Key features and details
- Rabbit polyclonal to KDM5B / PLU1 / Jarid1B
- Suitable for: IHC-P, WB
- Reacts with: Mouse, Human
- Isotype: IgG
Product nameAnti-KDM5B / PLU1 / Jarid1B antibody
See all KDM5B / PLU1 / Jarid1B primary antibodies
DescriptionRabbit polyclonal to KDM5B / PLU1 / Jarid1B
Tested applicationsSuitable for: IHC-P, WBmore details
Species reactivityReacts with: Mouse, Human
Synthetic peptide corresponding to Human KDM5B/ PLU1/ Jarid1B (internal sequence). NP_006609.
Database link: Q9UGL1
- HeLa and MCF7 cell lysates; Human brain tissue.
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Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.4
Preservative: 0.05% Sodium azide
Constituents: 50% Glycerol (glycerin, glycerine), 49% PBS
Concentration information loading...
PurityImmunogen affinity purified
- Epigenetics and Nuclear Signaling
- Nuclear Signaling Pathways
- Nuclear Receptors
Our Abpromise guarantee covers the use of ab198884 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||1/50 - 1/200.|
|WB||1/200 - 1/1000. Predicted molecular weight: 176 kDa.|
FunctionHistone demethylase that demethylates 'Lys-4' of histone H3, thereby playing a central role in histone code. Does not demethylate histone H3 'Lys-9' or H3 'Lys-27'. Demethylates trimethylated, dimethylated and monomethylated H3 'Lys-4'. Acts as a transcriptional corepressor for FOXG1B and PAX9. Favors the proliferation of breast cancer cells by repressing tumor suppressor genes such as BRCA1 and HOXA5. In contrast, may act as a tumor suppressor for melanoma.
Tissue specificityUbiquitously expressed, with highest levels in testis. Down-regulated in melanoma and glioblastoma. Up-regulated in breast cancer (at protein level).
Sequence similaritiesBelongs to the JARID1 histone demethylase family.
Contains 1 ARID domain.
Contains 1 JmjC domain.
Contains 1 JmjN domain.
Contains 3 PHD-type zinc fingers.
DomainBoth the JmjC domain and the JmjN domain are required for enzymatic activity.
The 2 first PHD-type zinc finger domains are required for transcription repression activity.
- Information by UniProt
- Cancer/testis antigen 31 antibody
- CT31 antibody
- Histone demethylase JARID1B antibody
All lanes : Anti-KDM5B / PLU1 / Jarid1B antibody (ab198884) at 1/200 dilution
Lane 1 : HeLa cell lysate
Lane 2 : MCF7 cell lysate
Lysates/proteins at 40 µg per lane.
Developed using the ECL technique.
Predicted band size: 176 kDa
Exposure time: 10 minutes
Gel: 6% SDS-PAGE
Immunohistochemical analysis of paraffin-embedded Human brain tissue labeling KDM5B / PLU1 / Jarid1B with ab198884 at 1/40 dilution.
ab198884 has been referenced in 2 publications.
- Sun H et al. Prognostic value of elevated KDM5B expression in patients with laryngeal squamous cell carcinoma. Int J Clin Exp Pathol 12:3500-3506 (2019). PubMed: 31934196
- Fang L et al. Jumonji AT-rich interactive domain 1B overexpression is associated with the development and progression of glioma. Int J Mol Med 38:172-82 (2016). WB, IHC ; Human . PubMed: 27246838