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  1. Link

    kdm1lsd1-antibody-nuclear-marker-ab37165.pdf

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Epigenetics and Nuclear Signaling Chromatin Modifying Enzymes Methylation Lysine demethylation
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Anti-KDM1/LSD1 antibody - Nuclear Marker (ab37165)

  • Datasheet
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Western blot - Anti-KDM1/LSD1 antibody - Nuclear Marker (ab37165)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-KDM1/LSD1 antibody - Nuclear Marker (ab37165)
  • Western blot - Anti-KDM1/LSD1 antibody - Nuclear Marker (ab37165)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-KDM1/LSD1 antibody - Nuclear Marker (ab37165)
  • Western blot - Anti-KDM1/LSD1 antibody - Nuclear Marker (ab37165)

Key features and details

  • Rabbit polyclonal to KDM1/LSD1 - Nuclear Marker
  • Suitable for: IHC-P, WB
  • Reacts with: Mouse, Rat, Human
  • Isotype: IgG

Get better batch-to-batch reproducibility with a recombinant antibody

Product image
Anti-KDM1/LSD1 antibody [EPR6825] - Nuclear Marker and ChIP Grade (ab129195)
  • Research with confidence – consistent and reproducible results with every batch
  • Long-term and scalable supply – powered by recombinant technology for fast production
  • Success from the first experiment – confirmed specificity through extensive validation
  • Ethical standards compliant – production is animal-free

Overview

  • Product name

    Anti-KDM1/LSD1 antibody - Nuclear Marker
    See all KDM1/LSD1 primary antibodies
  • Description

    Rabbit polyclonal to KDM1/LSD1 - Nuclear Marker
  • Host species

    Rabbit
  • Tested applications

    Suitable for: IHC-P, WBmore details
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide within Human KDM1/LSD1 (N terminal). The exact sequence is proprietary. ab37165 was raised against a peptide corresponding to 17 amino acids near the N terminus of human LSD1.

  • General notes

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.2
    Preservative: 0.02% Sodium azide
    Constituent: PBS
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

    • Epigenetics and Nuclear Signaling
    • Chromatin Modifying Enzymes
    • Methylation
    • Lysine demethylation
    • Neuroscience
    • Processes

Associated products

  • Assay kits

    • Histone Demethylase KDM1/LSD1 Inhibitor Assay Kit (ab113456)
    • KDM1/LSD1 Activity Quantification Assay Kit (Colorimetric) (ab113459)
  • Compatible Secondaries

    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
    • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
  • Isotype control

    • Rabbit IgG, polyclonal - Isotype Control (ChIP Grade) (ab171870)
  • Positive Controls

    • P815 whole cell lysate (ab7185)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab37165 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P
Use at an assay dependent concentration.
WB
Use a concentration of 1 - 2 µg/ml. Detects a band of approximately 105 kDa (predicted molecular weight: 93 kDa).
Notes
IHC-P
Use at an assay dependent concentration.
WB
Use a concentration of 1 - 2 µg/ml. Detects a band of approximately 105 kDa (predicted molecular weight: 93 kDa).

Target

  • Function

    Histone demethylase that demethylates both 'Lys-4' (H3K4me) and 'Lys-9' (H3K9me) of histone H3, thereby acting as a coactivator or a corepressor, depending on the context. Acts by oxidizing the substrate by FAD to generate the corresponding imine that is subsequently hydrolyzed. Acts as a corepressor by mediating demethylation of H3K4me, a specific tag for epigenetic transcriptional activation. Demethylates both mono- (H3K4me1) and di-methylated (H3K4me2) H3K4me. May play a role in the repression of neuronal genes. Alone, it is unable to demethylate H3K4me on nucleosomes and requires the presence of RCOR1/CoREST to achieve such activity. Also acts as a coactivator of androgen receptor (ANDR)-dependent transcription, by being recruited to ANDR target genes and mediating demethylation of H3K9me, a specific tag for epigenetic transcriptional repression. The presence of PRKCB in ANDR-containing complexes, which mediates phosphorylation of 'Thr-6' of histone H3 (H3T6ph), a specific tag that prevents demethylation H3K4me, prevents H3K4me demethylase activity of KDM1A. Demethylates di-methylated 'Lys-370' of p53/TP53 which prevents interaction of p53/TP53 with TP53BP1 and represses p53/TP53-mediated transcriptional activation. Demethylates and stabilizes the DNA methylase DNMT1. Required for gastrulation during embryogenesis.
  • Tissue specificity

    Ubiquitously expressed.
  • Sequence similarities

    Belongs to the flavin monoamine oxidase family.
    Contains 1 SWIRM domain.
  • Domain

    The SWIRM domain may act as an anchor site for a histone tail.
  • Cellular localization

    Nucleus.
  • Target information above from: UniProt accession O60341 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 23028 Human
    • Entrez Gene: 99982 Mouse
    • Entrez Gene: 500569 Rat
    • Omim: 609132 Human
    • SwissProt: O60341 Human
    • SwissProt: Q6ZQ88 Mouse
    • Unigene: 591518 Human
    • Unigene: 28540 Mouse
    • Alternative names

      • Amine oxidase (flavin containing) domain 2 antibody
      • Amine oxidase, flavin containing, 2 antibody
      • AOF2 antibody
      • BHC110 antibody
      • BRAF35 HDAC complex protein BHC110 antibody
      • BRAF35-HDAC complex protein BHC110 antibody
      • BRAF35/HDAC complex, 110-kD subunit antibody
      • CPRF antibody
      • EC1 antibody
      • FAD binding protein BRAF35 HDAC complex, 110 kDa subunit antibody
      • Flavin-containing amine oxidase domain-containing protein 2 antibody
      • KDM 1 antibody
      • KDM1 antibody
      • Kdm1a antibody
      • KDM1A_HUMAN antibody
      • KIAA0601 antibody
      • LSD 1 antibody
      • LSD1 antibody
      • Lysine (K) specific demethylase 1 antibody
      • Lysine (K) specific demethylase 1A antibody
      • Lysine demethylase 1A antibody
      • Lysine specific histone demethylase 1 antibody
      • Lysine specific histone demethylase 1A antibody
      • Lysine-specific demethylase 1 antibody
      • Lysine-specific demethylase 1A antibody
      • Lysine-specific histone demethylase 1A antibody
      see all

    Images

    • Western blot - Anti-KDM1/LSD1 antibody - Nuclear Marker (ab37165)
      Western blot - Anti-KDM1/LSD1 antibody - Nuclear Marker (ab37165)
      All lanes : Anti-KDM1/LSD1 antibody - Nuclear Marker (ab37165) at 0.2 µg/ml

      Lane 1 : HEK-293 (human epithelial cell line from embryonic kidney) cell lysate
      Lane 2 : A431 (human epidermoid carcinoma cell line) cell lysate
      Lane 3 : A549 (human lung carcinoma cell line) cell lysate
      Lane 4 : Caco-2 (human colorectal adenocarcinoma cell line) cell lysate
      Lane 5 : Daudi (human Burkitt's lymphoma cell line) cell lysate
      Lane 6 : HeLa (human epithelial cell line from cervix adenocarcinoma) cell lysate
      Lane 7 : HepG2 (human liver hepatocellular carcinoma cell line) cell lysate
      Lane 8 : K562 (human chronic myelogenous leukemia cell line from bone marrow) cell lysate
      Lane 9 : MCF7 (human breast adenocarcinoma cell line) cell lysate
      Lane 10 : Jurkat (human T cell leukemia cell line from peripheral blood) cell lysate
      Lane 11 : SK-N-SH (human neuroblastoma cell line) cell lysate
      Lane 12 : THP-1 (human monocytic leukemia cell line) cell lysate
      Lane 13 : NIH/3T3 (mouse embryo fibroblast cell line) cell lysate
      Lane 14 : YB2/0 (rat spleen lymphoblast cell line) cell lysate

      Lysates/proteins at 15 µg per lane.

      Secondary
      All lanes : Goat anti-rabbit IgG (HRP) at 1/10000 dilution

      Predicted band size: 93 kDa
      Observed band size: 110 kDa why is the actual band size different from the predicted?



      Diluting buffer and concentration: 1 hour at room temperature in 5% NFDM/TBST.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-KDM1/LSD1 antibody - Nuclear Marker (ab37165)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-KDM1/LSD1 antibody - Nuclear Marker (ab37165)

      IHC image of KDM1/LSD1 staining in human prostate formalin fixed paraffin embedded tissue section, performed on a Leica Bond system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab37165, 1µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

      For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

    • Western blot - Anti-KDM1/LSD1 antibody - Nuclear Marker (ab37165)
      Western blot - Anti-KDM1/LSD1 antibody - Nuclear Marker (ab37165)
      All lanes : Anti-KDM1/LSD1 antibody - Nuclear Marker (ab37165) at 0.2 µg/ml

      Lane 1 : Mouse Testis tissue lysate
      Lane 2 : Mouse Spleen tissue lysate
      Lane 3 : Mouse Kidney tissue lysate
      Lane 4 : Mouse Colon tissue lysate
      Lane 5 : Mouse Stomach tissue lysate
      Lane 6 : Mouse Brain tissue lysate
      Lane 7 : Mouse Skin tissue lysate
      Lane 8 : Mouse Skeletal Muscle tissue lysate
      Lane 9 : Mouse Bladder tissue lysate

      Lysates/proteins at 15 µg per lane.

      Secondary
      All lanes : Goat anti-rabbit IgG (HRP) at 1/10000 dilution

      Predicted band size: 93 kDa
      Observed band size: 110 kDa why is the actual band size different from the predicted?



      Diluting buffer and concentration: 1 hour at room temperature in 5% NFDM/TBST.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-KDM1/LSD1 antibody - Nuclear Marker (ab37165)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-KDM1/LSD1 antibody - Nuclear Marker (ab37165)

      Immunohistochemistry (Formalin-fixed paraffin embedded sections) of human breast carcinoma tissue labeling KDM1/LSD1 with ab37165 at 2μg/ml.

    • Western blot - Anti-KDM1/LSD1 antibody - Nuclear Marker (ab37165)
      Western blot - Anti-KDM1/LSD1 antibody - Nuclear Marker (ab37165)
      Lane 1 : Anti-KDM1/LSD1 antibody - Nuclear Marker (ab37165) at 1 µg/ml
      Lane 2 : Anti-KDM1/LSD1 antibody - Nuclear Marker (ab37165) at 2 µg/ml

      All lanes : Mouse P815 cell lysate

      Lysates/proteins at 15 µg per lane.

      Predicted band size: 93 kDa
      Observed band size: 105 kDa why is the actual band size different from the predicted?

    Protocols

    • Western blot protocols
    • Immunohistochemistry protocols

    Click here to view the general protocols

    Datasheets and documents

    • Datasheet download

      Download

    References (10)

    Publishing research using ab37165? Please let us know so that we can cite the reference in this datasheet.

    ab37165 has been referenced in 10 publications.

    • Ding X  et al. MiR-137-3p Inhibits Colorectal Cancer Cell Migration by Regulating a KDM1A-Dependent Epithelial-Mesenchymal Transition. Dig Dis Sci N/A:N/A (2020). PubMed: 32749639
    • Zhou J  et al. HDAC1-mediated deacetylation of LSD1 regulates vascular calcification by promoting autophagy in chronic renal failure. J Cell Mol Med 24:8636-8649 (2020). PubMed: 32596952
    • Harancher MR  et al. Antiviral Properties of the LSD1 Inhibitor SP-2509. J Virol 94:N/A (2020). PubMed: 32699090
    • Xue M  et al. Computational identification of mutually exclusive transcriptional drivers dysregulating metastatic microRNAs in prostate cancer. Nat Commun 8:14917 (2017). WB ; Human . PubMed: 28397780
    • He H  et al. PEG3 Interacts with KAP1 through KRAB-A. PLoS One 11:e0167541 (2016). WB ; Mouse . PubMed: 27898713
    • Storm MP  et al. Zscan4 is regulated by PI3-kinase and DNA-damaging agents and directly interacts with the transcriptional repressors LSD1 and CtBP2 in mouse embryonic stem cells. PLoS One 9:e89821 (2014). IP, WB ; Mouse . PubMed: 24594919
    • Jamaladdin S  et al. Histone deacetylase (HDAC) 1 and 2 are essential for accurate cell division and the pluripotency of embryonic stem cells. Proc Natl Acad Sci U S A 111:9840-5 (2014). Mouse . PubMed: 24958871
    • Lezina L  et al. miR-16 and miR-26a target checkpoint kinases Wee1 and Chk1 in response to p53 activation by genotoxic stress. Cell Death Dis 4:e953 (2013). ChIP . PubMed: 24336073
    • Dovey OM  et al. Histone deacetylase 1 (HDAC1), but not HDAC2, controls embryonic stem cell differentiation. Proc Natl Acad Sci U S A 107:8242-7 (2010). WB, IP ; Mouse . PubMed: 20404188
    • Foster CT  et al. Lysine-specific demethylase 1 regulates the embryonic transcriptome and CoREST stability. Mol Cell Biol 30:4851-63 (2010). WB ; Mouse . PubMed: 20713442

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