Anti-KDM4A / JHDM3A / JMJD2A antibody (ab24545)
Key features and details
- Rabbit polyclonal to KDM4A / JHDM3A / JMJD2A
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG
Get better batch-to-batch reproducibility with a recombinant antibody
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Overview
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Product name
Anti-KDM4A / JHDM3A / JMJD2A antibody
See all KDM4A / JHDM3A / JMJD2A primary antibodies -
Description
Rabbit polyclonal to KDM4A / JHDM3A / JMJD2A -
Host species
Rabbit -
Tested applications
Suitable for: WBmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Rat -
Immunogen
Recombinant fragment, corresponding to amino acids 592-720 of Human JMJD2A
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Concentration information loading...
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Purity
Whole antiserum -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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ChIP Related Products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab24545 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB | (1) |
1/2000. Detects a band of approximately 130 kDa (predicted molecular weight: 120 kDa).
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Notes |
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WB
1/2000. Detects a band of approximately 130 kDa (predicted molecular weight: 120 kDa). |
Target
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Function
Histone demethylase that specifically demethylates 'Lys-9' and 'Lys-36' residues of histone H3, thereby playing a central role in histone code. Does not demethylate histone H3 'Lys-4', H3 'Lys-27' nor H4 'Lys-20'. Demethylates trimethylated H3 'Lys-9' and H3 'Lys-36' residue, while it has no activity on mono- and dimethylated residues. Demethylation of Lys residue generates formaldehyde and succinate. Participates in transcriptional repression of ASCL2 and E2F-responsive promoters via the recruitment of histone deacetylases and NCOR1, respectively.
Isoform 2: Crucial for muscle differentiation, promotes transcriptional activation of the Myog gene by directing the removal of repressive chromatin marks at its promoter. Lacks the N-terminal demethylase domain. -
Tissue specificity
Ubiquitous. -
Sequence similarities
Belongs to the JHDM3 histone demethylase family.
Contains 1 C2HC pre-PHD-type zinc finger.
Contains 1 JmjC domain.
Contains 1 JmjN domain.
Contains 2 PHD-type zinc fingers.
Contains 2 Tudor domains. -
Domain
The 2 Tudor domains recognize and bind methylated histone H3 'Lys-4' residue (H3K4me). Double Tudor domain has an interdigitated structure and the unusual fold is required for its ability to bind methylated histone tails. Trimethylated H3 'Lys-4' (H3K4me3) is bound in a cage of 3 aromatic residues, 2 of which are from the Tudor domain 2, while the binding specificity is determined by side-chain interactions involving residues from the Tudor domain 1. The Tudor domains are also able to bind trimethylated histone H3 'Lys-9' (H3K9me3), di- and trimethylated H4 'Lys-20' (H4K20me2 and H4K20me3). Has high affinity for H4K20me2, blocking recruitment of proteins such as TP53BP1. -
Post-translational
modificationsUbiquitinated by RNF8 and RNF168 following DNA damage, leading to its degradation. Degradation promotes accessibility of H4K20me2 mark for DNA repair protein TP53BP1, which is then recruited. -
Cellular localization
Nucleus. - Information by UniProt
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Database links
- Entrez Gene: 9682 Human
- Entrez Gene: 313539 Rat
- Omim: 609764 Human
- SwissProt: O75164 Human
- Unigene: 155983 Human
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Alternative names
- JHDM3A antibody
- JmjC domain containing histone demethylation protein 3A antibody
- JmjC domain-containing histone demethylation protein 3A antibody
see all
Images
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Anti-KDM4A / JHDM3A / JMJD2A antibody (ab24545) at 1/2000 dilution + HeLa nuclear extract
Predicted band size: 120 kDa
Observed band size: 130 kDa why is the actual band size different from the predicted?
Protocols
Datasheets and documents
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Datasheet download
References (13)
ab24545 has been referenced in 13 publications.
- Prajapati RS et al. PRDM1 controls the sequential activation of neural, neural crest and sensory progenitor determinants. Development 146:N/A (2019). PubMed: 31806661
- Dobrynin G et al. KDM4A regulates HIF-1 levels through H3K9me3. Sci Rep 7:11094 (2017). PubMed: 28894274
- Garcia J & Lizcano F KDM4C Activity Modulates Cell Proliferation and Chromosome Segregation in Triple-Negative Breast Cancer. Breast Cancer (Auckl) 10:169-175 (2016). ICC/IF ; Human . PubMed: 27840577
- Rosales W et al. Role of Histone Demethylases in Cardiomyocytes Induced to Hypertrophy. Biomed Res Int 2016:2634976 (2016). WB, ICC/IF ; Rat . PubMed: 27722168
- Cascante A et al. Gene-specific methylation control of H3K9 and H3K36 on neurotrophic BDNF versus astroglial GFAP genes by KDM4A/C regulates neural stem cell differentiation. J Mol Biol 426:3467-77 (2014). PubMed: 24747049