Anti-KDM5C / Jarid1C / SMCX antibody (ab34718)


  • Product name
    Anti-KDM5C / Jarid1C / SMCX antibody
    See all KDM5C / Jarid1C / SMCX primary antibodies
  • Description
    Rabbit polyclonal to KDM5C / Jarid1C / SMCX
  • Host species
  • Tested applications
    Suitable for: ChIP, IHC-FoFr, WB, ICC/IFmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Pig
  • Immunogen

    Synthetic peptide conjugated to KLH derived from within residues 1500 to the C-terminus of Human Jarid1C/ SMCX.

    (Peptide available as ab35501.)

  • Positive control
    • This antibody gave a positive signal in HEK 293 (Human embryonic kidney cell line), and Y79 (Human retinoblastoma cell line) Whole Cell Lysates. This antibody gave a positive signal in the following Methanol fixed cell lines:HeLa, Hek293, HepG2, and MCF-7. This antibody gave a positive signal in the following Formaldehyde fixed cell lines:HepG2, and MCF-7.



Our Abpromise guarantee covers the use of ab34718 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ChIP Use at an assay dependent concentration. PubMed: 19136938
IHC-FoFr Use at an assay dependent concentration.
WB 1/250. Detects a band of approximately 180 kDa (predicted molecular weight: 176 kDa).

Abcam recommends using milk as the blocking agent.

ICC/IF Use a concentration of 1 µg/ml.


  • Function
    Histone demethylase that specifically demethylates 'Lys-4' of histone H3, thereby playing a central role in histone code. Does not demethylate histone H3 'Lys-9', H3 'Lys-27', H3 'Lys-36', H3 'Lys-79' or H4 'Lys-20'. Demethylates trimethylated and dimethylated but not monomethylated H3 'Lys-4'. Participates in transcriptional repression of neuronal genes by recruiting histone deacetylases and REST at neuron-restrictive silencer elements.
  • Tissue specificity
    Expressed in all tissues examined. Highest levels found in brain and skeletal muscle.
  • Involvement in disease
    Defects in KDM5C are the cause of mental retardation syndromic X-linked JARID1C-related (MRXSJ) [MIM:300534]. MRXSJ is characterized by significantly sub-average general intellectual functioning associated with impairments in adaptative behavior and manifested during the developmental period. MRXSJ patients manifest mental retardation associated with variable features such as slowly progressive spastic paraplegia, seizures, facial dysmorphism.
  • Sequence similarities
    Belongs to the JARID1 histone demethylase family.
    Contains 1 ARID domain.
    Contains 1 JmjC domain.
    Contains 1 JmjN domain.
    Contains 2 PHD-type zinc fingers.
  • Domain
    The first PHD-type zinc finger domain recognizes and binds H3-K9Me3.
    Both the JmjC domain and the JmjN domain are required for enzymatic activity.
  • Cellular localization
  • Information by UniProt
  • Database links
  • Alternative names
    • DXS1272E antibody
    • Histone demethylase JARID1C antibody
    • JARID1C antibody
    • JmjC domain containing protein SMCX antibody
    • Jumonji AT rich interactive domain 1C antibody
    • Jumonji, AT rich interactive domain 1C (RBP2 like) antibody
    • Jumonji/ARID domain-containing protein 1C antibody
    • KDM5C antibody
    • KDM5C_HUMAN antibody
    • Lysine (K) specific demethylase 5C antibody
    • Lysine-specific demethylase 5C antibody
    • MRX13 antibody
    • MRXJ antibody
    • MRXSCJ antibody
    • MRXSJ antibody
    • Protein SmcX antibody
    • Protein Xe169 antibody
    • rbp2 like protein antibody
    • Selected cDNA on X antibody
    • SMCX antibody
    • Smcx homolog X chromosome antibody
    • SmcX protein antibody
    • Smcy homolog X linked antibody
    • XE169 antibody
    • Xe169 protein antibody
    see all


  • Lane 1: Wild-type HAP1 whole cell lysate (20 µg)
    Lane 2: KDM5C knockout HAP1 whole cell lysate (20 µg)
    Lane 3: HEK293 whole cell lysate (20 µg)
    Lane 4: U2OS whole cell lysate (20 µg)

    Lanes 1 - 4: Merged signal (red and green). Green - ab34718 observed at 175 kDa. Red - loading control, ab18058, observed at 120 kDa.

    ab34718 was shown to specifically recognize KDM5C in wild-type HAP1 cells as signal was lost at the expected MW in KDM5C knockout cells. Additional cross-reactive bands were observed in the wild-type and knockout cells. Wild-type and KDM5C knockout samples were subjected to SDS-PAGE. Ab34718 and ab18058 (Mouse anti Vinculin loading control) were incubated overnight at 4°C at 1/250 dilution and 1/20,000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed (ab216776) secondary antibodies at 1/20,000 dilution for 1 hour at room temperature before imaging.

  • PFA-fixed, 0.5% Triton X-100 permeabilized HeLa (human epithelial cell line from cervix adenocarcinoma) cells stained for KDM5C / Jarid1C / SMCX (green) using ab34718 at 1/200 dilution in ICC/IF. Counter-stained with DAPI in order to highlight the nucleus (red). Please refer to abreview for further experimental details.

    See Abreview

  • ab34718 stained in Hela cells. Cells were fixed with 100% methanol (5min) at room temperature and incubated with PBS containing 10% goat serum, 0.3 M glycine, 1% BSA and 0.1% triton for 1h at room temperature to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab34718 at 5µg/ml and ab7291 (Mouse monoclonal [DM1A] to alpha Tubulin - Loading Control) at 1/1000 dilution overnight at +4°C. The secondary antibodies were ab150120 (pseudo-colored red) and ab150081 (colored green) used at 1 µg/ml for 1 hour at room temperature. DAPI was used to stain the cell nuclei (colored blue) at a concentration of 1.43 µM for 1hour at room temperature.

  • All lanes : Anti-KDM5C / Jarid1C / SMCX antibody (ab34718) at 1 µg/ml

    Lane 1 : HEK293 (Human) Whole Cell Lysate
    Lane 2 : Y79 (Human retinoblastoma cell line) Whole Cell Lysate

    Lysates/proteins at 20 µg per lane.

    All lanes : Goat Anti-Rabbit IgG H&L (HRP) preadsorbed at 1/50000 dilution

    Developed using the ECL technique.

    Performed under reducing conditions.

    Predicted band size: 176 kDa
    Observed band size: 180 kDa (why is the actual band size different from the predicted?)
    Additional bands at: 45 kDa, 58 kDa. We are unsure as to the identity of these extra bands.

    Exposure time: 20 minutes

    This blot was produced using a 3-8% Tris Acetate gel under the TA buffer system. The gel was run at 150V for 60 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 3% Milk before being incubated with ab34718 overnight at 4°C. Antibody binding was detected using an anti-rabbit antibody conjugated to HRP, and visualised using ECL development solution ab133406.

  • Anti-KDM5C / Jarid1C / SMCX antibody (ab34718) at 1/250 dilution + HEK293 whole cell lysate (ab7902) at 20 µg

    IRDye 680 Conjugated Goat Anti-Rabbit IgG (H+L) at 1/10000 dilution

    Performed under reducing conditions.

    Predicted band size: 176 kDa
    Observed band size: 176 kDa


This product has been referenced in:
  • Fujita T  et al. Identification of telomere-associated molecules by engineered DNA-binding molecule-mediated chromatin immunoprecipitation (enChIP). Sci Rep 3:3171 (2013). ICC/IF ; Human . Read more (PubMed: 24201379) »
  • Liang Z  et al. Proliferating cell nuclear antigen is required for loading of the SMCX/KMD5C histone demethylase onto chromatin. Epigenetics Chromatin 4:18 (2011). Read more (PubMed: 21996408) »

See all 4 Publications for this product

Customer reviews and Q&As

Immunocytochemistry/ Immunofluorescence
Human Cell (HeLa)
Yes - 0.5% Triton-X100 in PBS

Dr. Kirk Mcmanus

Verified customer

Submitted Feb 27 2015

Western blot
Human Cell lysate - whole cell (different cell lines)
Gel Running Conditions
Reduced Denaturing (7,5% gel)
Loading amount
50 µg
different cell lines
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C

Abcam user community

Verified customer

Submitted Nov 29 2013

Thank you for contacting us.

I have checked the homology between KDM5D and KDM5C, and for the immunogen region of ab34718, the homology is poor and the antibody would not cross react.

Unfortunately, we do not carry antibodies that ...

Read More
Immunohistochemistry (PFA perfusion fixed frozen sections)
Mouse Tissue sections (brain-perfused frozen sections)
brain-perfused frozen sections

Abcam user community

Verified customer

Submitted May 30 2012

Thank you for contacting us.

The abreview (xxx) is in dormant status as it was not edited for over 28 days. I have just put it back into editing status and you should be able to see it. Lots of information is still missing which would need t...

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Thank you for your email.
I am glad to hear that the antibody has worked successfully in the untested species.

Here iswhat you need to do in order to activate your discount code:

The code will give you 1 free PRIMARY ANTI...

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Thank you for confirming these details and for your cooperation. The details provided enable us to closely monitor the quality of our products.

I am sorry this product did not perform as stated on the datasheet and for the inconvenience this...

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Abcam guarantees this product to work in the species/application used in this Abreview.
Western blot
Human Cell lysate - whole cell (Y79 retinoblastoma cell line)
Gel Running Conditions
Reduced Denaturing (10%)
Loading amount
10 µg
Y79 retinoblastoma cell line
Blocking step
BSA as blocking agent for 12 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 4°C

Abcam user community

Verified customer

Submitted Sep 12 2008


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