Product nameAnti-KDM4A / JHDM3A / JMJD2A antibody [13D6] - ChIP Grade
See all KDM4A / JHDM3A / JMJD2A primary antibodies
DescriptionMouse monoclonal [13D6] to KDM4A / JHDM3A / JMJD2A - ChIP Grade
Tested applicationsSuitable for: WB, ICC/IF, ELISA, IP, ChIP, Flow Cytmore details
Species reactivityReacts with: Mouse, Human
His-tagged recombinant protein hJMJD2A aa369-582
- HeLa nuclear extracts or lysates of 293T cells transfected with KDM4A.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle.
Storage bufferConstituent: PBS
Concentration information loading...
- Epigenetics and Nuclear Signaling
- Nuclear Signaling Pathways
- Nuclear Receptors
ChIP Related Products
Our Abpromise guarantee covers the use of ab105953 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/1000 - 1/2000. Predicted molecular weight: 121 kDa.|
|ELISA||Use at an assay dependent dilution.|
|IP||Use at an assay dependent dilution.|
|ChIP||Use at an assay dependent dilution.|
|Flow Cyt||Use 0.5µg for 106 cells.|
FunctionHistone demethylase that specifically demethylates 'Lys-9' and 'Lys-36' residues of histone H3, thereby playing a central role in histone code. Does not demethylate histone H3 'Lys-4', H3 'Lys-27' nor H4 'Lys-20'. Demethylates trimethylated H3 'Lys-9' and H3 'Lys-36' residue, while it has no activity on mono- and dimethylated residues. Demethylation of Lys residue generates formaldehyde and succinate. Participates in transcriptional repression of ASCL2 and E2F-responsive promoters via the recruitment of histone deacetylases and NCOR1, respectively.
Isoform 2: Crucial for muscle differentiation, promotes transcriptional activation of the Myog gene by directing the removal of repressive chromatin marks at its promoter. Lacks the N-terminal demethylase domain.
Sequence similaritiesBelongs to the JHDM3 histone demethylase family.
Contains 1 C2HC pre-PHD-type zinc finger.
Contains 1 JmjC domain.
Contains 1 JmjN domain.
Contains 2 PHD-type zinc fingers.
Contains 2 Tudor domains.
DomainThe 2 Tudor domains recognize and bind methylated histone H3 'Lys-4' residue (H3K4me). Double Tudor domain has an interdigitated structure and the unusual fold is required for its ability to bind methylated histone tails. Trimethylated H3 'Lys-4' (H3K4me3) is bound in a cage of 3 aromatic residues, 2 of which are from the Tudor domain 2, while the binding specificity is determined by side-chain interactions involving residues from the Tudor domain 1. The Tudor domains are also able to bind trimethylated histone H3 'Lys-9' (H3K9me3), di- and trimethylated H4 'Lys-20' (H4K20me2 and H4K20me3). Has high affinity for H4K20me2, blocking recruitment of proteins such as TP53BP1.
modificationsUbiquitinated by RNF8 and RNF168 following DNA damage, leading to its degradation. Degradation promotes accessibility of H4K20me2 mark for DNA repair protein TP53BP1, which is then recruited.
- Information by UniProt
- JHDM3A antibody
- JmjC domain containing histone demethylation protein 3A antibody
- JmjC domain-containing histone demethylation protein 3A antibody
All lanes : Anti-KDM4A / JHDM3A / JMJD2A antibody [13D6] - ChIP Grade (ab105953) at 1/1000 dilution
All lanes :
Predicted band size: 121 kDa
Whole cell lysates derived from 293T transfected with Flag-KDM4A were prepared using PBS+1% Triton plus proteinase inhibitor cocktail.
Whole cell lysates derived from 293T transfected with Flag-KDM4A were prepared using PBS+1% Triton plus proteinase inhibitor cocktail and used for IP (1 ug). WB was performed using anti-Flag antibody.
ab105935 used at 1/500 dilution
The antibody (1/1000 dilution) was tested for IP of KDM4A in HeLa nuclear extracts under three different conditions: 1) regular (25 mM Tris, pH 7.6, 150 mM KCl, 0.1% NP40, 0.5 mM EDTA, 1 mM DTT; 2) ChIP without SDS (ChIP solution C with 1% Triton and 0.1% deoxycholine); 3) ChIP buffer (as 2 plus 0.1% SDS). 10% input is seen upon longer exposure. SDS presence in this case inhibits IP.
Overlay histogram showing HeLa cells stained with ab105953 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab105953, 0.5µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG (1µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed.
This product has been referenced in:
- Li Y et al. JMJD2A facilitates growth and inhibits apoptosis of cervical cancer cells by downregulating tumor suppressor miR-491-5p. Mol Med Rep 19:2489-2496 (2019). Read more (PubMed: 30720092) »
- Wu L et al. Histone Demethylases KDM4A and KDM4C Regulate Differentiation of Embryonic Stem Cells to Endothelial Cells. Stem Cell Reports 5:10-21 (2015). WB, ChIP ; Mouse . Read more (PubMed: 26120059) »