Anti-KDM2B antibody [6F6G11] (ab234082)

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Overview

  • Product name

    Anti-KDM2B antibody [6F6G11]
    See all KDM2B primary antibodies

  • Description

    Mouse monoclonal [6F6G11] to KDM2B

  • Host species

    Mouse

  • Tested applications

    Suitable for: WB, Flow Cytmore details

  • Species reactivity

    Reacts with: Human

  • Immunogen

    Recombinant fragment corresponding to Human KDM2B aa 457-555. Expressed in E.coli.
    Database link: Q8NHM5

  • Positive control

    • WB: Human KDM2B (aa 457-555) recombinant protein; KDM2B (aa 457-555)-hIgGFc-transfected HEK-293 cell lysate; A549 cell lysate. Flow cyt: HeLa cells.

Properties

Applications

Our Abpromise guarantee covers the use of ab234082 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/100 - 1/500. Predicted molecular weight: 153 kDa.
Flow Cyt 1/200 - 1/400.

Target

  • Function

    Histone demethylase that demethylates 'Lys-4' and 'Lys-36' of histone H3, thereby playing a central role in histone code. Preferentially demethylates trimethylated H3 'Lys-4' and dimethylated H3 'Lys-36' residue while it has weak or no activity for mono- and tri-methylated H3 'Lys-36'. Preferentially binds the transcribed region of ribosomal RNA and represses the transcription of ribosomal RNA genes which inhibits cell growth and proliferation. May also serve as a substrate-recognition component of the SCF (SKP1-CUL1-F-box protein)-type E3 ubiquitin ligase complex.

  • Sequence similarities

    Belongs to the JHDM1 histone demethylase family.
    Contains 1 CXXC-type zinc finger.
    Contains 1 F-box domain.
    Contains 1 JmjC domain.
    Contains 7 LRR (leucine-rich) repeats.
    Contains 1 PHD-type zinc finger.

  • Domain

    The JmjC domain mediates demethylation activity (By similarity). It is also required for repression of ribosomal RNA genes.

  • Cellular localization

    Nucleus > nucleolus.
  • Information by UniProt

  • Database links

    • Alternative names

      • [Histone-H3]-lysine-36 demethylase 1B antibody
      • CXXC-type zinc finger protein 2 antibody
      • CXXC2 antibody
      • F box and leucine rich repeat protein 10 antibody
      • F box protein FBL10 antibody
      • F-box and leucine-rich repeat protein 10 antibody
      • F-box protein FBL10 antibody
      • F-box/LRR-repeat protein 10 antibody
      • Fbl10 antibody
      • FBXL10 antibody
      • JEMMA (Jumonji domain EMSY interactor methyltransferase motif) protein antibody
      • JHDM1B antibody
      • JmjC domain-containing histone demethylation protein 1B antibody
      • Jumonji C domain containing histone demethylase 1B antibody
      • Jumonji domain-containing EMSY-interactor methyltransferase motif protein antibody
      • kdm2b antibody
      • KDM2B_HUMAN antibody
      • Lysine (K) specific demethylase 2B antibody
      • Lysine-specific demethylase 2B antibody
      • PCCX2 antibody
      • Protein containing CXXC domain 2 antibody
      • Protein JEMMA antibody
      • Protein-containing CXXC domain 2 antibody
      see all

    Images

    • Western blot - Anti-KDM2B antibody [6F6G11] (ab234082)
      Western blot - Anti-KDM2B antibody [6F6G11] (ab234082)
      All lanes : Anti-KDM2B antibody [6F6G11] (ab234082) at 1/100 dilution

      Lane 1 : HEK-293 (human epithelial cell line from embryonic kidney) cell lysate.
      Lane 2 : KDM2B (aa 457-555)-hIgGFc-transfected HEK-293 cell lysate.

      Predicted band size: 153 kDa

    • Western blot - Anti-KDM2B antibody [6F6G11] (ab234082)
      Western blot - Anti-KDM2B antibody [6F6G11] (ab234082)
      Anti-KDM2B antibody [6F6G11] (ab234082) at 1/100 dilution + Human KDM2B (aa 457-555) recombinant protein.

      Predicted band size: 153 kDa



      Expected recombinant MW: 36.8 kDa

    • Flow Cytometry - Anti-KDM2B antibody [6F6G11] (ab234082)
      Flow Cytometry - Anti-KDM2B antibody [6F6G11] (ab234082)

      Flow cytometric analysis of HeLa (human epithelial cell line from cervix adenocarcinoma) cells labelling KDM2B using ab234082 at 1/200 dilution (green) compared to a negative control (red).

    • Western blot - Anti-KDM2B antibody [6F6G11] (ab234082)
      Western blot - Anti-KDM2B antibody [6F6G11] (ab234082)
      Anti-KDM2B antibody [6F6G11] (ab234082) at 1/100 dilution + A549 (human lung carcinoma cell line) cell lysate.

      Predicted band size: 153 kDa

    References

    ab234082 has not yet been referenced specifically in any publications.

    Publishing research using ab234082? Please let us know so that we can cite the reference in this datasheet.

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