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  1. Link

    kdm1lsd1-antibody-nuclear-marker-ab17721.pdf

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Epigenetics and Nuclear Signaling Chromatin Modifying Enzymes Methylation Lysine demethylation
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Validated using a knockout cell line

Anti-KDM1/LSD1 antibody - Nuclear Marker (ab17721)

  • Datasheet
  • SDS
Reviews (24)Q&A (27)References (199)

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Western blot - Anti-KDM1/LSD1 antibody - Nuclear Marker (ab17721)
  • Immunocytochemistry - Anti-KDM1/LSD1 antibody - Nuclear Marker (ab17721)
  • Western blot - Anti-KDM1/LSD1 antibody - Nuclear Marker (ab17721)
  • Western blot - Anti-KDM1/LSD1 antibody - Nuclear Marker and ChIP Grade (ab17721)
  • Immunocytochemistry/ Immunofluorescence - Anti-KDM1/LSD1 antibody - Nuclear Marker and ChIP Grade (ab17721)
  • Immunocytochemistry/ Immunofluorescence - Anti-KDM1/LSD1 antibody - Nuclear Marker and ChIP Grade (ab17721)

Key features and details

  • Rabbit polyclonal to KDM1/LSD1 - Nuclear Marker
  • Suitable for: ICC/IF, WB
  • Knockout validated
  • Reacts with: Mouse, Rat, Human
  • Isotype: IgG

Get better batch-to-batch reproducibility with a recombinant antibody

Product image
Anti-KDM1/LSD1 antibody [EPR6825] - Nuclear Marker and ChIP Grade (ab129195)
  • Research with confidence – consistent and reproducible results with every batch
  • Long-term and scalable supply – powered by recombinant technology for fast production
  • Success from the first experiment – confirmed specificity through extensive validation
  • Ethical standards compliant – production is animal-free

Overview

  • Product name

    Anti-KDM1/LSD1 antibody - Nuclear Marker
    See all KDM1/LSD1 primary antibodies
  • Description

    Rabbit polyclonal to KDM1/LSD1 - Nuclear Marker
  • Host species

    Rabbit
  • Tested applications

    Suitable for: ICC/IF, WBmore details
  • Species reactivity

    Reacts with: Mouse, Rat, Human
    Predicted to work with: Pig
  • Immunogen

    Synthetic peptide conjugated to KLH derived from within residues 800 to the C-terminus of Human LSD1.

    Read Abcam's proprietary immunogen policy (Peptide available as ab17763.)
  • Positive control

    • ICC/IF: HeLa, 293T, c2c12 cells. WB: Jurkat, HCT 116, NIH/3T3, C2C12, Mouse skeletal muscle, C6, HeLa.
  • General notes

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.40
    Preservative: 0.02% Sodium azide
    Constituent: PBS

    Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help.
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

    • Epigenetics and Nuclear Signaling
    • Chromatin Modifying Enzymes
    • Methylation
    • Lysine demethylation
    • Neuroscience
    • Processes

Associated products

  • Assay kits

    • Histone Demethylase KDM1/LSD1 Inhibitor Assay Kit (ab113456)
    • KDM1/LSD1 Activity Quantification Assay Kit (Colorimetric) (ab113459)
  • ChIP Related Products

    • Rabbit Anti-Mouse IgG H&L (ab46540)
    • ChIP Kit (ab500)
  • Compatible Secondaries

    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
    • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
  • Immunizing Peptide (Blocking)

    • Human KDM1/LSD1 peptide (ab17763)
  • Isotype control

    • Rabbit IgG, polyclonal - Isotype Control (ChIP Grade) (ab171870)
  • Related Products

    • Prestained Protein Ladder – Broad molecular weight (10-245 kDa) (ab116028)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab17721 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF (3)
Use a concentration of 1 µg/ml.
WB (12)
Use a concentration of 1 µg/ml. Detects a band of approximately 105 kDa (predicted molecular weight: 93 kDa).Can be blocked with Human KDM1/LSD1 peptide (ab17763).
Notes
ICC/IF
Use a concentration of 1 µg/ml.
WB
Use a concentration of 1 µg/ml. Detects a band of approximately 105 kDa (predicted molecular weight: 93 kDa).Can be blocked with Human KDM1/LSD1 peptide (ab17763).

Target

  • Function

    Histone demethylase that demethylates both 'Lys-4' (H3K4me) and 'Lys-9' (H3K9me) of histone H3, thereby acting as a coactivator or a corepressor, depending on the context. Acts by oxidizing the substrate by FAD to generate the corresponding imine that is subsequently hydrolyzed. Acts as a corepressor by mediating demethylation of H3K4me, a specific tag for epigenetic transcriptional activation. Demethylates both mono- (H3K4me1) and di-methylated (H3K4me2) H3K4me. May play a role in the repression of neuronal genes. Alone, it is unable to demethylate H3K4me on nucleosomes and requires the presence of RCOR1/CoREST to achieve such activity. Also acts as a coactivator of androgen receptor (ANDR)-dependent transcription, by being recruited to ANDR target genes and mediating demethylation of H3K9me, a specific tag for epigenetic transcriptional repression. The presence of PRKCB in ANDR-containing complexes, which mediates phosphorylation of 'Thr-6' of histone H3 (H3T6ph), a specific tag that prevents demethylation H3K4me, prevents H3K4me demethylase activity of KDM1A. Demethylates di-methylated 'Lys-370' of p53/TP53 which prevents interaction of p53/TP53 with TP53BP1 and represses p53/TP53-mediated transcriptional activation. Demethylates and stabilizes the DNA methylase DNMT1. Required for gastrulation during embryogenesis.
  • Tissue specificity

    Ubiquitously expressed.
  • Sequence similarities

    Belongs to the flavin monoamine oxidase family.
    Contains 1 SWIRM domain.
  • Domain

    The SWIRM domain may act as an anchor site for a histone tail.
  • Cellular localization

    Nucleus.
  • Target information above from: UniProt accession O60341 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 23028 Human
    • Entrez Gene: 99982 Mouse
    • Entrez Gene: 500569 Rat
    • Omim: 609132 Human
    • SwissProt: O60341 Human
    • SwissProt: Q6ZQ88 Mouse
    • Unigene: 591518 Human
    • Unigene: 28540 Mouse
    • Alternative names

      • Amine oxidase (flavin containing) domain 2 antibody
      • Amine oxidase, flavin containing, 2 antibody
      • AOF2 antibody
      • BHC110 antibody
      • BRAF35 HDAC complex protein BHC110 antibody
      • BRAF35-HDAC complex protein BHC110 antibody
      • BRAF35/HDAC complex, 110-kD subunit antibody
      • CPRF antibody
      • EC1 antibody
      • FAD binding protein BRAF35 HDAC complex, 110 kDa subunit antibody
      • Flavin-containing amine oxidase domain-containing protein 2 antibody
      • KDM 1 antibody
      • KDM1 antibody
      • Kdm1a antibody
      • KDM1A_HUMAN antibody
      • KIAA0601 antibody
      • LSD 1 antibody
      • LSD1 antibody
      • Lysine (K) specific demethylase 1 antibody
      • Lysine (K) specific demethylase 1A antibody
      • Lysine demethylase 1A antibody
      • Lysine specific histone demethylase 1 antibody
      • Lysine specific histone demethylase 1A antibody
      • Lysine-specific demethylase 1 antibody
      • Lysine-specific demethylase 1A antibody
      • Lysine-specific histone demethylase 1A antibody
      see all

    Images

    • Western blot - Anti-KDM1/LSD1 antibody - Nuclear Marker (ab17721)
      Western blot - Anti-KDM1/LSD1 antibody - Nuclear Marker (ab17721)

      Lane 1: Wild type HAP1 whole cell lysate (20 µg)
      Lane 2: KDM1 / LSD1 knockout HAP1 whole cell lysate (20 µg)
      Lane 3: HeLa whole cell lysate (20 µg)
      Lane 4: Jurkat whole cell lysate (20 µg)
      Lanes 1 - 4: Merged signal (red and green). Green - ab17721 observed at 110 kDa. Red - loading control, ab8245, observed at 37 kDa.

      ab17721 was shown to specifically react with KDMI/LSD1 in wild-type HAP1 cells. No band was observed when KDMI/LSD1 knockout samples were examined.

    • Immunocytochemistry - Anti-KDM1/LSD1 antibody - Nuclear Marker (ab17721)
      Immunocytochemistry - Anti-KDM1/LSD1 antibody - Nuclear Marker (ab17721)

      ab17721 staining KDM1 / LSD1 in HeLa cells. The cells were fixed with 100% methanol (5 min), permeabilized with 0.1% PBS-Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated overnight at 4°C with ab17721 at 1µg/ml and ab7291, Mouse monoclonal [DM1A] to alpha Tubulin - Loading Control. Cells were then incubated with ab150081, Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (Alexa Fluor® 488), pre-adsorbed at 1/1000 dilution (shown in green) and ab150120, Goat polyclonal Secondary Antibody to Mouse IgG - H&L (Alexa Fluor® 594), pre-adsorbed at 1/1000 dilution (shown in pseudocolour red). Nuclear DNA was labelled with DAPI (shown in blue).

      Also suitable in cells fixed with 4% paraformaldehyde (10 min).

      Image was acquired with a high-content analyser (Operetta CLS, Perkin Elmer) and a maximum intensity projection of confocal sections is shown.

    • Western blot - Anti-KDM1/LSD1 antibody - Nuclear Marker (ab17721)
      Western blot - Anti-KDM1/LSD1 antibody - Nuclear Marker (ab17721)
      All lanes :

      Lane 1 : Jurkat (Human T cell leukemia T lymphocyte) whole cell lysates at 20 µg
      Lane 2 : HCT 116 (Human colorectal carcinoma epithelial cell) whole cell lysates at 20 µg
      Lane 3 : NIH/3T3 (Mouse embryonic fibroblast) whole cell lysates at 20 µg
      Lane 4 : C2C12 (Mouse myoblasts myoblast) whole cell lysates
      Lane 5 : Mouse skeletal muscle lysates at 20 µg
      Lane 6 : C6 (Rat glial tumor glial cell) whole cell lysates at 20 µg

      Secondary
      All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (ab97051) at 1/20000 dilution

      Predicted band size: 93 kDa


      Exposure time: 50 seconds


      Observed MW: 110kd

      Blocking Buffer & Diluting buffer concentration: 5% NFDM/TBST

    • Western blot - Anti-KDM1/LSD1 antibody - Nuclear Marker and ChIP Grade (ab17721)
      Western blot - Anti-KDM1/LSD1 antibody - Nuclear Marker and ChIP Grade (ab17721)
      All lanes : Anti-KDM1/LSD1 antibody - Nuclear Marker (ab17721) at 1 µg/ml

      Lane 1 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
      Lane 2 : Jurkat whole cell lysate (ab7899)
      Lane 3 : NIH/3T3 whole cell lysate (ab7179)

      Lysates/proteins at 20 µg per lane.

      Secondary
      All lanes : Goat polyclonal to Rabbit IgG H&L (HRP) Pre-Adsorbed at 1/10000 dilution

      Performed under reducing conditions.

      Predicted band size: 93 kDa

    • Immunocytochemistry/ Immunofluorescence - Anti-KDM1/LSD1 antibody - Nuclear Marker and ChIP Grade (ab17721)
      Immunocytochemistry/ Immunofluorescence - Anti-KDM1/LSD1 antibody - Nuclear Marker and ChIP Grade (ab17721)Image courtesy of anonymous abreviewer

      ab17721 staining LSD1 in the mouse c2c12 cell line by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with paraformaldehyde, permeabilized with 0.5% Triton X-100 and blocked with 2% BSA for 1 hour at 25°C. Samples were incubated with primary antibody (1/200) for 1 hour at 25°C. A diluted (1/2000) Alexa Fluor® 568-conjugated Goat anti-rabbit IgG polyclonal was used as the secondary antibody.

      See Abreview

    • Immunocytochemistry/ Immunofluorescence - Anti-KDM1/LSD1 antibody - Nuclear Marker and ChIP Grade (ab17721)
      Immunocytochemistry/ Immunofluorescence - Anti-KDM1/LSD1 antibody - Nuclear Marker and ChIP Grade (ab17721)This image is courtesy of Katia Ancelin
      Human 293T cells fixed in 4% paraformaldehyde were immunostained with ab17721 (1/200) for LSD1 and detected using FITC labelled anti-rabbit (Green). Nuclear DNA is stained blue with DAPI.

    Protocols

    • Immunocytochemistry & immunofluorescence protocols
    • Western blot protocols

    Click here to view the general protocols

    Datasheets and documents

    • SDS download

    • Datasheet download

      Download

    References (199)

    Publishing research using ab17721? Please let us know so that we can cite the reference in this datasheet.

    ab17721 has been referenced in 199 publications.

    • Guénantin AC  et al. Targeting the histone demethylase LSD1 prevents cardiomyopathy in a mouse model of laminopathy. J Clin Invest 131:N/A (2021). PubMed: 33393499
    • Noches V  et al. Pilocarpine-induced seizures associate with modifications of LSD1/CoREST/HDAC1/2 epigenetic complex and repressive chromatin in mice hippocampus. Biochem Biophys Rep 25:100889 (2021). PubMed: 33426312
    • Mak KH  et al. Histone demethylase JMJD2B/KDM4B regulates transcriptional program via distinctive epigenetic targets and protein interactors for the maintenance of trophoblast stem cells. Sci Rep 11:884 (2021). PubMed: 33441614
    • Rong J  et al. microRNA-362-3p targets USP22 to retard retinoblastoma growth via reducing deubiquitination of LSD1. Cell Cycle 20:298-307 (2021). PubMed: 33475455
    • Pinter S  et al. A functional LSD1 coregulator screen reveals a novel transcriptional regulatory cascade connecting R-loop homeostasis with epigenetic regulation. Nucleic Acids Res 49:4350-4370 (2021). PubMed: 33823549
    View all Publications for this product

    Customer reviews and Q&As

    Show All Reviews Q&A
    Submit a review Submit a question

    1-10 of 51 Abreviews or Q&A

    ChIP abreview for Anti-KDM1/LSD1 antibody - Nuclear Marker

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    ChIP
    Sample
    Mouse Cell lysate - nuclear (hippocampus)
    Negative control
    normal rabbit IgG sc-3888
    Specification
    hippocampus
    Detection step
    Real-time PCR
    Type
    Cross-linking (X-ChIP)
    Duration of cross-linking step: 15 minute(s) and 0 second(s)
    Specification of the cross-linking agent: 1% Formaldehyde in PBS
    Positive control
    Active gene Actb and repressed gene Hoxa1
    Read More

    Abcam user community

    Verified customer

    Submitted Aug 13 2020

    Immunocytochemistry/ Immunofluorescence abreview for Anti-KDM1/LSD1 antibody - Nuclear Marker and ChIP Grade

    Excellent
    Abreviews
    Abreviews
    Application
    Immunocytochemistry/ Immunofluorescence
    Sample
    Human Cell (Trophoblast cells)
    Permeabilization
    Yes - 0.1% TRITON
    Specification
    Trophoblast cells
    Blocking step
    Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 27°C
    Fixative
    Paraformaldehyde
    Read More

    Abcam user community

    Verified customer

    Submitted Oct 25 2019

    Western blot abreview for Anti-KDM1/LSD1 antibody - Nuclear Marker and ChIP Grade

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Human Cell lysate - whole cell (HCT116)
    Gel Running Conditions
    Reduced Denaturing (7.5%)
    Loading amount
    100000 cells
    Specification
    HCT116
    Blocking step
    Milk as blocking agent for 30 minute(s) · Concentration: 3% · Temperature: 25°C
    Read More

    Abcam user community

    Verified customer

    Submitted Sep 03 2019

    Western blot abreview for Anti-KDM1/LSD1 antibody - Nuclear Marker and ChIP Grade

    Good
    Abreviews
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Mouse Cell lysate - whole cell (Mouse Embryonic Fibroblast)
    Gel Running Conditions
    Reduced Denaturing (4-12% Bis-Tris)
    Loading amount
    20 µg
    Specification
    Mouse Embryonic Fibroblast
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
    Read More

    Abcam user community

    Verified customer

    Submitted Oct 10 2018

    Western blot abreview for Anti-KDM1/LSD1 antibody - Nuclear Marker and ChIP Grade

    Good
    Abreviews
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Human Cell lysate - whole cell (HEK293T)
    Gel Running Conditions
    Reduced Denaturing (4-12% Bis-Tris)
    Loading amount
    20 µg
    Specification
    HEK293T
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
    Read More

    Abcam user community

    Verified customer

    Submitted Oct 10 2018

    Western blot abreview for Anti-KDM1/LSD1 antibody - Nuclear Marker and ChIP Grade

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Human Cell lysate - whole cell (293T)
    Gel Running Conditions
    Reduced Denaturing (3-8)
    Loading amount
    1 µg
    Specification
    293T
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 21°C
    Read More

    Abcam user community

    Verified customer

    Submitted Oct 02 2017

    Immunocytochemistry/ Immunofluorescence abreview for Anti-KDM1/LSD1 antibody - Nuclear Marker and ChIP Grade

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Immunocytochemistry/ Immunofluorescence
    Sample
    Human Cell (HeLa cells)
    Permeabilization
    Yes - 0.1% TX100, 2 mins
    Specification
    HeLa cells
    Blocking step
    BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 21°C
    Fixative
    Paraformaldehyde
    Read More

    Abcam user community

    Verified customer

    Submitted Oct 02 2017

    Immunoprecipitation abreview for Anti-KDM1/LSD1 antibody - Nuclear Marker and ChIP Grade

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Immunoprecipitation
    Sample
    Mouse Tissue lysate - whole (postnatal day 1 mouse brain)
    Total protein in input
    500 µg
    Immuno-precipitation step
    Protein G
    Specification
    postnatal day 1 mouse brain
    Read More

    Abcam user community

    Verified customer

    Submitted Mar 15 2017

    Western blot abreview for Anti-KDM1/LSD1 antibody - Nuclear Marker and ChIP Grade

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Mouse Cell lysate - whole cell (mouse embryonic stem cells)
    Gel Running Conditions
    Reduced Denaturing (10)
    Loading amount
    15 µg
    Specification
    mouse embryonic stem cells
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 21°C
    Read More

    Abcam user community

    Verified customer

    Submitted Jan 25 2016

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-KDM1/LSD1 antibody - Nuclear Marker and ChIP Grade

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample
    Mouse Tissue sections (Brain)
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: Sodium Citrate
    Permeabilization
    Yes - Brij
    Specification
    Brain
    Blocking step
    Serum as blocking agent for 30 minute(s) · Concentration: 0.2% · Temperature: 37°C
    Fixative
    Paraformaldehyde
    Read More

    Abcam user community

    Verified customer

    Submitted Aug 21 2015

    1-10 of 51 Abreviews or Q&A

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