Overview

  • Product name

    Anti-KDM5B / PLU1 / Jarid1B antibody
    See all KDM5B / PLU1 / Jarid1B primary antibodies
  • Description

    Rabbit polyclonal to KDM5B / PLU1 / Jarid1B
  • Host species

    Rabbit
  • Tested applications

    Suitable for: IHC-P, WBmore details
  • Species reactivity

    Reacts with: Mouse, Human
  • Immunogen

    Synthetic peptide corresponding to Human KDM5B/ PLU1/ Jarid1B (internal sequence). NP_006609.
    Database link: Q9UGL1

  • Positive control

    • HeLa and MCF7 cell lysates; Human brain tissue.

Properties

Applications

Our Abpromise guarantee covers the use of ab198884 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/50 - 1/200.
WB 1/200 - 1/1000. Predicted molecular weight: 176 kDa.

Target

  • Function

    Histone demethylase that demethylates 'Lys-4' of histone H3, thereby playing a central role in histone code. Does not demethylate histone H3 'Lys-9' or H3 'Lys-27'. Demethylates trimethylated, dimethylated and monomethylated H3 'Lys-4'. Acts as a transcriptional corepressor for FOXG1B and PAX9. Favors the proliferation of breast cancer cells by repressing tumor suppressor genes such as BRCA1 and HOXA5. In contrast, may act as a tumor suppressor for melanoma.
  • Tissue specificity

    Ubiquitously expressed, with highest levels in testis. Down-regulated in melanoma and glioblastoma. Up-regulated in breast cancer (at protein level).
  • Sequence similarities

    Belongs to the JARID1 histone demethylase family.
    Contains 1 ARID domain.
    Contains 1 JmjC domain.
    Contains 1 JmjN domain.
    Contains 3 PHD-type zinc fingers.
  • Domain

    Both the JmjC domain and the JmjN domain are required for enzymatic activity.
    The 2 first PHD-type zinc finger domains are required for transcription repression activity.
  • Cellular localization

    Nucleus.
  • Information by UniProt
  • Database links

  • Alternative names

    • Cancer/testis antigen 31 antibody
    • CT31 antibody
    • Histone demethylase JARID1B antibody
    • JARID1B antibody
    • Jumonji AT rich interactive domain 1B antibody
    • Jumonji/ARID domain-containing protein 1B antibody
    • Kdm5b antibody
    • KDM5B_HUMAN antibody
    • Lysine (K) specific demethylase 5B antibody
    • Lysine demethylase 5B antibody
    • Lysine specific demethylase 5B antibody
    • Lysine-specific demethylase 5B antibody
    • PLU-1 antibody
    • PLU1 antibody
    • PPP1R98 antibody
    • Protein phosphatase 1 regulatory subunit 98 antibody
    • PUT1 antibody
    • putative DNA/chromatin binding motif antibody
    • RBBP2H1A antibody
    • RBP2 like antibody
    • RBP2-H1 antibody
    • Retinoblastoma-binding protein 2 homolog 1 antibody
    • Retinoblastoma-binding protein 2 homolog 1A antibody
    see all

Images

  • All lanes : Anti-KDM5B / PLU1 / Jarid1B antibody (ab198884) at 1/200 dilution

    Lane 1 : HeLa cell lysate
    Lane 2 : MCF7 cell lysate

    Lysates/proteins at 40 µg per lane.

    Developed using the ECL technique.

    Predicted band size: 176 kDa


    Exposure time: 10 minutes


    Gel: 6% SDS-PAGE

  • Immunohistochemical analysis of paraffin-embedded Human brain tissue labeling KDM5B / PLU1 / Jarid1B with ab198884 at 1/40 dilution.

References

This product has been referenced in:

  • Fang L  et al. Jumonji AT-rich interactive domain 1B overexpression is associated with the development and progression of glioma. Int J Mol Med 38:172-82 (2016). WB, IHC ; Human . Read more (PubMed: 27246838) »
See 1 Publication for this product

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