Anti-KDM5C / Jarid1C / SMCX antibody - N-terminal (ab155681)
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Overview
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Product nameAnti-KDM5C / Jarid1C / SMCX antibody - N-terminal
See all KDM5C / Jarid1C / SMCX primary antibodies -
DescriptionRabbit polyclonal to KDM5C / Jarid1C / SMCX - N-terminal
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Host speciesRabbit
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Tested applicationsSuitable for: WBmore details
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Species reactivityReacts with: Human
Predicted to work with: Mouse, Cow, Dog
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Immunogen
Recombinant fragment corresponding to Human KDM5C/ Jarid1C/ SMCX aa 10-291 (N terminal).
Database link: P41229 -
Positive control
- 293T, A431, H1299, HeLa, HepG2, MOLT4 and Raji whole cell lysates.
Properties
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FormLiquid
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Storage instructionsShipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
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Storage bufferpH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 0.75% Glycine, 1.21% Tris, 10% Glycerol -
Concentration information loading... -
PurityImmunogen affinity purified
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ClonalityPolyclonal
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IsotypeIgG
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Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
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Related Products
Applications
Our Abpromise guarantee covers the use of ab155681 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | 1/500 - 1/3000. Predicted molecular weight: 175 kDa. |
Target
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FunctionHistone demethylase that specifically demethylates 'Lys-4' of histone H3, thereby playing a central role in histone code. Does not demethylate histone H3 'Lys-9', H3 'Lys-27', H3 'Lys-36', H3 'Lys-79' or H4 'Lys-20'. Demethylates trimethylated and dimethylated but not monomethylated H3 'Lys-4'. Participates in transcriptional repression of neuronal genes by recruiting histone deacetylases and REST at neuron-restrictive silencer elements.
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Tissue specificityExpressed in all tissues examined. Highest levels found in brain and skeletal muscle.
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Involvement in diseaseDefects in KDM5C are the cause of mental retardation syndromic X-linked JARID1C-related (MRXSJ) [MIM:300534]. MRXSJ is characterized by significantly sub-average general intellectual functioning associated with impairments in adaptative behavior and manifested during the developmental period. MRXSJ patients manifest mental retardation associated with variable features such as slowly progressive spastic paraplegia, seizures, facial dysmorphism.
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Sequence similaritiesBelongs to the JARID1 histone demethylase family.
Contains 1 ARID domain.
Contains 1 JmjC domain.
Contains 1 JmjN domain.
Contains 2 PHD-type zinc fingers. -
DomainThe first PHD-type zinc finger domain recognizes and binds H3-K9Me3.
Both the JmjC domain and the JmjN domain are required for enzymatic activity. -
Cellular localizationNucleus.
- Information by UniProt
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Database links
- Entrez Gene: 281490 Cow
- Entrez Gene: 491894 Dog
- Entrez Gene: 8242 Human
- Entrez Gene: 20591 Mouse
- Omim: 314690 Human
- SwissProt: Q38JA7 Dog
- SwissProt: P41229 Human
- SwissProt: P41230 Mouse
see all -
Alternative names
- DXS1272E antibody
- Histone demethylase JARID1C antibody
- JARID1C antibody
see all
Images
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Western blot - Anti-KDM5C / Jarid1C / SMCX antibody - N-terminal (ab155681)All lanes : Anti-KDM5C / Jarid1C / SMCX antibody - N-terminal (ab155681) at 1/1000 dilution
Lane 1 : A431 whole cell lysate
Lane 2 : HeLa whole cell lysate
Lysates/proteins at 30 µg per lane.
Predicted band size: 175 kDa
5% SDS PAGE
Protocols
References
ab155681 has not yet been referenced specifically in any publications.
