For the best experience on the Abcam website please upgrade to a modern browser such as Google Chrome

Hello. We're improving abcam.com and we'd welcome your feedback.

Hello. We're improving abcam.com and we'd welcome your feedback.

Infomation icon

We haven't added this to the BETA yet

New BETA website

New BETA website

Hello. We're improving abcam.com and we'd welcome your feedback.

Take a look at our BETA site and see what we’ve done so far.

Switch on our new BETA site

Now available

Search and browse selected products

  • A selection of primary antibodies

Purchase these through your usual distributor

In the coming months

  • Additional product types
  • Supporting content
  • Sign in to your account
  • Purchase online
United States
Your country/region is currently set to:

If incorrect, please enter your country/region into the box below, to view site information related to your country/region.

Call (888) 77-ABCAM (22226) or contact us
Need help? Contact us

  • My account
  • Sign out
Sign in or Register with us

Welcome

Sign in or

Don't have an account?

Register with us
My basket
Quick order
Abcam homepage

  • Research Products
    By product type
    Primary antibodies
    Secondary antibodies
    ELISA and Matched Antibody Pair Kits
    Cell and tissue imaging tools
    Cellular and biochemical assays
    Proteins and Peptides
    By product type
    Proteomics tools
    Agonists, activators, antagonists and inhibitors
    Cell lines and Lysates
    Multiplex miRNA assays
    Multiplex Assays
    By research area
    Cancer
    Cardiovascular
    Cell Biology
    Epigenetics
    Metabolism
    Developmental Biology
    By research area
    Immunology
    Microbiology
    Neuroscience
    Signal Transduction
    Stem Cells
  • Customized Products & Partnerships
    Customized Products & Partnerships

    Customized products and commercial partnerships to accelerate your diagnostic and therapeutic programs.

    Customized products

    Partner with us

  • Support
    Support hub

    Access advice and support for any research roadblock

    View support hub

    Protocols

    Your experiments laid out step by step

    View protocols

  • Events
    • Conference calendar
    • Cancer
    • Cardiovascular
    • Epigenetics & Nuclear signaling
    • Immunology
    • Neuroscience
    • Stem cells
    • Tradeshows
    • Scientific webinars
    Keep up to date with the latest events

    Full event breakdown with abstracts, speakers, registration and more

    View global event calendar

  • Pathways
    Cell signalling pathways

    View all pathways

    View all interactive pathways

Explore the power of knock-out cell lines for your research

  1. Link

    kdm5c--jarid1c--smcx--kdm5d--jarid1d--smcy-antibody-epr18653-chip-grade-ab194288.pdf

  1. Send me a copy of this email
    I agree to the terms and conditions.
Epigenetics and Nuclear Signaling Nuclear Signaling Pathways Nuclear Receptors Co-activators/co-repressors
Share by email
RecombinantRabMAb

Recombinant Anti-KDM5C / Jarid1C / SMCX + KDM5D / Jarid1D / SMCY antibody [EPR18653] - ChIP Grade (ab194288)

  • Datasheet
  • Certificate of Compliance
Submit a review Submit a question

Product price, shipping and contact information

Currently unavailable

Sorry, we can't display this right now.

Please contact us to place your order, or try again later.

 

Loading size & price…

 

Shipping and order information

Shipping info

Promotion Information

Abpromise

Guaranteed product quality, expert customer support.

Find out more.

Western blot - Anti-KDM5C / Jarid1C / SMCX + KDM5D / Jarid1D / SMCY antibody [EPR18653] - ChIP Grade (ab194288)
  • ChIP - Anti-KDM5C / Jarid1C / SMCX + KDM5D / Jarid1D / SMCY antibody [EPR18653] - ChIP Grade (ab194288)
  • Immunoprecipitation - Anti-KDM5C / Jarid1C / SMCX + KDM5D / Jarid1D / SMCY antibody [EPR18653] - ChIP Grade (ab194288)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-KDM5C / Jarid1C / SMCX + KDM5D / Jarid1D / SMCY antibody [EPR18653] - ChIP Grade (ab194288)
  • Western blot - Anti-KDM5C / Jarid1C / SMCX + KDM5D / Jarid1D / SMCY antibody [EPR18653] - ChIP Grade (ab194288)
  • Western blot - Anti-KDM5C / Jarid1C / SMCX + KDM5D / Jarid1D / SMCY antibody [EPR18653] - ChIP Grade (ab194288)
  • Immunoprecipitation - Anti-KDM5C / Jarid1C / SMCX + KDM5D / Jarid1D / SMCY antibody [EPR18653] - ChIP Grade (ab194288)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-KDM5C / Jarid1C / SMCX + KDM5D / Jarid1D / SMCY antibody [EPR18653] - ChIP Grade (ab194288)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-KDM5C / Jarid1C / SMCX + KDM5D / Jarid1D / SMCY antibody [EPR18653] - ChIP Grade (ab194288)
  • Anti-KDM5C / Jarid1C / SMCX + KDM5D / Jarid1D / SMCY antibody [EPR18653] - ChIP Grade (ab194288)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR18653] to KDM5C / Jarid1C / SMCX + KDM5D / Jarid1D / SMCY - ChIP Grade
  • Suitable for: IP, ChIP, WB, IHC-P
  • Reacts with: Mouse, Rat, Human, Recombinant fragment

Conjugates logo Related conjugates and formulations

Carrier Free

You may also be interested in

Secondary
Product image
Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
Knockout
Product image
Human KDM5C (Jarid1C / SMCX) knockout HEK-293T cell lysate (ab257494)
Knockout
Product image
Human KDM5C (Jarid1C / SMCX) knockout HEK-293T cell line (ab266251)

View more associated products

Overview

  • Product name

    Anti-KDM5C / Jarid1C / SMCX + KDM5D / Jarid1D / SMCY antibody [EPR18653] - ChIP Grade
  • Description

    Rabbit monoclonal [EPR18653] to KDM5C / Jarid1C / SMCX + KDM5D / Jarid1D / SMCY - ChIP Grade
  • Host species

    Rabbit
  • Tested applications

    Suitable for: IP, ChIP, WB, IHC-Pmore details
  • Species reactivity

    Reacts with: Mouse, Rat, Human, Recombinant fragment
  • Immunogen

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: His-tagged human KDM5D recombinant protein fragment, aa1-200; His-tagged human KDM5C recombinant protein fragment, aa1-200; Jurkat, HeLa, HEL, MCF7, PC-12, NIH/3T3 and F9 whole cell lysates. IP: HeLa and F9 whole cell lysates. ChIP: Chromatin from HeLa cells. IHC-P: Human and rat stomach tissue. Mouse cerebrum tissue.
  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.2
    Preservative: 0.01% Sodium azide
    Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Monoclonal
  • Clone number

    EPR18653
  • Isotype

    IgG
  • Research areas

    • Epigenetics and Nuclear Signaling
    • Nuclear Signaling Pathways
    • Nuclear Receptors
    • Co-activators/co-repressors
    • Epigenetics and Nuclear Signaling
    • Transcription
    • Other factors
    • Epigenetics and Nuclear Signaling
    • Transcription
    • Co-factors
    • Epigenetics and Nuclear Signaling
    • Chromatin Modifying Enzymes
    • Jumonji containing proteins
    • Epigenetics and Nuclear Signaling
    • ChIP assays
    • ChIP antibodies

Associated products

  • Alternative Versions

    • Anti-KDM5C / Jarid1C / SMCX + KDM5D / Jarid1D / SMCY antibody [EPR18653] - BSA and Azide free (ab232318)
  • Compatible Secondaries

    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
    • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
    • Goat Anti-Rabbit IgG H&L (HRP) (ab97051)
  • Isotype control

    • Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730)
  • Related Products

    • VeriBlot for IP Detection Reagent (HRP) (ab131366)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab194288 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IP
1/50.
ChIP
Use 5 µg for 25 µg of chromatin.
WB
1/1000. Detects a band of approximately 174 kDa (predicted molecular weight: 174 kDa).
IHC-P
1/100 - 1/1000.
Notes
IP
1/50.
ChIP
Use 5 µg for 25 µg of chromatin.
WB
1/1000. Detects a band of approximately 174 kDa (predicted molecular weight: 174 kDa).
IHC-P
1/100 - 1/1000.

Target

  • Cellular localization

    KDM5C / Jarid1C / SMCX: Nucleus. KDM5D / Jarid1D / SMCY: Nucleus.
  • Database links

    • Entrez Gene: 8242 Human
    • Entrez Gene: 8284 Human
    • Entrez Gene: 20591 Mouse
    • Entrez Gene: 20592 Mouse
    • Entrez Gene: 317432 Rat
    • Omim: 314690 Human
    • Omim: 426000 Human
    • SwissProt: P41229 Human
    • SwissProt: Q9BY66 Human
    • SwissProt: P41230 Mouse
    • SwissProt: Q62240 Mouse
    • Unigene: 631768 Human
    • Unigene: 80358 Human
    • Unigene: 142655 Mouse
    • Unigene: 262676 Mouse
    see all

Images

  • Western blot - Anti-KDM5C / Jarid1C / SMCX + KDM5D / Jarid1D / SMCY antibody [EPR18653] - ChIP Grade (ab194288)
    Western blot - Anti-KDM5C / Jarid1C / SMCX + KDM5D / Jarid1D / SMCY antibody [EPR18653] - ChIP Grade (ab194288)
    All lanes : Anti-KDM5C / Jarid1C / SMCX + KDM5D / Jarid1D / SMCY antibody [EPR18653] - ChIP Grade (ab194288) at 1/1000 dilution

    Lane 1 : Jurkat (Human T cell leukemia cell line from peripheral blood) whole cell lysate
    Lane 2 : HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell lysate
    Lane 3 : HEL (Human bone marrow erythroleukemia cell line) whole cell lysate
    Lane 4 : MCF7 (Human breast adenocarcinoma cell line) whole cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

    Predicted band size: 174 kDa
    Observed band size: 174 kDa


    Exposure time: 3 minutes


    Blocking/Dilution buffer: 5% NFDM/TBST.

    WB detection of KDM5C + KDM5D in tissue lysates may need optimization. In our hands this antibody could not detect KDM5C + KDM5D in human tissue lysates in WB.

  • ChIP - Anti-KDM5C / Jarid1C / SMCX + KDM5D / Jarid1D / SMCY antibody [EPR18653] - ChIP Grade (ab194288)
    ChIP - Anti-KDM5C / Jarid1C / SMCX + KDM5D / Jarid1D / SMCY antibody [EPR18653] - ChIP Grade (ab194288)

    Chromatin was prepared from HeLa (Human epithelial cell line from cervix adenocarcinoma) cells according to the Abcam X-ChIP protocol. Cells were fixed with formaldehyde for 10min. The ChIP was performed with 25μg of chromatin, 5μg of ab194288 (blue), and 20μl of Anti-rabbit IgG sepharose beads. 5µg of rabbit normal IgG was added to the beads control (yellow). The immunoprecipitated DNA was quantified by real time PCR (SYBR approach). Primers are located in the first kb of the transcribed region.

  • Immunoprecipitation - Anti-KDM5C / Jarid1C / SMCX + KDM5D / Jarid1D / SMCY antibody [EPR18653] - ChIP Grade (ab194288)
    Immunoprecipitation - Anti-KDM5C / Jarid1C / SMCX + KDM5D / Jarid1D / SMCY antibody [EPR18653] - ChIP Grade (ab194288)

    KDM5D + KDM5C was immunoprecipitated from 0.35 mg of F9 (Mouse embryonic testicular cancer cell line) whole cell lysate with ab194288 at 1/50 dilution. Western blot was performed from the immunoprecipitate using ab194288 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/10000 dilution.

    Lane 1: F9 whole cell lysate 10 μg (Input).

    Lane 2: ab194288 IP in F9 whole cell lysate.

    Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab194288 in F9 whole cell lysate.

    Blocking and dilution buffer and concentration: 5% NFDM/TBST.

    Exposure time: 3 minutes.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-KDM5C / Jarid1C / SMCX + KDM5D / Jarid1D / SMCY antibody [EPR18653] - ChIP Grade (ab194288)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-KDM5C / Jarid1C / SMCX + KDM5D / Jarid1D / SMCY antibody [EPR18653] - ChIP Grade (ab194288)

    Immunohistochemical analysis of paraffin-embedded Human stomach tissue labeling KDM5C / Jarid1C / SMCX+KDM5D / Jarid1D / SMCY with ab194288 at 1/100 dilution followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Nuclear staining on human stomach. The section was incubated with ab194288 for 30mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

    Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

  • Western blot - Anti-KDM5C / Jarid1C / SMCX + KDM5D / Jarid1D / SMCY antibody [EPR18653] - ChIP Grade (ab194288)
    Western blot - Anti-KDM5C / Jarid1C / SMCX + KDM5D / Jarid1D / SMCY antibody [EPR18653] - ChIP Grade (ab194288)
    All lanes : Anti-KDM5C / Jarid1C / SMCX + KDM5D / Jarid1D / SMCY antibody [EPR18653] - ChIP Grade (ab194288) at 1/5000 dilution

    Lane 1 : His-tagged human KDM5D recombinant protein fragment, aa1-200
    Lane 2 : His-tagged human KDM5C recombinant protein fragment, aa1-200

    Lysates/proteins at 0.01 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

    Predicted band size: 174 kDa


    Exposure time: 1 second


    Blocking/Dilution buffer: 5% NFDM/TBST.

    The predicted molecular weight of KDM5D recombinant protein fragment is 24.5 kDa. The predicted molecular weight of KDM5C recombinant protein fragment is 25 kDa

  • Western blot - Anti-KDM5C / Jarid1C / SMCX + KDM5D / Jarid1D / SMCY antibody [EPR18653] - ChIP Grade (ab194288)
    Western blot - Anti-KDM5C / Jarid1C / SMCX + KDM5D / Jarid1D / SMCY antibody [EPR18653] - ChIP Grade (ab194288)
    All lanes : Anti-KDM5C / Jarid1C / SMCX + KDM5D / Jarid1D / SMCY antibody [EPR18653] - ChIP Grade (ab194288) at 1/1000 dilution

    Lane 1 : PC-12 (Rat adrenal gland pheochromocytoma cell line) whole cell lysate
    Lane 2 : NIH/3T3 (Mouse embryonic fibroblast cell line) whole cell lysate
    Lane 3 : F9 (Mouse embryonic testicular cancer cell line) whole cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

    Predicted band size: 174 kDa
    Observed band size: 174 kDa



    Blocking/Dilution buffer: 5% NFDM/TBST.

    Exposure time: Lane 1 and 2: 3 minutes; Lane 3: 15 seconds.

    WB detection of KDM5C + KDM5D in tissue lysates may need optimization. In our hands this antibody could not detect KDM5C + KDM5D in mouse and rat tissue lysates in WB.

  • Immunoprecipitation - Anti-KDM5C / Jarid1C / SMCX + KDM5D / Jarid1D / SMCY antibody [EPR18653] - ChIP Grade (ab194288)
    Immunoprecipitation - Anti-KDM5C / Jarid1C / SMCX + KDM5D / Jarid1D / SMCY antibody [EPR18653] - ChIP Grade (ab194288)

    KDM5D + KDM5C was immunoprecipitated from 0.35 mg of HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell lysate with ab194288 at 1/50 dilution. Western blot was performed from the immunoprecipitate using ab194288 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/10000 dilution.

    Lane 1: HeLa whole cell lysate 10 μg (Input).

    Lane 2: ab194288 IP in HeLa whole cell lysate.

    Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab194288 in HeLa whole cell lysate.

    Blocking and dilution buffer and concentration: 5% NFDM/TBST.

    Exposure time: 3 minutes.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-KDM5C / Jarid1C / SMCX + KDM5D / Jarid1D / SMCY antibody [EPR18653] - ChIP Grade (ab194288)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-KDM5C / Jarid1C / SMCX + KDM5D / Jarid1D / SMCY antibody [EPR18653] - ChIP Grade (ab194288)

    Immunohistochemical analysis of paraffin-embedded Rat stomach tissue labeling KDM5C / Jarid1C / SMCX+KDM5D / Jarid1D / SMCY with ab194288 at 1/1000 dilution followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Nuclear staining on rat stomach. The section was incubated with ab194288 for 30mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

    Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-KDM5C / Jarid1C / SMCX + KDM5D / Jarid1D / SMCY antibody [EPR18653] - ChIP Grade (ab194288)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-KDM5C / Jarid1C / SMCX + KDM5D / Jarid1D / SMCY antibody [EPR18653] - ChIP Grade (ab194288)

    Immunohistochemical analysis of paraffin-embedded Mouse cerebrum tissue labeling KDM5C / Jarid1C / SMCX+KDM5D / Jarid1D / SMCY with ab194288 at 1/1000 dilution followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Nuclear staining on mouse cerebrum. The section was incubated with ab194288 for 30mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

    Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

  • Anti-KDM5C / Jarid1C / SMCX + KDM5D / Jarid1D / SMCY antibody [EPR18653] - ChIP Grade (ab194288)
    Anti-KDM5C / Jarid1C / SMCX + KDM5D / Jarid1D / SMCY antibody [EPR18653] - ChIP Grade (ab194288)

Protocols

  • ChIP protocols
  • Western blot protocols
  • Immunoprecipitation protocols

Click here to view the general protocols

Datasheets and documents

  • Datasheet download

    Download

Certificate of Compliance

To download a Certificate of Compliance, please enter your Lot number below:

References (0)

Publishing research using ab194288? Please let us know so that we can cite the reference in this datasheet.

ab194288 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

Show All Reviews Q&A
Submit a review Submit a question

There are currently no Customer reviews or Questions for ab194288.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

Get resources and offers direct to your inbox Sign up
A-Z by research area
  • Cancer
  • Cardiovascular
  • Cell biology
  • Developmental biology
  • Epigenetics & Nuclear signaling
  • Immunology
  • Metabolism
  • Microbiology
  • Neuroscience
  • Signal transduction
  • Stem cells
A-Z by product type
  • Primary antibodies
  • Secondary antibodies
  • Biochemicals
  • Isotype controls
  • Flow cytometry multi-color selector
  • Kits
  • Loading controls
  • Lysates
  • Peptides
  • Proteins
  • Slides
  • Tags and cell markers
  • Tools & Reagents
Help & support
  • Support
  • Make an Inquiry
  • Protocols & troubleshooting
  • Placing an order
  • RabMAb products
  • Biochemical product FAQs
  • Training
  • Browse by Target
Company
  • Corporate site
  • Investor relations
  • Company news
  • Careers
  • About us
  • Blog
Events
  • Tradeshows
  • Conferences
International websites
  • abcam.cn
  • abcam.co.jp

Join with us

  • LinkedIn
  • facebook
  • Twitter
  • YouTube
  • Terms of sale
  • Website terms of use
  • Cookie policy
  • Privacy policy
  • Legal
  • Modern slavery statement
© 1998-2022 Abcam plc. All rights reserved.