Immunohistochemistry (PFA perfusion fixed frozen sections) abreview for Anti-KDM5C / Jarid1C / SMCX antibody

Good
Abreviews
Application
Immunohistochemistry (PFA perfusion fixed frozen sections)
Sample
Mouse Tissue sections (brain-perfused frozen sections)
Specification
brain-perfused frozen sections
Fixative
Paraformaldehyde

Other product details

Incubation time
16 hour(s) and 0 minute(s)
Dilution
1/1000

Secondary antibody

Dilution
1/500
Name
Non-Abcam antibody was used: biotinylated goat anti-rabbit immunoglobulinG Vect
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase

Additional data

Abcam response
Thank you for submitting your Abreview. You may be able to reduce your background by adding a blocking step with 10% normal goat serum prior to your primary antibody incubation and using less secondary antibody (1:1000-1:5000). The serum block should reduce most of your background, but you can also add an H2O2 block (such as ab94666) for endogenous peroxidases and / or an avidin biotin block (such as ab64212) if these are not included in your ABC kit.

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Submitted May 30 2012

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