Recombinant Anti-KDM5C / Jarid1C / SMCX antibody [EPR23932-18] (ab259913)
![Western blot - Anti-KDM5C / Jarid1C / SMCX antibody [EPR23932-18] (ab259913)](https://www.abcam.com/ps/products/259/ab259913/Images/ab259913-457452-antiKDM5C--Jarid1C--SMCX-antibody-EPR2393218-western-blot-ko-hek293t.jpg "Western blot - Anti-KDM5C / Jarid1C / SMCX antibody [EPR23932-18] (ab259913)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR23932-18] to KDM5C / Jarid1C / SMCX
- Suitable for: WB
- Knockout validated
- Reacts with: Human
Related conjugates and formulations
Overview
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Product name
Anti-KDM5C / Jarid1C / SMCX antibody [EPR23932-18]
See all KDM5C / Jarid1C / SMCX primary antibodies -
Description
Rabbit monoclonal [EPR23932-18] to KDM5C / Jarid1C / SMCX -
Host species
Rabbit -
Tested applications
Suitable for: WBmore details
Unsuitable for: ChIP,Flow Cyt (Intra),ICC,IHC-P or IP -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- WB: HEK293T, HeLa, HAP1, NCCIT.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.01% Sodium azide
Constituents: 59.94% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR23932-18 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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KO cell lines
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KO cell lysates
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab259913 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB |
1/1000. Predicted molecular weight: 175 kDa.
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| Notes |
|---|
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WB
1/1000. Predicted molecular weight: 175 kDa. |
Target
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Function
Histone demethylase that specifically demethylates 'Lys-4' of histone H3, thereby playing a central role in histone code. Does not demethylate histone H3 'Lys-9', H3 'Lys-27', H3 'Lys-36', H3 'Lys-79' or H4 'Lys-20'. Demethylates trimethylated and dimethylated but not monomethylated H3 'Lys-4'. Participates in transcriptional repression of neuronal genes by recruiting histone deacetylases and REST at neuron-restrictive silencer elements. -
Tissue specificity
Expressed in all tissues examined. Highest levels found in brain and skeletal muscle. -
Involvement in disease
Defects in KDM5C are the cause of mental retardation syndromic X-linked JARID1C-related (MRXSJ) [MIM:300534]. MRXSJ is characterized by significantly sub-average general intellectual functioning associated with impairments in adaptative behavior and manifested during the developmental period. MRXSJ patients manifest mental retardation associated with variable features such as slowly progressive spastic paraplegia, seizures, facial dysmorphism. -
Sequence similarities
Belongs to the JARID1 histone demethylase family.
Contains 1 ARID domain.
Contains 1 JmjC domain.
Contains 1 JmjN domain.
Contains 2 PHD-type zinc fingers. -
Domain
The first PHD-type zinc finger domain recognizes and binds H3-K9Me3.
Both the JmjC domain and the JmjN domain are required for enzymatic activity. -
Cellular localization
Nucleus. - Information by UniProt
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Database links
- Entrez Gene: 8242 Human
- Omim: 314690 Human
- SwissProt: P41229 Human
- Unigene: 631768 Human
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Alternative names
- DXS1272E antibody
- Histone demethylase JARID1C antibody
- JARID1C antibody
see all
Images
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Western blot - Anti-KDM5C / Jarid1C / SMCX antibody [EPR23932-18] (ab259913)All lanes : Anti-KDM5C / Jarid1C / SMCX antibody [EPR23932-18] (ab259913) at 1/1000 dilution
Lane 1 : Wild-type HEK293T (human embryonic kidney epithelial cell), whole cell lysate
Lane 2 :Human KDM5C (Jarid1C / SMCX) knockout HEK-293T cell line (ab266252)
Lane 3 :Human KDM5C (Jarid1C / SMCX) knockout HEK-293T cell line (ab266251)
Lane 4 : HeLa (human cervix adenocarcinoma epithelial cell), whole cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (IRDye® 800CW) (ab216773) and Goat Anti-Mouse IgG H&L (IRDye® 680RD) (ab216776)
Predicted band size: 175 kDa
Observed band size: 180 kDa why is the actual band size different from the predicted?Blocking and Diluting buffer and concentration: 5% NFDM/TBST
Lanes 1-4: Merged signal (red and green). Green - ab259913 observed at 180 kDa. Red - loading control ab8245 (Mouse monoclonal [6C5] to GAPDH) observed at 36 kDa.
ab259913 Anti-KDM5C / Jarid1C / SMCX antibody [EPR23932-18] was shown to specifically react with KDM5C / Jarid1C / SMCX in wild-type HEK293T cells. Loss of signal was observed when knockout cell line ab266251 (knockout cell lysate ab257494) and ab266252 (knockout cell lysate ab257495) were used. Wild-type and KDM5C / Jarid1C / SMCX knockout samples were subjected to SDS-PAGE. ab259913 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) were incubated at 4? overnight at 1 in 1000 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 10000 dilution for 1 hour at room temperature before imaging.
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![Western blot - Anti-KDM5C / Jarid1C / SMCX antibody [EPR23932-18] (ab259913)](https://www.abcam.com/ps/products/259/ab259913/Images/ab259913-457453-antiKDM5C--Jarid1C--SMCX-antibody-EPR2393218-western-blot-hap1.jpg)
Western blot - Anti-KDM5C / Jarid1C / SMCX antibody [EPR23932-18] (ab259913)All lanes : Anti-KDM5C / Jarid1C / SMCX antibody [EPR23932-18] (ab259913) at 1/1000 dilution
Lane 1 : HAP1 (human chronic myelogenous leukemia near-haploid cell), whole cell lysate
Lane 2 : HEK293T (human embryonic kidney epithelial cell), whole cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/50000 dilution (Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated)
Predicted band size: 175 kDa
Observed band size: 180 kDa why is the actual band size different from the predicted?Blocking and Diluting buffer and concentration: Intercept® (TBS) Blocking Buffer diluted with an equal volume of 0.1% TBS
Exposure time 70 seconds
Smaller bands are non-specific signal.
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![Western blot - Anti-KDM5C / Jarid1C / SMCX antibody [EPR23932-18] (ab259913)](https://www.abcam.com/ps/products/259/ab259913/Images/ab259913-457454-antiKDM5C--Jarid1C--SMCX-antibody-EPR2393218-western-blot-NCCIT.jpg)
Western blot - Anti-KDM5C / Jarid1C / SMCX antibody [EPR23932-18] (ab259913)Anti-KDM5C / Jarid1C / SMCX antibody [EPR23932-18] (ab259913) at 1/2000 dilution + NCCIT (human pluripotent embryonic carcinoma epithelial cell) whole cell lysate at 20 µg
Secondary
Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution (Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated)
Predicted band size: 175 kDa
Observed band size: 180 kDa why is the actual band size different from the predicted?Blocking and Diluting buffer and concentration: 5% NFDM/TBST
Exposure time 3 minutes
Smaller bands are non-specific signal.
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![Anti-KDM5C / Jarid1C / SMCX antibody [EPR23932-18] (ab259913)](https://www.abcam.com/ps/products/259/ab259913/Images/ab259913-1-Benefits-of-Recombinant-antibodies.jpg)
Anti-KDM5C / Jarid1C / SMCX antibody [EPR23932-18] (ab259913)
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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Datasheet download
Certificate of Compliance
References (0)
ab259913 has not yet been referenced specifically in any publications.
