Anti-KDM1/LSD1 antibody - Nuclear Marker (ab17721)
Key features and details
- Rabbit polyclonal to KDM1/LSD1 - Nuclear Marker
- Suitable for: ICC/IF, WB
- Knockout validated
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
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- Research with confidence – consistent and reproducible results with every batch
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- Success from the first experiment – confirmed specificity through extensive validation
- Ethical standards compliant – production is animal-free
Overview
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Product name
Anti-KDM1/LSD1 antibody - Nuclear Marker
See all KDM1/LSD1 primary antibodies -
Description
Rabbit polyclonal to KDM1/LSD1 - Nuclear Marker -
Host species
Rabbit -
Tested applications
Suitable for: ICC/IF, WBmore details -
Species reactivity
Reacts with: Mouse, Rat, Human
Predicted to work with: Pig
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Immunogen
Synthetic peptide conjugated to KLH derived from within residues 800 to the C-terminus of Human LSD1.
Read Abcam's proprietary immunogen policy (Peptide available as ab17763.) -
Positive control
- ICC/IF: HeLa, 293T, c2c12 cells. WB: Jurkat, HCT 116, NIH/3T3, C2C12, Mouse skeletal muscle, C6, HeLa.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituent: PBS
Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help. -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Assay kits
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ChIP Related Products
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Compatible Secondaries
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Immunizing Peptide (Blocking)
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Isotype control
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Related Products
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab17721 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| ICC/IF | (3) |
Use a concentration of 1 µg/ml.
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| WB | (12) |
Use a concentration of 1 µg/ml. Detects a band of approximately 105 kDa (predicted molecular weight: 93 kDa).Can be blocked with Human KDM1/LSD1 peptide (ab17763).
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| Notes |
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ICC/IF
Use a concentration of 1 µg/ml. |
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WB
Use a concentration of 1 µg/ml. Detects a band of approximately 105 kDa (predicted molecular weight: 93 kDa).Can be blocked with Human KDM1/LSD1 peptide (ab17763). |
Target
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Function
Histone demethylase that demethylates both 'Lys-4' (H3K4me) and 'Lys-9' (H3K9me) of histone H3, thereby acting as a coactivator or a corepressor, depending on the context. Acts by oxidizing the substrate by FAD to generate the corresponding imine that is subsequently hydrolyzed. Acts as a corepressor by mediating demethylation of H3K4me, a specific tag for epigenetic transcriptional activation. Demethylates both mono- (H3K4me1) and di-methylated (H3K4me2) H3K4me. May play a role in the repression of neuronal genes. Alone, it is unable to demethylate H3K4me on nucleosomes and requires the presence of RCOR1/CoREST to achieve such activity. Also acts as a coactivator of androgen receptor (ANDR)-dependent transcription, by being recruited to ANDR target genes and mediating demethylation of H3K9me, a specific tag for epigenetic transcriptional repression. The presence of PRKCB in ANDR-containing complexes, which mediates phosphorylation of 'Thr-6' of histone H3 (H3T6ph), a specific tag that prevents demethylation H3K4me, prevents H3K4me demethylase activity of KDM1A. Demethylates di-methylated 'Lys-370' of p53/TP53 which prevents interaction of p53/TP53 with TP53BP1 and represses p53/TP53-mediated transcriptional activation. Demethylates and stabilizes the DNA methylase DNMT1. Required for gastrulation during embryogenesis. -
Tissue specificity
Ubiquitously expressed. -
Sequence similarities
Belongs to the flavin monoamine oxidase family.
Contains 1 SWIRM domain. -
Domain
The SWIRM domain may act as an anchor site for a histone tail. -
Cellular localization
Nucleus. - Information by UniProt
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Database links
- Entrez Gene: 23028 Human
- Entrez Gene: 99982 Mouse
- Entrez Gene: 500569 Rat
- Omim: 609132 Human
- SwissProt: O60341 Human
- SwissProt: Q6ZQ88 Mouse
- Unigene: 591518 Human
- Unigene: 28540 Mouse
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Alternative names
- Amine oxidase (flavin containing) domain 2 antibody
- Amine oxidase, flavin containing, 2 antibody
- AOF2 antibody
see all
Images
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Western blot - Anti-KDM1/LSD1 antibody - Nuclear Marker (ab17721)
Lane 1: Wild type HAP1 whole cell lysate (20 µg)
Lane 2: KDM1 / LSD1 knockout HAP1 whole cell lysate (20 µg)
Lane 3: HeLa whole cell lysate (20 µg)
Lane 4: Jurkat whole cell lysate (20 µg)
Lanes 1 - 4: Merged signal (red and green). Green - ab17721 observed at 110 kDa. Red - loading control, ab8245, observed at 37 kDa.ab17721 was shown to specifically react with KDMI/LSD1 in wild-type HAP1 cells. No band was observed when KDMI/LSD1 knockout samples were examined.
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Immunocytochemistry - Anti-KDM1/LSD1 antibody - Nuclear Marker (ab17721)ab17721 staining KDM1 / LSD1 in HeLa cells. The cells were fixed with 100% methanol (5 min), permeabilized with 0.1% PBS-Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated overnight at 4°C with ab17721 at 1µg/ml and ab7291, Mouse monoclonal [DM1A] to alpha Tubulin - Loading Control. Cells were then incubated with ab150081, Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (Alexa Fluor® 488), pre-adsorbed at 1/1000 dilution (shown in green) and ab150120, Goat polyclonal Secondary Antibody to Mouse IgG - H&L (Alexa Fluor® 594), pre-adsorbed at 1/1000 dilution (shown in pseudocolour red). Nuclear DNA was labelled with DAPI (shown in blue).
Also suitable in cells fixed with 4% paraformaldehyde (10 min).
Image was acquired with a high-content analyser (Operetta CLS, Perkin Elmer) and a maximum intensity projection of confocal sections is shown.
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Western blot - Anti-KDM1/LSD1 antibody - Nuclear Marker (ab17721)All lanes :
Lane 1 : Jurkat (Human T cell leukemia T lymphocyte) whole cell lysates at 20 µg
Lane 2 : HCT 116 (Human colorectal carcinoma epithelial cell) whole cell lysates at 20 µg
Lane 3 : NIH/3T3 (Mouse embryonic fibroblast) whole cell lysates at 20 µg
Lane 4 : C2C12 (Mouse myoblasts myoblast) whole cell lysates
Lane 5 : Mouse skeletal muscle lysates at 20 µg
Lane 6 : C6 (Rat glial tumor glial cell) whole cell lysates at 20 µg
Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (ab97051) at 1/20000 dilution
Predicted band size: 93 kDa
Exposure time: 50 secondsObserved MW: 110kd
Blocking Buffer & Diluting buffer concentration: 5% NFDM/TBST
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Western blot - Anti-KDM1/LSD1 antibody - Nuclear Marker and ChIP Grade (ab17721)All lanes : Anti-KDM1/LSD1 antibody - Nuclear Marker (ab17721) at 1 µg/ml
Lane 1 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
Lane 2 :Jurkat whole cell lysate (ab7899)
Lane 3 :NIH/3T3 whole cell lysate (ab7179)
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat polyclonal to Rabbit IgG H&L (HRP) Pre-Adsorbed at 1/10000 dilution
Performed under reducing conditions.
Predicted band size: 93 kDa -
Immunocytochemistry/ Immunofluorescence - Anti-KDM1/LSD1 antibody - Nuclear Marker and ChIP Grade (ab17721)Image courtesy of anonymous abreviewerab17721 staining LSD1 in the mouse c2c12 cell line by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with paraformaldehyde, permeabilized with 0.5% Triton X-100 and blocked with 2% BSA for 1 hour at 25°C. Samples were incubated with primary antibody (1/200) for 1 hour at 25°C. A diluted (1/2000) Alexa Fluor® 568-conjugated Goat anti-rabbit IgG polyclonal was used as the secondary antibody.
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Immunocytochemistry/ Immunofluorescence - Anti-KDM1/LSD1 antibody - Nuclear Marker and ChIP Grade (ab17721)This image is courtesy of Katia AncelinHuman 293T cells fixed in 4% paraformaldehyde were immunostained with ab17721 (1/200) for LSD1 and detected using FITC labelled anti-rabbit (Green). Nuclear DNA is stained blue with DAPI.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (199)
ab17721 has been referenced in 199 publications.
- Guénantin AC et al. Targeting the histone demethylase LSD1 prevents cardiomyopathy in a mouse model of laminopathy. J Clin Invest 131:N/A (2021). PubMed: 33393499
- Noches V et al. Pilocarpine-induced seizures associate with modifications of LSD1/CoREST/HDAC1/2 epigenetic complex and repressive chromatin in mice hippocampus. Biochem Biophys Rep 25:100889 (2021). PubMed: 33426312
- Mak KH et al. Histone demethylase JMJD2B/KDM4B regulates transcriptional program via distinctive epigenetic targets and protein interactors for the maintenance of trophoblast stem cells. Sci Rep 11:884 (2021). PubMed: 33441614
- Rong J et al. microRNA-362-3p targets USP22 to retard retinoblastoma growth via reducing deubiquitination of LSD1. Cell Cycle 20:298-307 (2021). PubMed: 33475455
- Pinter S et al. A functional LSD1 coregulator screen reveals a novel transcriptional regulatory cascade connecting R-loop homeostasis with epigenetic regulation. Nucleic Acids Res 49:4350-4370 (2021). PubMed: 33823549