• Product name

    Anti-KDM3A / JHDM2A antibody
    See all KDM3A / JHDM2A primary antibodies
  • Description

    Rabbit polyclonal to KDM3A / JHDM2A
  • Host species

  • Tested applications

    Suitable for: WB, IPmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Cow, Dog, Pig, Chimpanzee, Ferret, Rhesus monkey, Gorilla
  • Immunogen

    Synthetic peptide, corresponding to a region within amino acids 1271-1321 of human KDM3A/ JHDM2A

  • Positive control

    • HeLa whole cell lysate



Our Abpromise guarantee covers the use of ab80598 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
  • Application notes
    IP: Use at 2-5 µg/mg of lysate.
    WB: 1/2000 - 1/10000. Predicted molecular weight: 147 kDa.

    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • Function

      Histone demethylase that specifically demethylates 'Lys-9' of histone H3, thereby playing a central role in histone code. Preferentially demethylates mono- and dimethylated H3 'Lys-9' residue, with a preference for dimethylated residue, while it has weak or no activity on trimethylated H3 'Lys-9'. Demethylation of Lys residue generates formaldehyde and succinate. Involved in hormone-dependent transcriptional activation, by participating in recruitment to androgen-receptor target genes, resulting in H3 'Lys-9' demethylation and transcriptional activation. Involved in spermatogenesis by regulating expression of target genes such as PRM1 and TMP1 which are required for packaging and condensation of sperm chromatin. Involved in obesity resistance through regulation of metabolic genes such as PPARA and UCP1.
    • Sequence similarities

      Belongs to the JHDM2 histone demethylase family.
      Contains 1 JmjC domain.
    • Domain

      The JmjC domain and the C6-type zinc-finger are required for the demethylation activity.
      Leu-Xaa-Xaa-Leu-Leu (LXXLL) motifs are known to mediate the association with nuclear receptors.
    • Cellular localization

      Cytoplasm. Nucleus. Nuclear in round spermatids. When spermatids start to elongate, localizes to the cytoplasm where it forms distinct foci which disappear in mature spermatozoa.
    • Information by UniProt
    • Database links

    • Alternative names

      • DKFZp686A24246 antibody
      • DKFZp686P07111 antibody
      • JHDM2A antibody
      • JHMD2A antibody
      • JmjC domain-containing histone demethylation protein 2A antibody
      • JMJD1 antibody
      • JMJD1A antibody
      • Jumonji C domain containing histone demethylase 2A antibody
      • Jumonji domain containing 1 antibody
      • Jumonji domain containing 1A antibody
      • Jumonji domain containing protein 1A antibody
      • Jumonji domain-containing protein 1A antibody
      • Kdm3a antibody
      • KDM3A_HUMAN antibody
      • KIAA0742 antibody
      • Lysine (K) specific demethylase 3A antibody
      • Lysine-specific demethylase 3A antibody
      • Testis specific protein A antibody
      • TSGA antibody
      see all


    • All lanes : Anti-KDM3A / JHDM2A antibody (ab80598) at 0.1 µg/ml

      Lane 1 : whole HeLa cell lysate at 50 µg
      Lane 2 : whole HeLa cell lysate at 15 µg
      Lane 3 : whole HeLa cell lysate at 5 µg

      Predicted band size: 147 kDa
      Observed band size: 171 kDa
      why is the actual band size different from the predicted?
      Additional bands at: 300 kDa, 85 kDa. We are unsure as to the identity of these extra bands.

    • Detection of Human KDM3A / JHDM2A by Immunoprecipitation. Samples: Whole cell lysate (1 mg for IP, 20% of IP loaded) from HeLa cells. Antibodies: Lane 1: ab80598 used for IP at 3 µg/mg lysate; Lane 2: a control IgG was used for IP. For subsequent Western blot detection, ab80598 was used at 1 µg/ml.


    This product has been referenced in:

    • Ho JC  et al. Inhibition of the H3K9 methyltransferase G9A attenuates oncogenicity and activates the hypoxia signaling pathway. PLoS One 12:e0188051 (2017). Read more (PubMed: 29145444) »
    • Hickok JR  et al. Nitric oxide modifies global histone methylation by inhibiting Jumonji C domain-containing demethylases. J Biol Chem 288:16004-15 (2013). WB ; Human . Read more (PubMed: 23546878) »
    See all 2 Publications for this product

    Customer reviews and Q&As

    1-2 of 2 Abreviews or Q&A

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Xenopus laevis Tissue sections (tadpole limb)
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: citrate buffer
    Yes - 1% Triton X-100
    tadpole limb
    Blocking step
    Roche blocking reagent as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 2% · Temperature: 22°C

    Gufa Lin

    Verified customer

    Submitted Jan 15 2018

    Western blot
    Rat Cell lysate - whole cell (Rat Trophoblast stem cell and human trophoblast ce)
    Loading amount
    100 µg
    Rat Trophoblast stem cell and human trophoblast ce
    0.5% oxygen for 24 hours
    Gel Running Conditions
    Reduced Denaturing
    Blocking step
    Milk as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C

    Abcam user community

    Verified customer

    Submitted Jan 27 2011

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