 / LSD1 antibody [EPR6825] - BSA and Azide free (ab224270)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-KDM1 / LSD1 antibody [EPR6825] - BSA and Azide free (ab224270)
    Immunohistochemical staining of paraffin embedded human stomach carcinoma with purified ab129195 at a working dilution of 1/50. The secondary antibody used is ab97051, a goat anti-rabbit IgG (H&L) at a dilution of 1/500. The sample is counter-stained with hematoxylin. Antigen retrieval was perfomed using Tris-EDTA buffer, pH 9.0. PBS was used instead of the primary antibody as the negative control, and is shown in the inset.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab129195).

  • Immunocytochemistry/ Immunofluorescence - Anti-KDM1/LSD1 antibody [EPR6825] - BSA and Azide free (ab224270)
    Immunocytochemistry/ Immunofluorescence - Anti-KDM1/LSD1 antibody [EPR6825] - BSA and Azide free (ab224270)This image is courtesy of an anonymous Abreview.

    Unpurified ab129195 staining KDM1/LSD1 in human paraffin-embedded A549 lung cancer cells by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed using the HOPE technique and permeabilized with 0.05% Tween. Samples were incubated with primary antibody (1/100) for 45 minutes at 25°C. An Alexa Fluor®488-conjugated Donkey anti-mouse IgG polyclonal (1/200) was used as the secondary antibody.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab129195).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-KDM1 / LSD1 antibody [EPR6825] - BSA and Azide free (ab224270)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-KDM1 / LSD1 antibody [EPR6825] - BSA and Azide free (ab224270)

    Unpurified ab129195, at 1/100, staining KDM1 / LSD1 in paraffin embedded Human testis tissue by Immunohistochemistry.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab129195).

    Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

  • Anti-KDM1/LSD1 antibody [EPR6825] - BSA and Azide free (ab224270)
    Anti-KDM1/LSD1 antibody [EPR6825] - BSA and Azide free (ab224270)

Protocols

  • Flow cytometry protocols
  • Immunoprecipitation protocols
  • Immunohistochemistry protocols
  • Immunocytochemistry & immunofluorescence protocols
  • Western blot protocols

Click here to view the general protocols

Datasheets and documents

    • Datasheet

    Certificate of Compliance

    To download a Certificate of Compliance, please enter your Lot number below:

  • References (3)

    Publishing research using ab224270? Please let us know so that we can cite the reference in this datasheet.

    ab224270 has been referenced in 3 publications.

    • Kilinc S  et al. Lysine-specific demethylase-1 (LSD1) is compartmentalized at nuclear chromocenters in early post-mitotic cells of the olfactory sensory neuronal lineage. Mol Cell Neurosci 74:58-70 (2016). WB, IP, IF, IHC ; Mouse . PubMed: 26947098
    • Xiong Y  et al. Inhibition of Lysine-Specific Demethylase-1 (LSD1/KDM1A) Promotes the Adipogenic Differentiation of hESCs Through H3K4 Methylation. Stem Cell Rev : (2016). WB . PubMed: 27059868
    • Mould AW  et al. Blimp1/Prdm1 Functions in Opposition to Irf1 to Maintain Neonatal Tolerance during Postnatal Intestinal Maturation. PLoS Genet 11:e1005375 (2015). WB . PubMed: 26158850

    Customer reviews and Q&As

    Show All Reviews Q&A
    Submit a review Submit a question

    There are currently no Customer reviews or Questions for ab224270.
    Please use the links above to contact us or submit feedback about this product.

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
    For licensing inquiries, please contact partnerships@abcam.com

    Get resources and offers direct to your inbox Sign up
    A-Z by research area
    • Cancer
    • Cardiovascular
    • Cell biology
    • Developmental biology
    • Epigenetics & Nuclear signaling
    • Immunology
    • Metabolism
    • Microbiology
    • Neuroscience
    • Signal transduction
    • Stem cells
    A-Z by product type
    • Primary antibodies
    • Secondary antibodies
    • Biochemicals
    • Isotype controls
    • Flow cytometry multi-color selector
    • Kits
    • Loading controls
    • Lysates
    • Peptides
    • Proteins
    • Slides
    • Tags and cell markers
    • Tools & Reagents
    Help & support
    • Support
    • Make an Inquiry
    • Protocols & troubleshooting
    • Placing an order
    • RabMAb products
    • Biochemical product FAQs
    • Training
    • Browse by Target
    Company
    • Corporate site
    • Investor relations
    • Company news
    • Careers
    • About us
    • Blog
    Events
    • Tradeshows
    • Conferences
    International websites
    • abcam.cn
    • abcam.co.jp

    Join with us

    • LinkedIn
    • facebook
    • Twitter
    • YouTube
    • Terms of sale
    • Website terms of use
    • Cookie policy
    • Privacy policy
    • Legal
    • Modern slavery statement
    © 1998-2021 Abcam plc. All rights reserved.