Product nameAnti-KDM4C / GASC1 / JMJD2C antibody
See all KDM4C / GASC1 / JMJD2C primary antibodies
DescriptionRabbit polyclonal to KDM4C / GASC1 / JMJD2C
Tested applicationsSuitable for: WB, IPmore details
Species reactivityReacts with: Human
Predicted to work with: Chimpanzee, Gorilla, Orangutan
Synthetic peptide within Human KDM4C/ GASC1/ JMJD2C aa 475-525. The exact sequence is proprietary. (NP_055876.2).
Database link: Q9H3R0
- WB: HeLa and HEK-293T whole cell lysates. IP: HeLa whole cell lysate.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferPreservative: 0.09% Sodium azide
Constituent: Tris citrate/phosphate
pH 7 to 8
Concentration information loading...
PurityImmunogen affinity purified
Purification notesab226480 was affinity purified using an epitope specific to KDM4C / GASC1 / JMJD2C immobilized on solid support.
Our Abpromise guarantee covers the use of ab226480 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/2000 - 1/10000. Predicted molecular weight: 120 kDa.|
|IP||Use at 2-5 µg/mg of lysate.|
FunctionHistone demethylase that specifically demethylates 'Lys-9' and 'Lys-36' residues of histone H3, thereby playing a central role in histone code. Does not demethylate histone H3 'Lys-4', H3 'Lys-27' nor H4 'Lys-20'. Demethylates trimethylated H3 'Lys-9' and H3 'Lys-36' residue, while it has no activity on mono- and dimethylated residues. Demethylation of Lys residue generates formaldehyde and succinate.
Tissue specificityOverexpressed in several esophageal squamous cell carcinomas (ESCs).
Sequence similaritiesBelongs to the JHDM3 histone demethylase family.
Contains 1 JmjC domain.
Contains 1 JmjN domain.
Contains 2 PHD-type zinc fingers.
Contains 2 Tudor domains.
DomainThe 2 Tudor domains recognize and bind methylated histones. Double Tudor domain has an interdigitated structure and the unusual fold is required for its ability to bind methylated histone tails.
- Information by UniProt
- bA146B14.1 antibody
- GASC 1 protein antibody
- GASC-1 protein antibody
All lanes : Anti-KDM4C / GASC1 / JMJD2C antibody (ab226480) at 0.1 µg/ml
Lane 1 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate at 50 µg
Lane 2 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate at 15 µg
Lane 3 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate at 5 µg
Lane 4 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate at 50 µg
Developed using the ECL technique.
Predicted band size: 120 kDa
Exposure time: 3 minutes
KDM4C / GASC1 / JMJD2C was immunoprecipitated from HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate (1 mg for IP, 20% of IP loaded) with ab226480 at 3 µg/mg lysate. Western blot was performed from the immunoprecipitate using ab226480 at 1 µg/ml.
Lane 1: ab226480 (Lot 1) IP in HeLa whole cell lysate.
Lane 2: ab226480 (Lot 2) IP in HeLa whole cell lysate.
Lane 3: Control IgG IP in HeLa whole cell lysate.
Detection: Chemiluminescence with exposure time of 30 seconds.
ab226480 has not yet been referenced specifically in any publications.