Recombinant Anti-KDM5A / Jarid1A / RBBP2 antibody [EPR12742] - BSA and Azide free (ab249990)
![Western blot - Anti-KDM5A / Jarid1A / RBBP2 antibody [EPR12742] - BSA and Azide free (ab249990)](https://www.abcam.com/ps/products/249/ab249990/Images/ab249990-406440-anti-kdm5a-jarid1a-rbbp2-antibody-epr12742-bsa-and-azide-free-western-blot.jpg "Western blot - Anti-KDM5A / Jarid1A / RBBP2 antibody [EPR12742] - BSA and Azide free (ab249990)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR12742] to KDM5A / Jarid1A / RBBP2 - BSA and Azide free
- Suitable for: Flow Cyt (Intra), WB
- Knockout validated
- Reacts with: Human
Related conjugates and formulations
Overview
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Product name
Anti-KDM5A / Jarid1A / RBBP2 antibody [EPR12742] - BSA and Azide free
See all KDM5A / Jarid1A / RBBP2 primary antibodies -
Description
Rabbit monoclonal [EPR12742] to KDM5A / Jarid1A / RBBP2 - BSA and Azide free -
Host species
Rabbit -
Tested applications
Suitable for: Flow Cyt (Intra), WBmore details
Unsuitable for: ICC/IF,IHC-P or IP -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat
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Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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General notes
ab249990 is the carrier-free version of ab177486.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
Storage buffer
pH: 7.2
Constituent: PBS -
Carrier free
Yes -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR12742 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Compatible Secondaries
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Conjugation kits
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Isotype control
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Positive Controls
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab249990 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| Flow Cyt (Intra) |
Use at an assay dependent concentration.
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| WB |
Use at an assay dependent concentration. Detects a band of approximately 200 kDa (predicted molecular weight: 192 kDa).
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| Notes |
|---|
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Flow Cyt (Intra)
Use at an assay dependent concentration. |
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WB
Use at an assay dependent concentration. Detects a band of approximately 200 kDa (predicted molecular weight: 192 kDa). |
Target
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Function
Histone demethylase that specifically demethylates 'Lys-4' of histone H3, thereby playing a central role in histone code. Does not demethylate histone H3 'Lys-9', H3 'Lys-27', H3 'Lys-36', H3 'Lys-79' or H4 'Lys-20'. Demethylates trimethylated and dimethylated but not monomethylated H3 'Lys-4'. May stimulate transcription mediated by nuclear receptors. May be involved in transcriptional regulation of Hox proteins during cell differentiation. May participate in transcriptional repression of cytokines such as CXCL12. -
Sequence similarities
Belongs to the JARID1 histone demethylase family.
Contains 1 ARID domain.
Contains 1 JmjC domain.
Contains 1 JmjN domain.
Contains 3 PHD-type zinc fingers. -
Domain
The GSGFP motif is required for the interaction with SUZ12. -
Cellular localization
Nucleus > nucleolus. Occupies promoters of genes involved in RNA metabolism and mitochondrial function. - Information by UniProt
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Database links
- Entrez Gene: 5927 Human
- Entrez Gene: 214899 Mouse
- Entrez Gene: 312678 Rat
- Omim: 180202 Human
- SwissProt: P29375 Human
- SwissProt: Q3UXZ9 Mouse
- Unigene: 76272 Human
- Unigene: 404761 Mouse
see all -
Alternative names
- Histone demethylase JARID1A antibody
- JARID1A antibody
- Jumonji/ARID domain containing protein 1A antibody
see all
Images
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Western blot - Anti-KDM5A / Jarid1A / RBBP2 antibody [EPR12742] - BSA and Azide free (ab249990)
This data was developed using ab177486, the same antibody clone in a different buffer formulation.
Lane 1: Wild-type HAP1 whole cell lysate (20 µg)
Lane 2: KDM5A knockout HAP1 whole cell lysate (20 µg)
Lane 3: HeLa whole cell lysate (20 µg)
Lane 4: HEK293 whole cell lysate (20 µg)
Lanes 1 - 4: Merged signal (red and green). Green - ab177486 observed at 240 kDa. Red - loading control, ab18058, observed at 130 kDa.
ab177486 was shown to specifically react with KDM5A in wild-type HAP1 cells. No bands were observed when KDM5A knockout samples were used. Wild-type and KDM5A knockout samples were subjected to SDS-PAGE. ab177486 and ab18058 (Mouse anti-Vinculin loading control) were incubated overnight at 4°C at 1/1000 dilution and 1/10000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed ab216776 secondary antibodies at 1/20000 dilution for 1 hour at room temperature before imaging.
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![Flow Cytometry (Intracellular) - Anti-KDM5A / Jarid1A / RBBP2 antibody [EPR12742] - BSA and Azide free (ab249990)](https://www.abcam.com/ps/products/249/ab249990/Images/ab249990-2-anti-kdm5a-jarid1a-rbbp2-antibody-epr12742-bsa-and-azide-free-flow-cytometry.jpg)
Flow Cytometry (Intracellular) - Anti-KDM5A / Jarid1A / RBBP2 antibody [EPR12742] - BSA and Azide free (ab249990) -
![Western blot - Anti-KDM5A / Jarid1A / RBBP2 antibody [EPR12742] - BSA and Azide free (ab249990)](https://www.abcam.com/ps/products/249/ab249990/Images/ab249990-398865-anti-kdm5a-jarid1a-rbbp2-antibody-epr12742-bsa-and-azide-free-western-blot.jpg)
Western blot - Anti-KDM5A / Jarid1A / RBBP2 antibody [EPR12742] - BSA and Azide free (ab249990)All lanes : Anti-KDM5A / Jarid1A / RBBP2 antibody [EPR12742] (ab177486) at 1/1000 dilution
Lane 1 : HeLa cell lysate
Lane 2 : 293T cell lysate
Lane 3 : MCF7 cell lysate
Lane 4 : NCCIT cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat anti-rabbit HRP at 1/2000 dilution
Predicted band size: 192 kDaThis data was developed using ab177486, the same antibody clone in a different buffer formulation.
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![Anti-KDM5A / Jarid1A / RBBP2 antibody [EPR12742] - BSA and Azide free (ab249990)](https://www.abcam.com/ps/products/249/ab249990/Images/ab249990-4-benefits-of-recombinant-antibodies.png)
Anti-KDM5A / Jarid1A / RBBP2 antibody [EPR12742] - BSA and Azide free (ab249990)
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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Datasheet download
Certificate of Compliance
References (0)
ab249990 has not yet been referenced specifically in any publications.
