Product nameAnti-KDM3A / JHDM2A antibody
See all KDM3A / JHDM2A primary antibodies
DescriptionRabbit polyclonal to KDM3A / JHDM2A
Tested applicationsSuitable for: WB, IPmore details
Species reactivityReacts with: Human
Predicted to work with: Cow, Dog, Pig, Chimpanzee, Ferret, Rhesus monkey, Gorilla
Synthetic peptide, corresponding to a region within amino acids 1271-1321 of human KDM3A/ JHDM2A
- HeLa whole cell lysate
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage bufferPreservative: 0.09% Sodium azide
Constituents: 1.815% Tris, 1.764% Sodium citrate, 0.021% PBS
Concentration information loading...
PurityImmunogen affinity purified
Purification notesAffinity purified using an epitope specific to KDM3A / JHDM2A immobilized on solid support.
Our Abpromise guarantee covers the use of ab80598 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
WB: 1/2000 - 1/10000. Predicted molecular weight: 147 kDa.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
FunctionHistone demethylase that specifically demethylates 'Lys-9' of histone H3, thereby playing a central role in histone code. Preferentially demethylates mono- and dimethylated H3 'Lys-9' residue, with a preference for dimethylated residue, while it has weak or no activity on trimethylated H3 'Lys-9'. Demethylation of Lys residue generates formaldehyde and succinate. Involved in hormone-dependent transcriptional activation, by participating in recruitment to androgen-receptor target genes, resulting in H3 'Lys-9' demethylation and transcriptional activation. Involved in spermatogenesis by regulating expression of target genes such as PRM1 and TMP1 which are required for packaging and condensation of sperm chromatin. Involved in obesity resistance through regulation of metabolic genes such as PPARA and UCP1.
Sequence similaritiesBelongs to the JHDM2 histone demethylase family.
Contains 1 JmjC domain.
DomainThe JmjC domain and the C6-type zinc-finger are required for the demethylation activity.
Leu-Xaa-Xaa-Leu-Leu (LXXLL) motifs are known to mediate the association with nuclear receptors.
Cellular localizationCytoplasm. Nucleus. Nuclear in round spermatids. When spermatids start to elongate, localizes to the cytoplasm where it forms distinct foci which disappear in mature spermatozoa.
- Information by UniProt
- DKFZp686A24246 antibody
- DKFZp686P07111 antibody
- JHDM2A antibody
All lanes : Anti-KDM3A / JHDM2A antibody (ab80598) at 0.1 µg/ml
Lane 1 : whole HeLa cell lysate at 50 µg
Lane 2 : whole HeLa cell lysate at 15 µg
Lane 3 : whole HeLa cell lysate at 5 µg
Predicted band size: 147 kDa
Observed band size: 171 kDa why is the actual band size different from the predicted?
Additional bands at: 300 kDa, 85 kDa. We are unsure as to the identity of these extra bands.
Detection of Human KDM3A / JHDM2A by Immunoprecipitation. Samples: Whole cell lysate (1 mg for IP, 20% of IP loaded) from HeLa cells. Antibodies: Lane 1: ab80598 used for IP at 3 µg/mg lysate; Lane 2: a control IgG was used for IP. For subsequent Western blot detection, ab80598 was used at 1 µg/ml.
This product has been referenced in:
- Ho JC et al. Inhibition of the H3K9 methyltransferase G9A attenuates oncogenicity and activates the hypoxia signaling pathway. PLoS One 12:e0188051 (2017). Read more (PubMed: 29145444) »
- Hickok JR et al. Nitric oxide modifies global histone methylation by inhibiting Jumonji C domain-containing demethylases. J Biol Chem 288:16004-15 (2013). WB ; Human . Read more (PubMed: 23546878) »