Product nameAnti-KDM5A / Jarid1A / RBBP2 antibody [EPR12742]
See all KDM5A / Jarid1A / RBBP2 primary antibodies
DescriptionRabbit monoclonal [EPR12742] to KDM5A / Jarid1A / RBBP2
Tested applicationsSuitable for: Flow Cyt, WBmore details
Unsuitable for: ICC/IF,IHC-P or IP
Species reactivityReacts with: Mouse, Rat, Human
Synthetic peptide within Human KDM5A/ Jarid1A/ RBBP2 aa 1450-1550 (Cysteine residue). The exact sequence is proprietary.
Database link: P29375
- HeLa, 293T, MCF7 and NCCIT cell lysates; permeabilized NCCIT cells.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol, 0.05% BSA, 50% Tissue culture supernatant
PurityTissue culture supernatant
- Epigenetics and Nuclear Signaling
- Nuclear Signaling Pathways
- Nuclear Receptors
Our Abpromise guarantee covers the use of ab177486 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|Flow Cyt||1/10 - 1/100.
ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
|WB||1/1000 - 1/5000. Detects a band of approximately 200 kDa (predicted molecular weight: 192 kDa).|
FunctionHistone demethylase that specifically demethylates 'Lys-4' of histone H3, thereby playing a central role in histone code. Does not demethylate histone H3 'Lys-9', H3 'Lys-27', H3 'Lys-36', H3 'Lys-79' or H4 'Lys-20'. Demethylates trimethylated and dimethylated but not monomethylated H3 'Lys-4'. May stimulate transcription mediated by nuclear receptors. May be involved in transcriptional regulation of Hox proteins during cell differentiation. May participate in transcriptional repression of cytokines such as CXCL12.
Sequence similaritiesBelongs to the JARID1 histone demethylase family.
Contains 1 ARID domain.
Contains 1 JmjC domain.
Contains 1 JmjN domain.
Contains 3 PHD-type zinc fingers.
DomainThe GSGFP motif is required for the interaction with SUZ12.
Cellular localizationNucleus > nucleolus. Occupies promoters of genes involved in RNA metabolism and mitochondrial function.
- Information by UniProt
- Histone demethylase JARID1A antibody
- JARID1A antibody
- Jumonji/ARID domain containing protein 1A antibody
Lane 1: Wild-type HAP1 whole cell lysate (20 µg)
Lane 2: KDM5A knockout HAP1 whole cell lysate (20 µg)
Lane 3: HeLa whole cell lysate (20 µg)
Lane 4: HEK293 whole cell lysate (20 µg)
Lanes 1 - 4: Merged signal (red and green). Green - ab177486 observed at 240 kDa. Red - loading control, ab18058, observed at 130 kDa.
ab177486 was shown to specifically react with KDM5A in wild-type HAP1 cells. No bands were observed when KDM5A knockout samples were used. Wild-type and KDM5A knockout samples were subjected to SDS-PAGE. Ab177486 and ab18058 (Mouse anti Vinculin loading control) were incubated overnight at 4°C at 1/1000 dilution and 1/10000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed ab216776 secondary antibodies at 1/20000 dilution for 1 hour at room temperature before imaging.
Flow Cytometric analysis of permeabilized NCCIT cells labeling KDM5A / Jarid1A / RBBP2 with ab177486 at 1/10 dilution (red) or a rabbit IgG (negative) (green).
All lanes : Anti-KDM5A / Jarid1A / RBBP2 antibody [EPR12742] (ab177486) at 1/1000 dilution
Lane 1 : HeLa cell lysate
Lane 2 : 293T cell lysate
Lane 3 : MCF7 cell lysate
Lane 4 : NCCIT cell lysate
Lysates/proteins at 10 µg per lane.
All lanes : Goat anti-rabbit HRP at 1/2000 dilution
Predicted band size: 192 kDa
This product has been referenced in:
- Chao WH et al. Dendrobium nobile Lindley and its bibenzyl component moscatilin are able to protect retinal cells from ischemia/hypoxia by dowregulating placental growth factor and upregulating Norrie disease protein. BMC Complement Altern Med 18:193 (2018). Read more (PubMed: 29933759) »