Key features and details
- Rabbit polyclonal to KDM2A
- Suitable for: IHC-P
- Reacts with: Human
- Isotype: IgG
Product nameAnti-KDM2A antibody
See all KDM2A primary antibodies
DescriptionRabbit polyclonal to KDM2A
Tested applicationsSuitable for: IHC-Pmore details
Species reactivityReacts with: Human
Predicted to work with: Rabbit, Horse, Guinea pig, Cow, Dog, Pig, Chimpanzee, Gorilla, Orangutan, Bat
- Human Anaplastic Thyroid Carcinoma, Breast Carcinoma, Lung Adenocarcinoma, Ovarian Carcinoma, Skin Basal Cell Carcinoma and Stomach Adenocarcinoma
This product was previously labelled as FBXL11
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In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
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Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage bufferpH: 6.8
Preservative: 0.09% Sodium azide
Constituents: 0.1% BSA, Tris buffered saline
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab99242 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||1/100 - 1/500. Epitope exposure is recommended. Epitope exposure with citrate buffer will enhance staining.|
FunctionHistone demethylase that specifically demethylates 'Lys-36' of histone H3, thereby playing a central role in histone code. Preferentially demethylates dimethylated H3 'Lys-36' residue while it has weak or no activity for mono- and tri-methylated H3 'Lys-36'. May also recognize and bind to some phosphorylated proteins and promote their ubiquitination and degradation. Required to maintain the heterochromatic state. Associates with centromeres and represses transcription of small non-coding RNAs that are encoded by the clusters of satellite repeats at the centromere. Required to sustain centromeric integrity and genomic stability, particularly during mitosis.
Tissue specificityWidely expressed, with highest levels in brain, testis and ovary, followed by lung.
Sequence similaritiesBelongs to the JHDM1 histone demethylase family.
Contains 1 CXXC-type zinc finger.
Contains 1 F-box domain.
Contains 1 JmjC domain.
Contains 6 LRR (leucine-rich) repeats.
Contains 1 PHD-type zinc finger.
DomainThe JmjC domain mediates demethylation activity and is required for satellite silencing.
The CXXC zinc finger preferentially recognizes nonmethylated CpG DNA, and binding is blocked when the CpG DNA is methylated.
modificationsPhosphorylated upon DNA damage, probably by ATM or ATR.
Cellular localizationNucleus > nucleoplasm. Punctate expression throughout the nucleoplasm and enriched in the perinucleolar region. Specifically nucleates at CpG islands where it's presence results in chromatin depleted in H3K36me2.
- Information by UniProt
- [Histone-H3]-lysine-36 demethylase 1A antibody
- CXXC-type zinc finger protein 8 antibody
- CXXC8 antibody
ab99242, at 1/250 dilution, staining KDM2A in formalin-fixed paraffin-embedded Human skin basal cell carcinoma by Immunohistochemistry using DAB staining.
ab99242, at 1/250 dilution, staining KDM2A in formalin-fixed paraffin-embedded Human breast carcinoma by Immunohistochemistry using DAB staining.
ab99242 has been referenced in 2 publications.
- Okamoto K et al. KDM2A-dependent reduction of rRNA transcription on glucose starvation requires HP1 in cells, including triple-negative breast cancer cells. Oncotarget 10:4743-4760 (2019). PubMed: 31413816
- Okamoto K et al. SF-KDM2A binds to ribosomal RNA gene promoter, reduces H4K20me3 level, and elevates ribosomal RNA transcription in breast cancer cells. Int J Oncol N/A:N/A (2017). PubMed: 28350064