Thank you for submitting your Abreview. You may be able to reduce your background by adding a blocking step with 10% normal goat serum prior to your primary antibody incubation and using less secondary antibody (1:1000-1:5000). The serum block should reduce most of your background, but you can also add an H2O2 block (such as ab94666) for endogenous peroxidases and / or an avidin biotin block (such as ab64212) if these are not included in your ABC kit.
Abcam user community
Submitted May 30 2012
Get resources and offers direct to your inboxSign up