Anti-KDM1/LSD1 antibody - Nuclear Marker (ab37165)
Key features and details
- Rabbit polyclonal to KDM1/LSD1 - Nuclear Marker
- Suitable for: IHC-P, WB
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
Overview
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Product name
Anti-KDM1/LSD1 antibody - Nuclear Marker
See all KDM1/LSD1 primary antibodies -
Description
Rabbit polyclonal to KDM1/LSD1 - Nuclear Marker -
Host species
Rabbit -
Tested applications
Suitable for: IHC-P, WBmore details -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Synthetic peptide within Human KDM1/LSD1 (N terminal). The exact sequence is proprietary. ab37165 was raised against a peptide corresponding to 17 amino acids near the N terminus of human LSD1.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.02% Sodium azide
Constituent: PBS -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Assay kits
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Compatible Secondaries
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Isotype control
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Positive Controls
Applications
Our Abpromise guarantee covers the use of ab37165 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| IHC-P | Use at an assay dependent concentration. | |
| WB | Use a concentration of 1 - 2 µg/ml. Detects a band of approximately 105 kDa (predicted molecular weight: 93 kDa). |
Target
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Function
Histone demethylase that demethylates both 'Lys-4' (H3K4me) and 'Lys-9' (H3K9me) of histone H3, thereby acting as a coactivator or a corepressor, depending on the context. Acts by oxidizing the substrate by FAD to generate the corresponding imine that is subsequently hydrolyzed. Acts as a corepressor by mediating demethylation of H3K4me, a specific tag for epigenetic transcriptional activation. Demethylates both mono- (H3K4me1) and di-methylated (H3K4me2) H3K4me. May play a role in the repression of neuronal genes. Alone, it is unable to demethylate H3K4me on nucleosomes and requires the presence of RCOR1/CoREST to achieve such activity. Also acts as a coactivator of androgen receptor (ANDR)-dependent transcription, by being recruited to ANDR target genes and mediating demethylation of H3K9me, a specific tag for epigenetic transcriptional repression. The presence of PRKCB in ANDR-containing complexes, which mediates phosphorylation of 'Thr-6' of histone H3 (H3T6ph), a specific tag that prevents demethylation H3K4me, prevents H3K4me demethylase activity of KDM1A. Demethylates di-methylated 'Lys-370' of p53/TP53 which prevents interaction of p53/TP53 with TP53BP1 and represses p53/TP53-mediated transcriptional activation. Demethylates and stabilizes the DNA methylase DNMT1. Required for gastrulation during embryogenesis. -
Tissue specificity
Ubiquitously expressed. -
Sequence similarities
Belongs to the flavin monoamine oxidase family.
Contains 1 SWIRM domain. -
Domain
The SWIRM domain may act as an anchor site for a histone tail. -
Cellular localization
Nucleus. - Information by UniProt
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Database links
- Entrez Gene: 23028 Human
- Entrez Gene: 99982 Mouse
- Entrez Gene: 500569 Rat
- Omim: 609132 Human
- SwissProt: O60341 Human
- SwissProt: Q6ZQ88 Mouse
- Unigene: 591518 Human
- Unigene: 28540 Mouse
see all -
Alternative names
- Amine oxidase (flavin containing) domain 2 antibody
- Amine oxidase, flavin containing, 2 antibody
- AOF2 antibody
see all
Images
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Western blot - Anti-KDM1/LSD1 antibody - Nuclear Marker (ab37165)All lanes : Anti-KDM1/LSD1 antibody - Nuclear Marker (ab37165) at 0.2 µg/ml
Lane 1 : HEK-293 (human epithelial cell line from embryonic kidney) cell lysate
Lane 2 : A431 (human epidermoid carcinoma cell line) cell lysate
Lane 3 : A549 (human lung carcinoma cell line) cell lysate
Lane 4 : Caco-2 (human colorectal adenocarcinoma cell line) cell lysate
Lane 5 : Daudi (human Burkitt's lymphoma cell line) cell lysate
Lane 6 : HeLa (human epithelial cell line from cervix adenocarcinoma) cell lysate
Lane 7 : HepG2 (human liver hepatocellular carcinoma cell line) cell lysate
Lane 8 : K562 (human chronic myelogenous leukemia cell line from bone marrow) cell lysate
Lane 9 : MCF7 (human breast adenocarcinoma cell line) cell lysate
Lane 10 : Jurkat (human T cell leukemia cell line from peripheral blood) cell lysate
Lane 11 : SK-N-SH (human neuroblastoma cell line) cell lysate
Lane 12 : THP-1 (human monocytic leukemia cell line) cell lysate
Lane 13 : NIH/3T3 (mouse embryo fibroblast cell line) cell lysate
Lane 14 : YB2/0 (rat spleen lymphoblast cell line) cell lysate
Lysates/proteins at 15 µg per lane.
Secondary
All lanes : Goat anti-rabbit IgG (HRP) at 1/10000 dilution
Predicted band size: 93 kDa
Observed band size: 110 kDa why is the actual band size different from the predicted?Diluting buffer and concentration: 1 hour at room temperature in 5% NFDM/TBST.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-KDM1/LSD1 antibody - Nuclear Marker (ab37165)IHC image of KDM1/LSD1 staining in human prostate formalin fixed paraffin embedded tissue section, performed on a Leica Bond system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab37165, 1µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
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Western blot - Anti-KDM1/LSD1 antibody - Nuclear Marker (ab37165)All lanes : Anti-KDM1/LSD1 antibody - Nuclear Marker (ab37165) at 0.2 µg/ml
Lane 1 : Mouse Testis tissue lysate
Lane 2 : Mouse Spleen tissue lysate
Lane 3 : Mouse Kidney tissue lysate
Lane 4 : Mouse Colon tissue lysate
Lane 5 : Mouse Stomach tissue lysate
Lane 6 : Mouse Brain tissue lysate
Lane 7 : Mouse Skin tissue lysate
Lane 8 : Mouse Skeletal Muscle tissue lysate
Lane 9 : Mouse Bladder tissue lysate
Lysates/proteins at 15 µg per lane.
Secondary
All lanes : Goat anti-rabbit IgG (HRP) at 1/10000 dilution
Predicted band size: 93 kDa
Observed band size: 110 kDa why is the actual band size different from the predicted?Diluting buffer and concentration: 1 hour at room temperature in 5% NFDM/TBST.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-KDM1/LSD1 antibody - Nuclear Marker (ab37165)Immunohistochemistry (Formalin-fixed paraffin embedded sections) of human breast carcinoma tissue labeling KDM1/LSD1 with ab37165 at 2μg/ml.
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Western blot - Anti-KDM1/LSD1 antibody - Nuclear Marker (ab37165)Lane 1 : Anti-KDM1/LSD1 antibody - Nuclear Marker (ab37165) at 1 µg/ml
Lane 2 : Anti-KDM1/LSD1 antibody - Nuclear Marker (ab37165) at 2 µg/ml
All lanes : Mouse P815 cell lysate
Lysates/proteins at 15 µg per lane.
Predicted band size: 93 kDa
Observed band size: 105 kDa why is the actual band size different from the predicted?
Protocols
Datasheets and documents
References (7)
ab37165 has been referenced in 7 publications.
- Xue M et al. Computational identification of mutually exclusive transcriptional drivers dysregulating metastatic microRNAs in prostate cancer. Nat Commun 8:14917 (2017). WB ; Human . PubMed: 28397780
- He H et al. PEG3 Interacts with KAP1 through KRAB-A. PLoS One 11:e0167541 (2016). WB ; Mouse . PubMed: 27898713
- Storm MP et al. Zscan4 is regulated by PI3-kinase and DNA-damaging agents and directly interacts with the transcriptional repressors LSD1 and CtBP2 in mouse embryonic stem cells. PLoS One 9:e89821 (2014). IP, WB ; Mouse . PubMed: 24594919
- Jamaladdin S et al. Histone deacetylase (HDAC) 1 and 2 are essential for accurate cell division and the pluripotency of embryonic stem cells. Proc Natl Acad Sci U S A 111:9840-5 (2014). Mouse . PubMed: 24958871
- Lezina L et al. miR-16 and miR-26a target checkpoint kinases Wee1 and Chk1 in response to p53 activation by genotoxic stress. Cell Death Dis 4:e953 (2013). ChIP . PubMed: 24336073
- Dovey OM et al. Histone deacetylase 1 (HDAC1), but not HDAC2, controls embryonic stem cell differentiation. Proc Natl Acad Sci U S A 107:8242-7 (2010). WB, IP ; Mouse . PubMed: 20404188
- Foster CT et al. Lysine-specific demethylase 1 regulates the embryonic transcriptome and CoREST stability. Mol Cell Biol 30:4851-63 (2010). WB ; Mouse . PubMed: 20713442
