Recombinant
RabMAb

Recombinant Anti-KDM2A antibody [EPR18602] (Alexa Fluor® 488) (ab225366)

Overview

  • Product name

    Anti-KDM2A antibody [EPR18602] (Alexa Fluor® 488)
    See all KDM2A primary antibodies
  • Description

    Rabbit monoclonal [EPR18602] to KDM2A (Alexa Fluor® 488)
  • Host species

    Rabbit
  • Conjugation

    Alexa Fluor® 488. Ex: 495nm, Em: 519nm
  • Tested applications

    Suitable for: ICC/IFmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse, Rat
  • Immunogen

    Synthetic peptide within Human KDM2A aa 1-100. The exact sequence is proprietary.
    Database link: Q9Y2K7

  • Positive control

    • ICC/IF: HeLa cells
  • General notes

     This product was previously labelled as FBXL11

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company. The Alexa Fluor® dye included in this product is provided under an intellectual property license from Life Technologies Corporation. As this product contains the Alexa Fluor® dye, the purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). As this product contains the Alexa Fluor® dye the sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: in manufacturing; (ii) to provide a service, information, or data in return for payment (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are sold for use in research. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab225366 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF 1/1000.

This product gave a positive signal in HeLa cells fixed with 4% formaldehyde (10 min)

Target

  • Function

    Histone demethylase that specifically demethylates 'Lys-36' of histone H3, thereby playing a central role in histone code. Preferentially demethylates dimethylated H3 'Lys-36' residue while it has weak or no activity for mono- and tri-methylated H3 'Lys-36'. May also recognize and bind to some phosphorylated proteins and promote their ubiquitination and degradation. Required to maintain the heterochromatic state. Associates with centromeres and represses transcription of small non-coding RNAs that are encoded by the clusters of satellite repeats at the centromere. Required to sustain centromeric integrity and genomic stability, particularly during mitosis.
  • Tissue specificity

    Widely expressed, with highest levels in brain, testis and ovary, followed by lung.
  • Sequence similarities

    Belongs to the JHDM1 histone demethylase family.
    Contains 1 CXXC-type zinc finger.
    Contains 1 F-box domain.
    Contains 1 JmjC domain.
    Contains 6 LRR (leucine-rich) repeats.
    Contains 1 PHD-type zinc finger.
  • Domain

    The JmjC domain mediates demethylation activity and is required for satellite silencing.
    The CXXC zinc finger preferentially recognizes nonmethylated CpG DNA, and binding is blocked when the CpG DNA is methylated.
  • Post-translational
    modifications

    Phosphorylated upon DNA damage, probably by ATM or ATR.
  • Cellular localization

    Nucleus > nucleoplasm. Punctate expression throughout the nucleoplasm and enriched in the perinucleolar region. Specifically nucleates at CpG islands where it's presence results in chromatin depleted in H3K36me2.
  • Information by UniProt
  • Database links

  • Alternative names

    • [Histone-H3]-lysine-36 demethylase 1A antibody
    • CXXC-type zinc finger protein 8 antibody
    • CXXC8 antibody
    • F box / LRR repeat protein 11 antibody
    • F box and leucine rich repeat protein 11 antibody
    • F box protein FBL7 antibody
    • F-box and leucine-rich repeat protein 11 antibody
    • F-box protein FBL7 antibody
    • F-box protein Lilina antibody
    • F-box/LRR-repeat protein 11 antibody
    • FBL11 antibody
    • FBL7 antibody
    • FBXL11 antibody
    • JHDM1A antibody
    • JmjC domain-containing histone demethylation protein 1A antibody
    • Jumonji C domain containing histone demethylase 1A antibody
    • kdm2a antibody
    • KDM2A_HUMAN antibody
    • LILINA antibody
    • Lysine (K) specific demethylase 2A antibody
    • Lysine-specific demethylase 2A antibody
    see all

Images

  • ab225366 staining KDM2A in HeLa cells. The cells were fixed with 4% formaldehyde (10 min), permeabilized with 0.1% Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated overnight at +4°C with ab225366 at 1/1000 dilution (shown in green) and ab195889, Mouse monoclonal to alpha Tubulin (Alexa Fluor® 594), at 1/250 dilution (shown in red). Nuclear DNA was labelled with DAPI (shown in blue).

    Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

References

ab225366 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab225366.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

Sign up