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RecombinantRabMAb

Recombinant Anti-KDM3A / JHDM2A antibody [EPR18875-106] - BSA and Azide free (ab272695)

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-KDM3A / JHDM2A antibody [EPR18875-106] - BSA and Azide free (ab272695)
  • Immunocytochemistry/ Immunofluorescence - Anti-KDM3A / JHDM2A antibody [EPR18875-106] - BSA and Azide free (ab272695)
  • Flow Cytometry (Intracellular) - Anti-KDM3A / JHDM2A antibody [EPR18875-106] - BSA and Azide free (ab272695)
  • Immunoprecipitation - Anti-KDM3A / JHDM2A antibody [EPR18875-106] - BSA and Azide free (ab272695)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-KDM3A / JHDM2A antibody [EPR18875-106] - BSA and Azide free (ab272695)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-KDM3A / JHDM2A antibody [EPR18875-106] - BSA and Azide free (ab272695)
  • Anti-KDM3A / JHDM2A antibody [EPR18875-106] - BSA and Azide free (ab272695)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR18875-106] to KDM3A / JHDM2A - BSA and Azide free
  • Suitable for: Flow Cyt (Intra), IHC-P, ICC/IF, WB, IP
  • Reacts with: Mouse, Rat, Human

Conjugates logo Related conjugates and formulations

Unconjugated

Overview

  • Product name

    Anti-KDM3A / JHDM2A antibody [EPR18875-106] - BSA and Azide free
    See all KDM3A / JHDM2A primary antibodies

  • Description

    Rabbit monoclonal [EPR18875-106] to KDM3A / JHDM2A - BSA and Azide free

  • Host species

    Rabbit

  • Tested applications

    Suitable for: Flow Cyt (Intra), IHC-P, ICC/IF, WB, IPmore details
    Unsuitable for: ChIP

  • Species reactivity

    Reacts with: Mouse, Rat, Human

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: HeLa, HeLa, Ramos, NIH/3T3 and C6 whole cell lysates. IHC-P: Human testis tissue; Mouse testis tissue; Rat testis tissue. ICC/IF: HeLa cells. Flow Cyt (intra): HeLa cells treated with 1mM DFO for 24h. IP: Ramos whole cell lysate.

  • General notes

    ab272695 is the carrier-free version of ab243641.

    Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.

    This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

    Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

    This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab272695 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt (Intra)
Use at an assay dependent concentration.
IHC-P
Use at an assay dependent concentration.
ICC/IF
Use at an assay dependent concentration.
WB
Use at an assay dependent concentration. Detects a band of approximately 60, 90, 147 kDa (predicted molecular weight: 147 kDa).
IP
Use at an assay dependent concentration.
Notes
Flow Cyt (Intra)
Use at an assay dependent concentration.
IHC-P
Use at an assay dependent concentration.
ICC/IF
Use at an assay dependent concentration.
WB
Use at an assay dependent concentration. Detects a band of approximately 60, 90, 147 kDa (predicted molecular weight: 147 kDa).
IP
Use at an assay dependent concentration.
Application notes
Is unsuitable for ChIP.

Target

  • Function

    Histone demethylase that specifically demethylates 'Lys-9' of histone H3, thereby playing a central role in histone code. Preferentially demethylates mono- and dimethylated H3 'Lys-9' residue, with a preference for dimethylated residue, while it has weak or no activity on trimethylated H3 'Lys-9'. Demethylation of Lys residue generates formaldehyde and succinate. Involved in hormone-dependent transcriptional activation, by participating in recruitment to androgen-receptor target genes, resulting in H3 'Lys-9' demethylation and transcriptional activation. Involved in spermatogenesis by regulating expression of target genes such as PRM1 and TMP1 which are required for packaging and condensation of sperm chromatin. Involved in obesity resistance through regulation of metabolic genes such as PPARA and UCP1.

  • Sequence similarities

    Belongs to the JHDM2 histone demethylase family.
    Contains 1 JmjC domain.

  • Domain

    The JmjC domain and the C6-type zinc-finger are required for the demethylation activity.
    Leu-Xaa-Xaa-Leu-Leu (LXXLL) motifs are known to mediate the association with nuclear receptors.

  • Cellular localization

    Cytoplasm. Nucleus. Nuclear in round spermatids. When spermatids start to elongate, localizes to the cytoplasm where it forms distinct foci which disappear in mature spermatozoa.
  • Information by UniProt

  • Database links

    see all

  • Alternative names

    • DKFZp686A24246 antibody
    • DKFZp686P07111 antibody
    • JHDM2A antibody
    • JHMD2A antibody
    • JmjC domain-containing histone demethylation protein 2A antibody
    • JMJD1 antibody
    • JMJD1A antibody
    • Jumonji C domain containing histone demethylase 2A antibody
    • Jumonji domain containing 1 antibody
    • Jumonji domain containing 1A antibody
    • Jumonji domain containing protein 1A antibody
    • Jumonji domain-containing protein 1A antibody
    • Kdm3a antibody
    • KDM3A_HUMAN antibody
    • KIAA0742 antibody
    • Lysine (K) specific demethylase 3A antibody
    • Lysine-specific demethylase 3A antibody
    • Testis specific protein A antibody
    • TSGA antibody
    see all

Images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-KDM3A / JHDM2A antibody [EPR18875-106] - BSA and Azide free (ab272695)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-KDM3A / JHDM2A antibody [EPR18875-106] - BSA and Azide free (ab272695)

    Immunohistochemical analysis of paraffin-embedded Rat testis tissue labeling KDM3A / JHDM2A with ab243641 at 1/500 dilution followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Positive staining on rat testis (PMID: 17943087). The section was incubated with ab243641 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

    Heat mediated antigen retrieval with Citrate buffer (pH 6.0, epitope retrieval solution 1) for 20 mins.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab243641).

  • Immunocytochemistry/ Immunofluorescence - Anti-KDM3A / JHDM2A antibody [EPR18875-106] - BSA and Azide free (ab272695)
    Immunocytochemistry/ Immunofluorescence - Anti-KDM3A / JHDM2A antibody [EPR18875-106] - BSA and Azide free (ab272695)

    Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized HeLa cells labelling KDM3A / JHDM2A with ab243641 at 1/100 dilution, followed by ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) antibody at 1/1000 dilution (Green). Confocal image showing nuclear staining in HeLa cell line treatment with DFO (1mM) for 24h. ab195889 Anti-alpha Tubulin antibody (Alexa Fluor® 594) was used to counterstain tubulin at 1/1000 dilution (Red). The Nuclear counterstain was DAPI (Blue).

    Secondary antibody only control: Secondary antibody is ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) at 1/1000 dilution.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab243641).

  • Flow Cytometry (Intracellular) - Anti-KDM3A / JHDM2A antibody [EPR18875-106] - BSA and Azide free (ab272695)
    Flow Cytometry (Intracellular) - Anti-KDM3A / JHDM2A antibody [EPR18875-106] - BSA and Azide free (ab272695)

    Intracellular flow cytometric analysis of 4% paraformaldehyde fixed, 90% methanol permeabilized HeLa cells treated with 1mM DFO for 24h (Red) / Untreated control (Green) labelling KDM3A / JHDM2A with ab243641 at 1/500 dilution (0.1ug) (Red) / (Green) compared with a Rabbit monoclonal IgG (ab172730) (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). A Goat anti rabbit IgG (Alexa Fluor®488, ab150077) at 1/2000 dilution was used as the secondary antibody. 

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab243641). 

     

     

  • Immunoprecipitation - Anti-KDM3A / JHDM2A antibody [EPR18875-106] - BSA and Azide free (ab272695)
    Immunoprecipitation - Anti-KDM3A / JHDM2A antibody [EPR18875-106] - BSA and Azide free (ab272695)

    KDM3A / JHDM2A was immunoprecipitated from 0.35 mg Ramos (human Burkitt's lymphoma B lymphocyte), whole cell lysate with ab243641 at 1/30 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab243641 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP)(ab131366) was used at 1/5000 dilution.

    Lane 1: Ramos (human Burkitt's lymphoma B lymphocyte), whole cell lysate 10 ug

    Lane 2: ab243641 IP in Ramos whole cell lysate

    Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab243641 in Ramos whole cell lysate

    Blocking and dilution buffer and concentration: 5% NFDM/TBST.

    Exposure time: 3 minutes.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab243641).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-KDM3A / JHDM2A antibody [EPR18875-106] - BSA and Azide free (ab272695)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-KDM3A / JHDM2A antibody [EPR18875-106] - BSA and Azide free (ab272695)

    Immunohistochemical analysis of paraffin-embedded Mouse testis tissue labeling KDM3A / JHDM2A with ab243641 at 1/500 dilution followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Positive staining on mouse testis (PMID: 17943087). The section was incubated with ab243641 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

    Heat mediated antigen retrieval with Citrate buffer (pH 6.0, epitope retrieval solution 1) for 20 mins.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab243641).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-KDM3A / JHDM2A antibody [EPR18875-106] - BSA and Azide free (ab272695)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-KDM3A / JHDM2A antibody [EPR18875-106] - BSA and Azide free (ab272695)

    Immunohistochemical analysis of paraffin-embedded Human testis tissue labeling KDM3A / JHDM2A with ab243641 at 1/500 dilution followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Nuclear staining on human testis (PMID: 25071150). The section was incubated with ab243641 for 30 mins at room temperature.T he immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

    Heat mediated antigen retrieval with Citrate buffer (pH 6.0, epitope retrieval solution 1) for 20 mins.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab243641).

  • Anti-KDM3A / JHDM2A antibody [EPR18875-106] - BSA and Azide free (ab272695)
    Anti-KDM3A / JHDM2A antibody [EPR18875-106] - BSA and Azide free (ab272695)

Protocols

To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

Click here to view the general protocols

References (0)

Publishing research using ab272695? Please let us know so that we can cite the reference in this datasheet.

ab272695 has not yet been referenced specifically in any publications.

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