Product nameAnti-KDM5B / PLU1 / Jarid1B antibody
See all KDM5B / PLU1 / Jarid1B primary antibodies
DescriptionRabbit polyclonal to KDM5B / PLU1 / Jarid1B
Tested applicationsSuitable for: ICC/IF, Flow Cytmore details
Species reactivityReacts with: Human
- HeLa cells.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.40
Preservative: 0.02% Sodium azide
Constituents: 0.1% BSA, 50% Glycerol, 49% Tris buffered saline
Concentration information loading...
PurityImmunogen affinity purified
- Epigenetics and Nuclear Signaling
- Nuclear Signaling Pathways
- Nuclear Receptors
Our Abpromise guarantee covers the use of ab186056 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ICC/IF||1/80 - 1/100.|
ab171870 - Rabbit polyclonal IgG, is suitable for use as an isotype control with this antibody.
FunctionHistone demethylase that demethylates 'Lys-4' of histone H3, thereby playing a central role in histone code. Does not demethylate histone H3 'Lys-9' or H3 'Lys-27'. Demethylates trimethylated, dimethylated and monomethylated H3 'Lys-4'. Acts as a transcriptional corepressor for FOXG1B and PAX9. Favors the proliferation of breast cancer cells by repressing tumor suppressor genes such as BRCA1 and HOXA5. In contrast, may act as a tumor suppressor for melanoma.
Tissue specificityUbiquitously expressed, with highest levels in testis. Down-regulated in melanoma and glioblastoma. Up-regulated in breast cancer (at protein level).
Sequence similaritiesBelongs to the JARID1 histone demethylase family.
Contains 1 ARID domain.
Contains 1 JmjC domain.
Contains 1 JmjN domain.
Contains 3 PHD-type zinc fingers.
DomainBoth the JmjC domain and the JmjN domain are required for enzymatic activity.
The 2 first PHD-type zinc finger domains are required for transcription repression activity.
- Information by UniProt
- Cancer/testis antigen 31 antibody
- CT31 antibody
- Histone demethylase JARID1B antibody
Immunofluorescence analysis of methanol-fixed HeLa cells, labeling KDM5B / PLU1 / Jarid1B with either A) Control without primary antibody, or B) ab186056 at 10 µg/ml. As secondary antibody goat anti-rabbit IgG FITC was used at a 1/200 dilution.
Flow cytometry analysis of fixed HeLa cells labeling KDM5B / PLU1 / Jarid1B using: 1) A rabbit IgG-FITC antibody at 0.01 µg/ml (red); 2) ab186056 at 5 µg/ml (yellow); 3) ab186056 at 10 µg/ml (blue). Immune complexes were detected with goat anti-rabbit IgG FITC at a 1/200 dilution.
ab186056 has not yet been referenced specifically in any publications.