Overview

  • Product name

    Anti-KDM3A / JHDM2A antibody
    See all KDM3A / JHDM2A primary antibodies
  • Description

    Rabbit polyclonal to KDM3A / JHDM2A
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IPmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Recombinant fragment within Human KDM3A/ JHDM2A (internal sequence). The exact sequence is proprietary.
    Database link: Q9Y4C1

  • Positive control

    • WB: HEK-293T and HepG2 whole cell extracts. IP: HEK-293T whole cell extract.

Properties

Applications

Our Abpromise guarantee covers the use of ab229454 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/3000. Predicted molecular weight: 147 kDa.
IP 1/100 - 1/500.

Target

  • Function

    Histone demethylase that specifically demethylates 'Lys-9' of histone H3, thereby playing a central role in histone code. Preferentially demethylates mono- and dimethylated H3 'Lys-9' residue, with a preference for dimethylated residue, while it has weak or no activity on trimethylated H3 'Lys-9'. Demethylation of Lys residue generates formaldehyde and succinate. Involved in hormone-dependent transcriptional activation, by participating in recruitment to androgen-receptor target genes, resulting in H3 'Lys-9' demethylation and transcriptional activation. Involved in spermatogenesis by regulating expression of target genes such as PRM1 and TMP1 which are required for packaging and condensation of sperm chromatin. Involved in obesity resistance through regulation of metabolic genes such as PPARA and UCP1.
  • Sequence similarities

    Belongs to the JHDM2 histone demethylase family.
    Contains 1 JmjC domain.
  • Domain

    The JmjC domain and the C6-type zinc-finger are required for the demethylation activity.
    Leu-Xaa-Xaa-Leu-Leu (LXXLL) motifs are known to mediate the association with nuclear receptors.
  • Cellular localization

    Cytoplasm. Nucleus. Nuclear in round spermatids. When spermatids start to elongate, localizes to the cytoplasm where it forms distinct foci which disappear in mature spermatozoa.
  • Information by UniProt
  • Database links

  • Alternative names

    • DKFZp686A24246 antibody
    • DKFZp686P07111 antibody
    • JHDM2A antibody
    • JHMD2A antibody
    • JmjC domain-containing histone demethylation protein 2A antibody
    • JMJD1 antibody
    • JMJD1A antibody
    • Jumonji C domain containing histone demethylase 2A antibody
    • Jumonji domain containing 1 antibody
    • Jumonji domain containing 1A antibody
    • Jumonji domain containing protein 1A antibody
    • Jumonji domain-containing protein 1A antibody
    • Kdm3a antibody
    • KDM3A_HUMAN antibody
    • KIAA0742 antibody
    • Lysine (K) specific demethylase 3A antibody
    • Lysine-specific demethylase 3A antibody
    • Testis specific protein A antibody
    • TSGA antibody
    see all

Images

  • All lanes : Anti-KDM3A / JHDM2A antibody (ab229454) at 1/2000 dilution

    Lane 1 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell extract
    Lane 2 : HepG2 (human liver hepatocellular carcinoma cell line) whole cell extract

    Lysates/proteins at 30 µg per lane.

    Predicted band size: 147 kDa



    5% SDS-PAGE gel.

  • KDM3A / JHDM2A was immunoprecipitated from HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell extract with 4 μg ab229454. Western blot was performed from the immunoprecipitate using ab229454 at 1/500 dilution.

    Lane 1: HEK-293T whole cell extract 50 μg.

    Lane 2: Control IP with preimmune Rabbit IgG instead of ab229454 in HEK-293T whole cell extract.

    Lane 3: ab229454 IP in HEK-293T whole cell extract.

    5 % SDS-PAGE gel.

References

ab229454 has not yet been referenced specifically in any publications.

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