Overview

  • Product name

    Anti-KDM4A / JHDM3A / JMJD2A antibody - C-terminal
    See all KDM4A / JHDM3A / JMJD2A primary antibodies
  • Description

    Rabbit polyclonal to KDM4A / JHDM3A / JMJD2A - C-terminal
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WBmore details
  • Species reactivity

    Reacts with: Rat, Human
    Predicted to work with: Mouse, Cow
  • Immunogen

    Recombinant fragment within Human KDM4A/ JHDM3A/ JMJD2A (C terminal). The exact sequence is proprietary.
    Database link: O75164

  • Positive control

    • WB: Rat brain extract; HeLa whole cell and nuclear extracts.

Properties

Applications

Our Abpromise guarantee covers the use of ab228713 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/3000. Predicted molecular weight: 120 kDa.

Target

  • Function

    Histone demethylase that specifically demethylates 'Lys-9' and 'Lys-36' residues of histone H3, thereby playing a central role in histone code. Does not demethylate histone H3 'Lys-4', H3 'Lys-27' nor H4 'Lys-20'. Demethylates trimethylated H3 'Lys-9' and H3 'Lys-36' residue, while it has no activity on mono- and dimethylated residues. Demethylation of Lys residue generates formaldehyde and succinate. Participates in transcriptional repression of ASCL2 and E2F-responsive promoters via the recruitment of histone deacetylases and NCOR1, respectively.
    Isoform 2: Crucial for muscle differentiation, promotes transcriptional activation of the Myog gene by directing the removal of repressive chromatin marks at its promoter. Lacks the N-terminal demethylase domain.
  • Tissue specificity

    Ubiquitous.
  • Sequence similarities

    Belongs to the JHDM3 histone demethylase family.
    Contains 1 C2HC pre-PHD-type zinc finger.
    Contains 1 JmjC domain.
    Contains 1 JmjN domain.
    Contains 2 PHD-type zinc fingers.
    Contains 2 Tudor domains.
  • Domain

    The 2 Tudor domains recognize and bind methylated histone H3 'Lys-4' residue (H3K4me). Double Tudor domain has an interdigitated structure and the unusual fold is required for its ability to bind methylated histone tails. Trimethylated H3 'Lys-4' (H3K4me3) is bound in a cage of 3 aromatic residues, 2 of which are from the Tudor domain 2, while the binding specificity is determined by side-chain interactions involving residues from the Tudor domain 1. The Tudor domains are also able to bind trimethylated histone H3 'Lys-9' (H3K9me3), di- and trimethylated H4 'Lys-20' (H4K20me2 and H4K20me3). Has high affinity for H4K20me2, blocking recruitment of proteins such as TP53BP1.
  • Post-translational
    modifications

    Ubiquitinated by RNF8 and RNF168 following DNA damage, leading to its degradation. Degradation promotes accessibility of H4K20me2 mark for DNA repair protein TP53BP1, which is then recruited.
  • Cellular localization

    Nucleus.
  • Information by UniProt
  • Database links

  • Alternative names

    • JHDM3A antibody
    • JmjC domain containing histone demethylation protein 3A antibody
    • JmjC domain-containing histone demethylation protein 3A antibody
    • JMJD2 antibody
    • JMJD2A antibody
    • jumonji C domain containing histone demethylase 3A antibody
    • Jumonji domain containing 2 antibody
    • Jumonji domain containing 2A antibody
    • Jumonji domain containing protein 2A antibody
    • Jumonji domain-containing protein 2A antibody
    • KDM4A antibody
    • KDM4A_HUMAN antibody
    • KIAA0677 antibody
    • Lysine (K) specific demethylase 4A antibody
    • Lysine-specific demethylase 4A antibody
    • TDRD14A antibody
    • Tudor domain containing 14A antibody
    see all

Images

  • Anti-KDM4A / JHDM3A / JMJD2A antibody - C-terminal (ab228713) at 1/500 dilution + Rat brain extract at 50 µg

    Predicted band size: 120 kDa



    5% SDS-PAGE gel.

  • All lanes : Anti-KDM4A / JHDM3A / JMJD2A antibody - C-terminal (ab228713) at 1/500 dilution

    Lane 1 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell extract
    Lane 2 : HeLa nuclear extract

    Lysates/proteins at 30 µg per lane.

    Secondary
    All lanes : HRP-conjugated anti-rabbit IgG

    Predicted band size: 120 kDa



    7.5% SDS-PAGE gel.

References

ab228713 has not yet been referenced specifically in any publications.

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