Key features and details
- Rabbit polyclonal to KDM5C / Jarid1C / SMCX
- Suitable for: WB, IP
- Reacts with: Human
- Isotype: IgG
Product nameAnti-KDM5C / Jarid1C / SMCX antibody
See all KDM5C / Jarid1C / SMCX primary antibodies
DescriptionRabbit polyclonal to KDM5C / Jarid1C / SMCX
Tested applicationsSuitable for: WB, IPmore details
Species reactivityReacts with: Human
Predicted to work with: Horse, Dog, Pig, Chimpanzee, Rhesus monkey, Gorilla, Orangutan, Elephant
Synthetic peptide corresponding to Human KDM5C/ Jarid1C/ SMCX aa 275-325.
Database link: P41229
- HeLa whole cell lysate.
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage bufferPreservative: 0.09% Sodium azide
Constituents: 0.1% BSA, Tris buffered saline
Concentration information loading...
PurityImmunogen affinity purified
- Epigenetics and Nuclear Signaling
- Nuclear Signaling Pathways
- Nuclear Receptors
Our Abpromise guarantee covers the use of ab72152 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/2000 - 1/10000. Detects a band of approximately 200 kDa (predicted molecular weight: 176 kDa).|
|IP||Use at 2 µg/mg of lysate.|
FunctionHistone demethylase that specifically demethylates 'Lys-4' of histone H3, thereby playing a central role in histone code. Does not demethylate histone H3 'Lys-9', H3 'Lys-27', H3 'Lys-36', H3 'Lys-79' or H4 'Lys-20'. Demethylates trimethylated and dimethylated but not monomethylated H3 'Lys-4'. Participates in transcriptional repression of neuronal genes by recruiting histone deacetylases and REST at neuron-restrictive silencer elements.
Tissue specificityExpressed in all tissues examined. Highest levels found in brain and skeletal muscle.
Involvement in diseaseDefects in KDM5C are the cause of mental retardation syndromic X-linked JARID1C-related (MRXSJ) [MIM:300534]. MRXSJ is characterized by significantly sub-average general intellectual functioning associated with impairments in adaptative behavior and manifested during the developmental period. MRXSJ patients manifest mental retardation associated with variable features such as slowly progressive spastic paraplegia, seizures, facial dysmorphism.
Sequence similaritiesBelongs to the JARID1 histone demethylase family.
Contains 1 ARID domain.
Contains 1 JmjC domain.
Contains 1 JmjN domain.
Contains 2 PHD-type zinc fingers.
DomainThe first PHD-type zinc finger domain recognizes and binds H3-K9Me3.
Both the JmjC domain and the JmjN domain are required for enzymatic activity.
- Information by UniProt
- DXS1272E antibody
- Histone demethylase JARID1C antibody
- JARID1C antibody
All lanes : Anti-KDM5C / Jarid1C / SMCX antibody (ab72152) at 0.04 µg/ml
Lane 1 : HeLa whole cell lysate at 50 µg
Lane 2 : HeLa whole cell lysate at 15 µg
Lane 3 : HeLa whole cell lysate at 5 µg
Predicted band size: 176 kDa
Observed band size: 200 kDa why is the actual band size different from the predicted?
Additional bands at: 220 kDa, 300 kDa. We are unsure as to the identity of these extra bands.
Detection of Human KDM5C / Jarid1C / SMCX by Immunoprecipitation in Whole cell lysate from HeLa cells (1 mg/IP, 20% of IP loaded) using ab72152 at 3 µg/mg lysate for IP and at 0.1 µg/ml for subsequent WB detection. Note: bands between 55 and 71 kDa represent the IgG chains.
ab72152 has been referenced in 1 publication.
- Liang Z et al. Proliferating cell nuclear antigen is required for loading of the SMCX/KMD5C histone demethylase onto chromatin. Epigenetics Chromatin 4:18 (2011). PubMed: 21996408