Recombinant
RabMAb

Recombinant Anti-KDM3A / JHDM2A antibody [EPR18875-106] - BSA and Azide free (ab272695)

Submit a review Submit a question

Overview

  • Product name

    Anti-KDM3A / JHDM2A antibody [EPR18875-106] - BSA and Azide free
    See all KDM3A / JHDM2A primary antibodies

  • Description

    Rabbit monoclonal [EPR18875-106] to KDM3A / JHDM2A - BSA and Azide free

  • Host species

    Rabbit

  • Tested applications

    Suitable for: IHC-P, ICC/IF, Flow Cyt, WB, IPmore details
    Unsuitable for: ChIP

  • Species reactivity

    Reacts with: Mouse, Rat, Human

  • Immunogen

    Synthetic peptide within Human KDM3A/ JHDM2A aa 750-850. The exact sequence is proprietary.
    Database link: Q9Y4C1

  • Positive control

    • WB: HeLa, HeLa, Ramos, NIH/3T3 and C6 whole cell lysates. IHC-P: Human testis tissue; Mouse testis tissue; Rat testis tissue. ICC/IF: HeLa cells. FC: HeLa cells treated with 1mM DFO for 24h. IP: Ramos whole cell lysate.

  • General notes

    ab272695 is the carrier-free version of ab243641. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

    ab272695 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.??  

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Applications

Our Abpromise guarantee covers the use of ab272695 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P Use at an assay dependent concentration.
ICC/IF Use at an assay dependent concentration.
Flow Cyt Use at an assay dependent concentration.
WB Use at an assay dependent concentration. Detects a band of approximately 60, 90, 147 kDa (predicted molecular weight: 147 kDa).
IP Use at an assay dependent concentration.
  • Application notes
    Is unsuitable for ChIP.

    • Target

    • Function

      Histone demethylase that specifically demethylates 'Lys-9' of histone H3, thereby playing a central role in histone code. Preferentially demethylates mono- and dimethylated H3 'Lys-9' residue, with a preference for dimethylated residue, while it has weak or no activity on trimethylated H3 'Lys-9'. Demethylation of Lys residue generates formaldehyde and succinate. Involved in hormone-dependent transcriptional activation, by participating in recruitment to androgen-receptor target genes, resulting in H3 'Lys-9' demethylation and transcriptional activation. Involved in spermatogenesis by regulating expression of target genes such as PRM1 and TMP1 which are required for packaging and condensation of sperm chromatin. Involved in obesity resistance through regulation of metabolic genes such as PPARA and UCP1.

    • Sequence similarities

      Belongs to the JHDM2 histone demethylase family.
      Contains 1 JmjC domain.

    • Domain

      The JmjC domain and the C6-type zinc-finger are required for the demethylation activity.
      Leu-Xaa-Xaa-Leu-Leu (LXXLL) motifs are known to mediate the association with nuclear receptors.

    • Cellular localization

      Cytoplasm. Nucleus. Nuclear in round spermatids. When spermatids start to elongate, localizes to the cytoplasm where it forms distinct foci which disappear in mature spermatozoa.
    • Information by UniProt

    • Database links

      see all

    • Alternative names

      • DKFZp686A24246 antibody
      • DKFZp686P07111 antibody
      • JHDM2A antibody
      • JHMD2A antibody
      • JmjC domain-containing histone demethylation protein 2A antibody
      • JMJD1 antibody
      • JMJD1A antibody
      • Jumonji C domain containing histone demethylase 2A antibody
      • Jumonji domain containing 1 antibody
      • Jumonji domain containing 1A antibody
      • Jumonji domain containing protein 1A antibody
      • Jumonji domain-containing protein 1A antibody
      • Kdm3a antibody
      • KDM3A_HUMAN antibody
      • KIAA0742 antibody
      • Lysine (K) specific demethylase 3A antibody
      • Lysine-specific demethylase 3A antibody
      • Testis specific protein A antibody
      • TSGA antibody
      see all

    Images

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-KDM3A / JHDM2A antibody [EPR18875-106] - BSA and Azide free (ab272695)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-KDM3A / JHDM2A antibody [EPR18875-106] - BSA and Azide free (ab272695)

      Immunohistochemical analysis of paraffin-embedded Rat testis tissue labeling KDM3A / JHDM2A with ab243641 at 1/500 dilution followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Positive staining on rat testis (PMID: 17943087). The section was incubated with ab243641 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.

      Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

      Heat mediated antigen retrieval with Citrate buffer (pH 6.0, epitope retrieval solution 1) for 20 mins.

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab243641).

    • Immunocytochemistry/ Immunofluorescence - Anti-KDM3A / JHDM2A antibody [EPR18875-106] - BSA and Azide free (ab272695)
      Immunocytochemistry/ Immunofluorescence - Anti-KDM3A / JHDM2A antibody [EPR18875-106] - BSA and Azide free (ab272695)

      Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized HeLa cells labelling KDM3A / JHDM2A with ab243641 at 1/100 dilution, followed by ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) antibody at 1/1000 dilution (Green). Confocal image showing nuclear staining in HeLa cell line treatment with DFO (1mM) for 24h. ab195889 Anti-alpha Tubulin antibody (Alexa Fluor® 594) was used to counterstain tubulin at 1/1000 dilution (Red). The Nuclear counterstain was DAPI (Blue).

      Secondary antibody only control: Secondary antibody is ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) at 1/1000 dilution.

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab243641).

    • Flow Cytometry - Anti-KDM3A / JHDM2A antibody [EPR18875-106] - BSA and Azide free (ab272695)
      Flow Cytometry - Anti-KDM3A / JHDM2A antibody [EPR18875-106] - BSA and Azide free (ab272695)

      Flow cytometric analysis of 4% paraformaldehyde fixed, 90% methanol permeabilized HeLa cells treated with 1mM DFO for 24h (Red) / Untreated control (Green)  labelling KDM3A / JHDM2A with ab243641 at 1/500 dilution (0.1ug) (Red) / (Green) compared with a Rabbit monoclonal IgG (ab172730) (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). A Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) at 1/2000 dilution was used as the secondary antibody.

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab243641).

    • Immunoprecipitation - Anti-KDM3A / JHDM2A antibody [EPR18875-106] - BSA and Azide free (ab272695)
      Immunoprecipitation - Anti-KDM3A / JHDM2A antibody [EPR18875-106] - BSA and Azide free (ab272695)

      KDM3A / JHDM2A was immunoprecipitated from 0.35 mg Ramos (human Burkitt's lymphoma B lymphocyte), whole cell lysate with ab243641 at 1/30 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab243641 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP)(ab131366) was used at 1/5000 dilution.

      Lane 1: Ramos (human Burkitt's lymphoma B lymphocyte), whole cell lysate 10 ug

      Lane 2: ab243641 IP in Ramos whole cell lysate

      Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab243641 in Ramos whole cell lysate

      Blocking and dilution buffer and concentration: 5% NFDM/TBST.

      Exposure time: 3 minutes.

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab243641).

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-KDM3A / JHDM2A antibody [EPR18875-106] - BSA and Azide free (ab272695)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-KDM3A / JHDM2A antibody [EPR18875-106] - BSA and Azide free (ab272695)

      Immunohistochemical analysis of paraffin-embedded Mouse testis tissue labeling KDM3A / JHDM2A with ab243641 at 1/500 dilution followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Positive staining on mouse testis (PMID: 17943087). The section was incubated with ab243641 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.

      Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

      Heat mediated antigen retrieval with Citrate buffer (pH 6.0, epitope retrieval solution 1) for 20 mins.

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab243641).

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-KDM3A / JHDM2A antibody [EPR18875-106] - BSA and Azide free (ab272695)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-KDM3A / JHDM2A antibody [EPR18875-106] - BSA and Azide free (ab272695)

      Immunohistochemical analysis of paraffin-embedded Human testis tissue labeling KDM3A / JHDM2A with ab243641 at 1/500 dilution followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Nuclear staining on human testis (PMID: 25071150). The section was incubated with ab243641 for 30 mins at room temperature.T he immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.

      Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

      Heat mediated antigen retrieval with Citrate buffer (pH 6.0, epitope retrieval solution 1) for 20 mins.

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab243641).

    Protocols

    To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

    Click here to view the general protocols

    References

    Publishing research using ab272695? Please let us know so that we can cite the reference in this datasheet.

    ab272695 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

    Show All Reviews Q&A
    Submit a review Submit a question

    There are currently no Customer reviews or Questions for ab272695.
    Please use the links above to contact us or submit feedback about this product.

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
    For licensing inquiries, please contact partnerships@abcam.com