We used different cell lines, total extracts (nuclear + cytosolic), that should be positive for JARID1c.
Several unspecific bands, very difficult to detect the protein. We get a band around the expected molecular weight but it is difficult to trust since we have dozens of other bands throughout the membrane.
if we use more diluted ab, no bands are detected.
We used different cell lines, ab dilutions, exposure times etc…
lysis buffer: RIPA + protease inhibitors (Roche)
We appreciate the valuable data provided in this Abreview. Since the antibody didn't work as it was supposed to in our previously tested and guaranteed species and application, we have followed up with the customer in accordance to our Abpromise (www.abcam.com/abpromise) and issued a replacement.
Abcam user community
Submitted Nov 29 2013
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