Validated using a knockout cell line
Recombinant
RabMAb

Anti-KDM5A / Jarid1A / RBBP2 antibody [EPR12742] (ab177486)

Overview

  • Product name
    Anti-KDM5A / Jarid1A / RBBP2 antibody [EPR12742]
    See all KDM5A / Jarid1A / RBBP2 primary antibodies
  • Description
    Rabbit monoclonal [EPR12742] to KDM5A / Jarid1A / RBBP2
  • Host species
    Rabbit
  • Tested applications
    Suitable for: Flow Cyt, WBmore details
    Unsuitable for: ICC/IF,IHC-P or IP
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide within Human KDM5A/ Jarid1A/ RBBP2 aa 1450-1550 (Cysteine residue). The exact sequence is proprietary.
    Database link: P29375

  • Positive control
    • HeLa, 293T, MCF7 and NCCIT cell lysates; permeabilized NCCIT cells.
  • General notes

     

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab177486 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt 1/10 - 1/100.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

 

WB 1/1000 - 1/5000. Detects a band of approximately 200 kDa (predicted molecular weight: 192 kDa).
  • Application notes
    Is unsuitable for ICC/IF,IHC-P or IP.
  • Target

    • Function
      Histone demethylase that specifically demethylates 'Lys-4' of histone H3, thereby playing a central role in histone code. Does not demethylate histone H3 'Lys-9', H3 'Lys-27', H3 'Lys-36', H3 'Lys-79' or H4 'Lys-20'. Demethylates trimethylated and dimethylated but not monomethylated H3 'Lys-4'. May stimulate transcription mediated by nuclear receptors. May be involved in transcriptional regulation of Hox proteins during cell differentiation. May participate in transcriptional repression of cytokines such as CXCL12.
    • Sequence similarities
      Belongs to the JARID1 histone demethylase family.
      Contains 1 ARID domain.
      Contains 1 JmjC domain.
      Contains 1 JmjN domain.
      Contains 3 PHD-type zinc fingers.
    • Domain
      The GSGFP motif is required for the interaction with SUZ12.
    • Cellular localization
      Nucleus > nucleolus. Occupies promoters of genes involved in RNA metabolism and mitochondrial function.
    • Information by UniProt
    • Database links
    • Alternative names
      • Histone demethylase JARID1A antibody
      • JARID1A antibody
      • Jumonji/ARID domain containing protein 1A antibody
      • Jumonji/ARID domain-containing protein 1A antibody
      • Kdm5a antibody
      • KDM5A_HUMAN antibody
      • Lysine-specific demethylase 5A antibody
      • RBBP-2 antibody
      • RBBP2 antibody
      • RBP2 antibody
      • Retinoblastoma binding protein 2 antibody
      • Retinoblastoma-binding protein 2 antibody
      see all

    Images

    • Lane 1: Wild-type HAP1 whole cell lysate (20 µg)
      Lane 2: KDM5A knockout HAP1 whole cell lysate (20 µg)
      Lane 3: HeLa whole cell lysate (20 µg)
      Lane 4: HEK293 whole cell lysate (20 µg)

      Lanes 1 - 4: Merged signal (red and green). Green - ab177486 observed at 240 kDa. Red - loading control, ab18058, observed at 130 kDa.

      ab177486 was shown to specifically react with KDM5A in wild-type HAP1 cells. No bands were observed when KDM5A knockout samples were used. Wild-type and KDM5A knockout samples were subjected to SDS-PAGE. Ab177486 and ab18058 (Mouse anti Vinculin loading control) were incubated overnight at 4°C at 1/1000 dilution and 1/10000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed ab216776 secondary antibodies at 1/20000 dilution for 1 hour at room temperature before imaging.

       

    • Flow Cytometric analysis of permeabilized NCCIT cells labeling KDM5A / Jarid1A / RBBP2 with ab177486 at 1/10 dilution (red) or a rabbit IgG (negative) (green).

    • All lanes : Anti-KDM5A / Jarid1A / RBBP2 antibody [EPR12742] (ab177486) at 1/1000 dilution

      Lane 1 : HeLa cell lysate
      Lane 2 : 293T cell lysate
      Lane 3 : MCF7 cell lysate
      Lane 4 : NCCIT cell lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : Goat anti-rabbit HRP at 1/2000 dilution

      Predicted band size: 192 kDa

    References

    ab177486 has not yet been referenced specifically in any publications.

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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