Anti-KDM5C / Jarid1C / SMCX antibody (ab190180)
Key features and details
- Rabbit polyclonal to KDM5C / Jarid1C / SMCX
- Suitable for: ICC/IF, WB, IP
- Reacts with: Mouse, Rat, Chicken, Human
- Isotype: IgG
Overview
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Product name
Anti-KDM5C / Jarid1C / SMCX antibody
See all KDM5C / Jarid1C / SMCX primary antibodies -
Description
Rabbit polyclonal to KDM5C / Jarid1C / SMCX -
Host species
Rabbit -
Tested applications
Suitable for: ICC/IF, WB, IPmore details -
Species reactivity
Reacts with: Mouse, Rat, Chicken, Human -
Immunogen
Recombinant fragment corresponding to Human KDM5C/ Jarid1C/ SMCX aa 1000-1350. Isoform 4.
Database link: P41229-4 -
Positive control
- U2OS cells transfected with KDM5C / Jarid1C / SMCX siRNA, U2OS, mouse embryo fibroblasts (MEFs), rat INS1 and chicken DT40 cell lysates. U2OS cells.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.05% Sodium azide -
Concentration information loading...
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Purity
Whole antiserum -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab190180 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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ICC/IF |
1/2000.
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WB |
1/2000. Predicted molecular weight: 175 kDa.
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IP |
Use at an assay dependent concentration.
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Notes |
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ICC/IF
1/2000. |
WB
1/2000. Predicted molecular weight: 175 kDa. |
IP
Use at an assay dependent concentration. |
Target
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Function
Histone demethylase that specifically demethylates 'Lys-4' of histone H3, thereby playing a central role in histone code. Does not demethylate histone H3 'Lys-9', H3 'Lys-27', H3 'Lys-36', H3 'Lys-79' or H4 'Lys-20'. Demethylates trimethylated and dimethylated but not monomethylated H3 'Lys-4'. Participates in transcriptional repression of neuronal genes by recruiting histone deacetylases and REST at neuron-restrictive silencer elements. -
Tissue specificity
Expressed in all tissues examined. Highest levels found in brain and skeletal muscle. -
Involvement in disease
Defects in KDM5C are the cause of mental retardation syndromic X-linked JARID1C-related (MRXSJ) [MIM:300534]. MRXSJ is characterized by significantly sub-average general intellectual functioning associated with impairments in adaptative behavior and manifested during the developmental period. MRXSJ patients manifest mental retardation associated with variable features such as slowly progressive spastic paraplegia, seizures, facial dysmorphism. -
Sequence similarities
Belongs to the JARID1 histone demethylase family.
Contains 1 ARID domain.
Contains 1 JmjC domain.
Contains 1 JmjN domain.
Contains 2 PHD-type zinc fingers. -
Domain
The first PHD-type zinc finger domain recognizes and binds H3-K9Me3.
Both the JmjC domain and the JmjN domain are required for enzymatic activity. -
Cellular localization
Nucleus. - Information by UniProt
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Database links
- Entrez Gene: 8242 Human
- Entrez Gene: 20591 Mouse
- Entrez Gene: 317432 Rat
- Omim: 314690 Human
- SwissProt: P41229 Human
- SwissProt: P41230 Mouse
- Unigene: 631768 Human
- Unigene: 142655 Mouse
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Alternative names
- DXS1272E antibody
- Histone demethylase JARID1C antibody
- JARID1C antibody
see all
Images
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All lanes : Anti-KDM5C / Jarid1C / SMCX antibody (ab190180) at 1/2000 dilution
Lane 1 : Human U2OS cell lysate
Lane 2 : Human U2OS cells transfected with KDM5C / Jarid1C / SMCX siRNA
Lane 3 : Mouse embryo fibroblasts (MEFs)
Lane 4 : Rat INS1 cell lysate
Lane 5 : Chicken DT40 cell lysate
Lysates/proteins at 30 µg per lane.
Predicted band size: 175 kDa -
Western blot analysis with ab190180 of immunoprecipitated U2OS cells extracts:
Lane 1: Input.
Lane 2: Immunoprecipitation with control serum.
Lane 3: Immunoprecipitation with ab190180 (5 µL)
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Immunofluorescence analysis of U2OS cells labeling KDM5C / Jarid1C / SMCX using ab190180 at a 1/2000 dilution.
Protocols
Datasheets and documents
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Datasheet download
References (2)
ab190180 has been referenced in 2 publications.
- Gunn K et al. (R)-2-Hydroxyglutarate Inhibits KDM5 Histone Lysine Demethylases to Drive Transformation in IDH-Mutant Cancers. Cancer Discov 13:1478-1497 (2023). PubMed: 36847506
- Mu MD et al. Therapeutic effect of a histone demethylase inhibitor in Parkinson's disease. Cell Death Dis 11:927 (2020). WB ; Human . PubMed: 33116116