Question (24434) | KDM5/JARID Activity Quantification Assay Kit (Fluorometric) (ab113464)

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Question

Phone call: Customer kindly asked about the target the antibody specific to?

Answer

Thank you for contacting us. Tri-methylated histone H3-K4 is a substrate for JARID enzyme which removes methyl group from substrate, which is then detected by antibodies present in the kit. So the kit antibodies will detect the JARID de-methylated products. The amount of demethylated products is proportional to the enzyme activity of JARID which can be fluorometrically measured by reading the fluorescence in a fluorescent microplate reader at 530 excitation and 590 emissions. The whole procedure is explained in the protocol section of this kit; which can be downloaded by clicking the following link; https://www.abcam.com/ps/products/113/ab113464/documents/ab113464%20EpiSeeker%20KDM5%20JARID%20Activity%20Quantification%20Assay%20Kit-Fluorometric%20(Website).pdf I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

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