For the best experience on the Abcam website please upgrade to a modern browser such as Google Chrome

Hello. We're improving abcam.com and we'd welcome your feedback.

Hello. We're improving abcam.com and we'd welcome your feedback.

Infomation icon

We haven't added this to the BETA yet

New BETA website

New BETA website

Hello. We're improving abcam.com and we'd welcome your feedback.

Take a look at our BETA site and see what we’ve done so far.

Switch on our new BETA site

Now available

Search and browse selected products

  • A selection of primary antibodies

Purchase these through your usual distributor

In the coming months

  • Additional product types
  • Supporting content
  • Sign in to your account
  • Purchase online
United States
Your country/region is currently set to:

If incorrect, please enter your country/region into the box below, to view site information related to your country/region.

Call (888) 77-ABCAM (22226) or contact us
Need help? Contact us

  • My account
  • Sign out
Sign in or Register with us

Welcome

Sign in or

Don't have an account?

Register with us
My basket
Quick order
Abcam homepage

  • Research Products
    By product type
    Primary antibodies
    Secondary antibodies
    ELISA and Matched Antibody Pair Kits
    Cell and tissue imaging tools
    Cellular and biochemical assays
    Proteins and Peptides
    By product type
    Proteomics tools
    Agonists, activators, antagonists and inhibitors
    Cell lines and Lysates
    Multiplex miRNA assays
    Multiplex Assays
    By research area
    Cancer
    Cardiovascular
    Cell Biology
    Epigenetics
    Metabolism
    Developmental Biology
    By research area
    Immunology
    Microbiology
    Neuroscience
    Signal Transduction
    Stem Cells
  • Customized Products & Partnerships
    Customized Products & Partnerships

    Customized products and commercial partnerships to accelerate your diagnostic and therapeutic programs.

    Customized products

    Partner with us

  • Support
    Support hub

    Access advice and support for any research roadblock

    View support hub

    Protocols

    Your experiments laid out step by step

    View protocols

  • Events
    • Conference calendar
    • Cancer
    • Cardiovascular
    • Epigenetics & Nuclear signaling
    • Immunology
    • Neuroscience
    • Stem cells
    • Tradeshows
    • Scientific webinars
    Keep up to date with the latest events

    Full event breakdown with abstracts, speakers, registration and more

    View global event calendar

  • Pathways
    Cell signalling pathways

    View all pathways

    View all interactive pathways

Supporting our customers and employees during the COVID-19 pandemic. Read more

  1. Link

    kdm5c--jarid1c--smcx-antibody-epr23932-18-ab259913.pdf

  1. Send me a copy of this email
    I agree to the terms and conditions.
Epigenetics and Nuclear Signaling Nuclear Signaling Pathways Nuclear Receptors Co-activators/co-repressors
Share by email
Validated using a knockout cell lineRecombinantRabMAb

Recombinant Anti-KDM5C / Jarid1C / SMCX antibody [EPR23932-18] (ab259913)

  • Datasheet
  • Certificate of Compliance
Submit a review Submit a question

Product price, shipping and contact information

Currently unavailable

Sorry, we can't display this right now.

Please contact us to place your order, or try again later.

 

Loading size & price…

 

Shipping and order information

Shipping info

Promotion Information

Abpromise

Guaranteed product quality, expert customer support.

Find out more.

Western blot - Anti-KDM5C / Jarid1C / SMCX antibody [EPR23932-18] (ab259913)
  • Western blot - Anti-KDM5C / Jarid1C / SMCX antibody [EPR23932-18] (ab259913)
  • Western blot - Anti-KDM5C / Jarid1C / SMCX antibody [EPR23932-18] (ab259913)
  • Anti-KDM5C / Jarid1C / SMCX antibody [EPR23932-18] (ab259913)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR23932-18] to KDM5C / Jarid1C / SMCX
  • Suitable for: WB
  • Knockout validated
  • Reacts with: Human

Conjugates logo Related conjugates and formulations

Carrier Free

You may also be interested in

Primary
Product image
Anti-Smad1 (phospho S463 + S465) antibody [EPR20662-29] (ab214423)
Knockout
Product image
Human KDM5C (Jarid1C / SMCX) knockout HEK-293T cell line (ab266251)
Knockout
Product image
Human KDM5C (Jarid1C / SMCX) knockout HEK-293T cell line (ab266252)

View more associated products

Overview

  • Product name

    Anti-KDM5C / Jarid1C / SMCX antibody [EPR23932-18]
    See all KDM5C / Jarid1C / SMCX primary antibodies
  • Description

    Rabbit monoclonal [EPR23932-18] to KDM5C / Jarid1C / SMCX
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WBmore details
    Unsuitable for: ChIP,Flow Cyt (Intra),ICC,IHC-P or IP
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: HEK293T, HeLa, HAP1, NCCIT.
  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    Preservative: 0.01% Sodium azide
    Constituents: 59.94% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Monoclonal
  • Clone number

    EPR23932-18
  • Isotype

    IgG
  • Research areas

    • Epigenetics and Nuclear Signaling
    • Nuclear Signaling Pathways
    • Nuclear Receptors
    • Co-activators/co-repressors
    • Epigenetics and Nuclear Signaling
    • Transcription
    • Other factors
    • Epigenetics and Nuclear Signaling
    • Transcription
    • Co-factors
    • Epigenetics and Nuclear Signaling
    • Chromatin Modifying Enzymes
    • Jumonji containing proteins

Associated products

  • Alternative Versions

    • Anti-KDM5C / Jarid1C / SMCX antibody [EPR23932-18] - BSA and Azide free (ab280963)
  • KO cell lines

    • Human KDM5C (Jarid1C / SMCX) knockout HEK-293T cell line (ab266251)
    • Human KDM5C (Jarid1C / SMCX) knockout HEK-293T cell line (ab266252)
  • KO cell lysates

    • Human KDM5C (Jarid1C / SMCX) knockout HEK-293T cell lysate (ab257494)
    • Human KDM5C (Jarid1C / SMCX) knockout HEK-293T cell lysate (ab257495)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab259913 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
1/1000. Predicted molecular weight: 175 kDa.
Notes
WB
1/1000. Predicted molecular weight: 175 kDa.
Application notes
Is unsuitable for ChIP,Flow Cyt (Intra),ICC,IHC-P or IP.

Target

  • Function

    Histone demethylase that specifically demethylates 'Lys-4' of histone H3, thereby playing a central role in histone code. Does not demethylate histone H3 'Lys-9', H3 'Lys-27', H3 'Lys-36', H3 'Lys-79' or H4 'Lys-20'. Demethylates trimethylated and dimethylated but not monomethylated H3 'Lys-4'. Participates in transcriptional repression of neuronal genes by recruiting histone deacetylases and REST at neuron-restrictive silencer elements.
  • Tissue specificity

    Expressed in all tissues examined. Highest levels found in brain and skeletal muscle.
  • Involvement in disease

    Defects in KDM5C are the cause of mental retardation syndromic X-linked JARID1C-related (MRXSJ) [MIM:300534]. MRXSJ is characterized by significantly sub-average general intellectual functioning associated with impairments in adaptative behavior and manifested during the developmental period. MRXSJ patients manifest mental retardation associated with variable features such as slowly progressive spastic paraplegia, seizures, facial dysmorphism.
  • Sequence similarities

    Belongs to the JARID1 histone demethylase family.
    Contains 1 ARID domain.
    Contains 1 JmjC domain.
    Contains 1 JmjN domain.
    Contains 2 PHD-type zinc fingers.
  • Domain

    The first PHD-type zinc finger domain recognizes and binds H3-K9Me3.
    Both the JmjC domain and the JmjN domain are required for enzymatic activity.
  • Cellular localization

    Nucleus.
  • Target information above from: UniProt accession P41229 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 8242 Human
    • Omim: 314690 Human
    • SwissProt: P41229 Human
    • Unigene: 631768 Human
    • Alternative names

      • DXS1272E antibody
      • Histone demethylase JARID1C antibody
      • JARID1C antibody
      • JmjC domain containing protein SMCX antibody
      • Jumonji AT rich interactive domain 1C antibody
      • Jumonji, AT rich interactive domain 1C (RBP2 like) antibody
      • Jumonji/ARID domain-containing protein 1C antibody
      • KDM5C antibody
      • KDM5C_HUMAN antibody
      • Lysine (K) specific demethylase 5C antibody
      • Lysine-specific demethylase 5C antibody
      • MRX13 antibody
      • MRXJ antibody
      • MRXSCJ antibody
      • MRXSJ antibody
      • Protein SmcX antibody
      • Protein Xe169 antibody
      • rbp2 like protein antibody
      • Selected cDNA on X antibody
      • SMCX antibody
      • Smcx homolog X chromosome antibody
      • SmcX protein antibody
      • Smcy homolog X linked antibody
      • XE169 antibody
      • Xe169 protein antibody
      see all

    Images

    • Western blot - Anti-KDM5C / Jarid1C / SMCX antibody [EPR23932-18] (ab259913)
      Western blot - Anti-KDM5C / Jarid1C / SMCX antibody [EPR23932-18] (ab259913)
      All lanes : Anti-KDM5C / Jarid1C / SMCX antibody [EPR23932-18] (ab259913) at 1/1000 dilution

      Lane 1 : Wild-type HEK293T (human embryonic kidney epithelial cell), whole cell lysate
      Lane 2 : Human KDM5C (Jarid1C / SMCX) knockout HEK-293T cell line (ab266252)
      Lane 3 : Human KDM5C (Jarid1C / SMCX) knockout HEK-293T cell line (ab266251)
      Lane 4 : HeLa (human cervix adenocarcinoma epithelial cell), whole cell lysate

      Lysates/proteins at 20 µg per lane.

      Secondary
      All lanes : Goat Anti-Rabbit IgG H&L (IRDye® 800CW) (ab216773) and Goat Anti-Mouse IgG H&L (IRDye® 680RD) (ab216776)

      Predicted band size: 175 kDa
      Observed band size: 180 kDa why is the actual band size different from the predicted?



      Blocking and Diluting buffer and concentration: 5% NFDM/TBST

      Lanes 1-4: Merged signal (red and green). Green - ab259913 observed at 180 kDa. Red - loading control ab8245 (Mouse monoclonal [6C5] to GAPDH) observed at 36 kDa.

      ab259913 Anti-KDM5C / Jarid1C / SMCX antibody [EPR23932-18] was shown to specifically react with KDM5C / Jarid1C / SMCX in wild-type HEK293T cells. Loss of signal was observed when knockout cell line ab266251 (knockout cell lysate ab257494) and ab266252 (knockout cell lysate ab257495) were used. Wild-type and KDM5C / Jarid1C / SMCX knockout samples were subjected to SDS-PAGE. ab259913 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) were incubated at 4? overnight at 1 in 1000 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 10000 dilution for 1 hour at room temperature before imaging.

    • Western blot - Anti-KDM5C / Jarid1C / SMCX antibody [EPR23932-18] (ab259913)
      Western blot - Anti-KDM5C / Jarid1C / SMCX antibody [EPR23932-18] (ab259913)
      All lanes : Anti-KDM5C / Jarid1C / SMCX antibody [EPR23932-18] (ab259913) at 1/1000 dilution

      Lane 1 : HAP1 (human chronic myelogenous leukemia near-haploid cell), whole cell lysate
      Lane 2 : HEK293T (human embryonic kidney epithelial cell), whole cell lysate

      Lysates/proteins at 20 µg per lane.

      Secondary
      All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/50000 dilution (Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated)

      Predicted band size: 175 kDa
      Observed band size: 180 kDa why is the actual band size different from the predicted?



      Blocking and Diluting buffer and concentration: Intercept® (TBS) Blocking Buffer diluted with an equal volume of 0.1% TBS

      Exposure time 70 seconds

      Smaller bands are non-specific signal.

    • Western blot - Anti-KDM5C / Jarid1C / SMCX antibody [EPR23932-18] (ab259913)
      Western blot - Anti-KDM5C / Jarid1C / SMCX antibody [EPR23932-18] (ab259913)
      Anti-KDM5C / Jarid1C / SMCX antibody [EPR23932-18] (ab259913) at 1/2000 dilution + NCCIT (human pluripotent embryonic carcinoma epithelial cell) whole cell lysate at 20 µg

      Secondary
      Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution (Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated)

      Predicted band size: 175 kDa
      Observed band size: 180 kDa why is the actual band size different from the predicted?



      Blocking and Diluting buffer and concentration: 5% NFDM/TBST

      Exposure time 3 minutes

      Smaller bands are non-specific signal.

    • Anti-KDM5C / Jarid1C / SMCX antibody [EPR23932-18] (ab259913)
      Anti-KDM5C / Jarid1C / SMCX antibody [EPR23932-18] (ab259913)

    Protocols

    To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

    Click here to view the general protocols

    Datasheets and documents

    • Datasheet download

      Download

    Certificate of Compliance

    To download a Certificate of Compliance, please enter your Lot number below:

    References (0)

    Publishing research using ab259913? Please let us know so that we can cite the reference in this datasheet.

    ab259913 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

    Show All Reviews Q&A
    Submit a review Submit a question

    There are currently no Customer reviews or Questions for ab259913.
    Please use the links above to contact us or submit feedback about this product.

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
    For licensing inquiries, please contact partnerships@abcam.com

    Get resources and offers direct to your inbox Sign up
    A-Z by research area
    • Cancer
    • Cardiovascular
    • Cell biology
    • Developmental biology
    • Epigenetics & Nuclear signaling
    • Immunology
    • Metabolism
    • Microbiology
    • Neuroscience
    • Signal transduction
    • Stem cells
    A-Z by product type
    • Primary antibodies
    • Secondary antibodies
    • Biochemicals
    • Isotype controls
    • Flow cytometry multi-color selector
    • Kits
    • Loading controls
    • Lysates
    • Peptides
    • Proteins
    • Slides
    • Tags and cell markers
    • Tools & Reagents
    Help & support
    • Support
    • Make an Inquiry
    • Protocols & troubleshooting
    • Placing an order
    • RabMAb products
    • Biochemical product FAQs
    • Training
    • Browse by Target
    Company
    • Corporate site
    • Investor relations
    • Company news
    • Careers
    • About us
    • Blog
    Events
    • Tradeshows
    • Conferences
    International websites
    • abcam.cn
    • abcam.co.jp

    Join with us

    • LinkedIn
    • facebook
    • Twitter
    • YouTube
    • Terms of sale
    • Website terms of use
    • Cookie policy
    • Privacy policy
    • Legal
    • Modern slavery statement
    © 1998-2022 Abcam plc. All rights reserved.