Immunocytochemistry/ Immunofluorescence abreview for Anti-KDM5C / Jarid1C / SMCX antibody

Excellent
Abreviews
Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (HeLa)
Permeabilization
Yes - 0.5% Triton-X100 in PBS
Specification
HeLa
Fixative
Paraformaldehyde

Other product details

Incubation time
16 hour(s) and 0 minute(s) · Temperature: 22°C · Diluent: PBS
Dilution
1/200

Secondary antibody

Dilution
1/200

Additional data

Additional Notes
Asynchronous cells were paraformaldehyde-fixed, permeablized, immunofluorescently labeled and counterstained with DAPI. This antibody produces a focal staining pattern that is highly enriched within interphase nuclei. MeOH-fixed samples were also evaluated, but failed to produce a similar strong, nuclear-enriched staining pattern.

Dr. Kirk Mcmanus

Verified customer

Submitted Feb 27 2015

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