Asynchronous cells were paraformaldehyde-fixed, permeablized, immunofluorescently labeled and counterstained with DAPI. This antibody produces a focal staining pattern that is highly enriched within interphase nuclei. MeOH-fixed samples were also evaluated, but failed to produce a similar strong, nuclear-enriched staining pattern.
Dr. Kirk Mcmanus
Submitted Feb 27 2015
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