Anti-KDM2B antibody [6F6G11] (ab234082)
![Western blot - Anti-KDM2B antibody [6F6G11] (ab234082)](https://www.abcam.com/ps/products/234/ab234082/Images/ab234082-307728-anti-kdm2b-antibody-6f6g11-western-blot.jpg "Western blot - Anti-KDM2B antibody [6F6G11] (ab234082)")
Key features and details
- Mouse monoclonal [6F6G11] to KDM2B
- Suitable for: WB, Flow Cyt
- Reacts with: Human
- Isotype: IgG2b
Overview
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Product name
Anti-KDM2B antibody [6F6G11]
See all KDM2B primary antibodies -
Description
Mouse monoclonal [6F6G11] to KDM2B -
Host species
Mouse -
Tested applications
Suitable for: WB, Flow Cytmore details -
Species reactivity
Reacts with: Human -
Immunogen
Recombinant fragment corresponding to Human KDM2B aa 457-555. Expressed in E.coli.
Database link: Q8NHM5 -
Positive control
- WB: Human KDM2B (aa 457-555) recombinant protein; KDM2B (aa 457-555)-hIgGFc-transfected HEK-293 cell lysate; A549 cell lysate. Flow cyt: HeLa cells.
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General notes
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.05% Sodium azide
Constituent: PBS -
Concentration information loading... -
Purity
Protein G purified -
Purification notes
Purified from tissue culture supernatant. -
Clonality
Monoclonal -
Clone number
6F6G11 -
Isotype
IgG2b -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
Applications
Our Abpromise guarantee covers the use of ab234082 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | 1/100 - 1/500. Predicted molecular weight: 153 kDa. | |
| Flow Cyt | 1/200 - 1/400. |
Target
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Function
Histone demethylase that demethylates 'Lys-4' and 'Lys-36' of histone H3, thereby playing a central role in histone code. Preferentially demethylates trimethylated H3 'Lys-4' and dimethylated H3 'Lys-36' residue while it has weak or no activity for mono- and tri-methylated H3 'Lys-36'. Preferentially binds the transcribed region of ribosomal RNA and represses the transcription of ribosomal RNA genes which inhibits cell growth and proliferation. May also serve as a substrate-recognition component of the SCF (SKP1-CUL1-F-box protein)-type E3 ubiquitin ligase complex. -
Sequence similarities
Belongs to the JHDM1 histone demethylase family.
Contains 1 CXXC-type zinc finger.
Contains 1 F-box domain.
Contains 1 JmjC domain.
Contains 7 LRR (leucine-rich) repeats.
Contains 1 PHD-type zinc finger. -
Domain
The JmjC domain mediates demethylation activity (By similarity). It is also required for repression of ribosomal RNA genes. -
Cellular localization
Nucleus > nucleolus. - Information by UniProt
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Database links
- Entrez Gene: 84678 Human
- Omim: 609078 Human
- SwissProt: Q8NHM5 Human
- Unigene: 524800 Human
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Alternative names
- [Histone-H3]-lysine-36 demethylase 1B antibody
- CXXC-type zinc finger protein 2 antibody
- CXXC2 antibody
see all
Images
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Western blot - Anti-KDM2B antibody [6F6G11] (ab234082)All lanes : Anti-KDM2B antibody [6F6G11] (ab234082) at 1/100 dilution
Lane 1 : HEK-293 (human epithelial cell line from embryonic kidney) cell lysate.
Lane 2 : KDM2B (aa 457-555)-hIgGFc-transfected HEK-293 cell lysate.
Predicted band size: 153 kDa -
![Western blot - Anti-KDM2B antibody [6F6G11] (ab234082)](https://www.abcam.com/ps/products/234/ab234082/Images/ab234082-307729-anti-kdm2b-antibody-6f6g11-western-blot.jpg)
Western blot - Anti-KDM2B antibody [6F6G11] (ab234082)Anti-KDM2B antibody [6F6G11] (ab234082) at 1/100 dilution + Human KDM2B (aa 457-555) recombinant protein.
Predicted band size: 153 kDaExpected recombinant MW: 36.8 kDa
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![Flow Cytometry - Anti-KDM2B antibody [6F6G11] (ab234082)](https://www.abcam.com/ps/products/234/ab234082/Images/ab234082-307730-anti-kdm2b-antibody-6f6g11-flow-cytometry.jpg)
Flow Cytometry - Anti-KDM2B antibody [6F6G11] (ab234082)Flow cytometric analysis of HeLa (human epithelial cell line from cervix adenocarcinoma) cells labelling KDM2B using ab234082 at 1/200 dilution (green) compared to a negative control (red).
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![Western blot - Anti-KDM2B antibody [6F6G11] (ab234082)](https://www.abcam.com/ps/products/234/ab234082/Images/ab234082-307731-anti-kdm2b-antibody-6f6g11-western-blot.jpg)
Western blot - Anti-KDM2B antibody [6F6G11] (ab234082)Anti-KDM2B antibody [6F6G11] (ab234082) at 1/100 dilution + A549 (human lung carcinoma cell line) cell lysate.
Predicted band size: 153 kDa
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
References (0)
ab234082 has not yet been referenced specifically in any publications.
