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Epigenetics and Nuclear Signaling Chromatin Modifying Enzymes Methylation
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Anti-KDM2B antibody [6F6G11] (ab234082)

  • Datasheet
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Western blot - Anti-KDM2B antibody [6F6G11] (ab234082)
  • Western blot - Anti-KDM2B antibody [6F6G11] (ab234082)
  • Flow Cytometry - Anti-KDM2B antibody [6F6G11] (ab234082)
  • Western blot - Anti-KDM2B antibody [6F6G11] (ab234082)

Key features and details

  • Mouse monoclonal [6F6G11] to KDM2B
  • Suitable for: WB, Flow Cyt
  • Reacts with: Human
  • Isotype: IgG2b

You may also be interested in

Protein
Product image
Recombinant Human KDM2B protein (ab164635)
Secondary
Product image
Goat Anti-Mouse IgG H&L (HRP) (ab205719)

View more associated products

Overview

  • Product name

    Anti-KDM2B antibody [6F6G11]
    See all KDM2B primary antibodies
  • Description

    Mouse monoclonal [6F6G11] to KDM2B
  • Host species

    Mouse
  • Tested applications

    Suitable for: WB, Flow Cytmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Recombinant fragment corresponding to Human KDM2B aa 457-555. Expressed in E.coli.
    Database link: Q8NHM5

  • Positive control

    • WB: Human KDM2B (aa 457-555) recombinant protein; KDM2B (aa 457-555)-hIgGFc-transfected HEK-293 cell lysate; A549 cell lysate. Flow cyt: HeLa cells.
  • General notes

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    Preservative: 0.05% Sodium azide
    Constituent: PBS
  • Concentration information loading...
  • Purity

    Protein G purified
  • Purification notes

    Purified from tissue culture supernatant.
  • Clonality

    Monoclonal
  • Clone number

    6F6G11
  • Isotype

    IgG2b
  • Research areas

    • Epigenetics and Nuclear Signaling
    • Chromatin Modifying Enzymes
    • Methylation
    • Epigenetics and Nuclear Signaling
    • Chromatin Modifying Enzymes
    • Jumonji containing proteins
    • Epigenetics and Nuclear Signaling
    • Chromatin Modifying Enzymes
    • Methylation
    • Lysine demethylation

Associated products

  • Compatible Secondaries

    • Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) (ab150113)
    • Goat Anti-Mouse IgG H&L (HRP) (ab205719)
  • Isotype control

    • Mouse IgG2b, kappa monoclonal [7E10G10] - Isotype Control (ab170192)
  • Positive Controls

    • A549 whole cell lysate (ab7910)
  • Recombinant Protein

    • Recombinant Human KDM2B protein (ab164635)

Applications

Our Abpromise guarantee covers the use of ab234082 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/100 - 1/500. Predicted molecular weight: 153 kDa.
Flow Cyt 1/200 - 1/400.

Target

  • Function

    Histone demethylase that demethylates 'Lys-4' and 'Lys-36' of histone H3, thereby playing a central role in histone code. Preferentially demethylates trimethylated H3 'Lys-4' and dimethylated H3 'Lys-36' residue while it has weak or no activity for mono- and tri-methylated H3 'Lys-36'. Preferentially binds the transcribed region of ribosomal RNA and represses the transcription of ribosomal RNA genes which inhibits cell growth and proliferation. May also serve as a substrate-recognition component of the SCF (SKP1-CUL1-F-box protein)-type E3 ubiquitin ligase complex.
  • Sequence similarities

    Belongs to the JHDM1 histone demethylase family.
    Contains 1 CXXC-type zinc finger.
    Contains 1 F-box domain.
    Contains 1 JmjC domain.
    Contains 7 LRR (leucine-rich) repeats.
    Contains 1 PHD-type zinc finger.
  • Domain

    The JmjC domain mediates demethylation activity (By similarity). It is also required for repression of ribosomal RNA genes.
  • Cellular localization

    Nucleus > nucleolus.
  • Target information above from: UniProt accession Q8NHM5 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 84678 Human
    • Omim: 609078 Human
    • SwissProt: Q8NHM5 Human
    • Unigene: 524800 Human
    • Alternative names

      • [Histone-H3]-lysine-36 demethylase 1B antibody
      • CXXC-type zinc finger protein 2 antibody
      • CXXC2 antibody
      • F box and leucine rich repeat protein 10 antibody
      • F box protein FBL10 antibody
      • F-box and leucine-rich repeat protein 10 antibody
      • F-box protein FBL10 antibody
      • F-box/LRR-repeat protein 10 antibody
      • Fbl10 antibody
      • FBXL10 antibody
      • JEMMA (Jumonji domain EMSY interactor methyltransferase motif) protein antibody
      • JHDM1B antibody
      • JmjC domain-containing histone demethylation protein 1B antibody
      • Jumonji C domain containing histone demethylase 1B antibody
      • Jumonji domain-containing EMSY-interactor methyltransferase motif protein antibody
      • kdm2b antibody
      • KDM2B_HUMAN antibody
      • Lysine (K) specific demethylase 2B antibody
      • Lysine-specific demethylase 2B antibody
      • PCCX2 antibody
      • Protein containing CXXC domain 2 antibody
      • Protein JEMMA antibody
      • Protein-containing CXXC domain 2 antibody
      see all

    Images

    • Western blot - Anti-KDM2B antibody [6F6G11] (ab234082)
      Western blot - Anti-KDM2B antibody [6F6G11] (ab234082)
      All lanes : Anti-KDM2B antibody [6F6G11] (ab234082) at 1/100 dilution

      Lane 1 : HEK-293 (human epithelial cell line from embryonic kidney) cell lysate.
      Lane 2 : KDM2B (aa 457-555)-hIgGFc-transfected HEK-293 cell lysate.

      Predicted band size: 153 kDa

    • Western blot - Anti-KDM2B antibody [6F6G11] (ab234082)
      Western blot - Anti-KDM2B antibody [6F6G11] (ab234082)
      Anti-KDM2B antibody [6F6G11] (ab234082) at 1/100 dilution + Human KDM2B (aa 457-555) recombinant protein.

      Predicted band size: 153 kDa



      Expected recombinant MW: 36.8 kDa

    • Flow Cytometry - Anti-KDM2B antibody [6F6G11] (ab234082)
      Flow Cytometry - Anti-KDM2B antibody [6F6G11] (ab234082)

      Flow cytometric analysis of HeLa (human epithelial cell line from cervix adenocarcinoma) cells labelling KDM2B using ab234082 at 1/200 dilution (green) compared to a negative control (red).

    • Western blot - Anti-KDM2B antibody [6F6G11] (ab234082)
      Western blot - Anti-KDM2B antibody [6F6G11] (ab234082)
      Anti-KDM2B antibody [6F6G11] (ab234082) at 1/100 dilution + A549 (human lung carcinoma cell line) cell lysate.

      Predicted band size: 153 kDa

    Protocols

    To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

    Click here to view the general protocols

    Datasheets and documents

    • Datasheet
  • References (0)

    Publishing research using ab234082? Please let us know so that we can cite the reference in this datasheet.

    ab234082 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

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    There are currently no Customer reviews or Questions for ab234082.
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