Question (63114) | KDM5/JARID Activity Quantification Assay Kit (Fluorometric) (ab113464)

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Question

We would like to quantify H3K4me3 demethylation activity in mouse embryonic stem cell extracts. You have two kits listed that seem to both serve that purpose: 113464 and 113455. - What is the difference between these two? - Do you recommend one over the other for K4me3 demethylation? - Has any of them been used in any publications?

Answer

Thank you for your interest in ab113455 & ab113464.

You are correct that both these kits measure demethylase activity. The major difference between these 2 kits is the substrate captured on the well. For ab113455, the substrate is a dimethylated histone H3-K4 substrate while the substrate used in ab113464 is a trimethylated histone H3-K4 substrate.

Due to these different substrates, ab113455 will detect LSD1 type demethylase activity which can remove mono and dimethyl groups while ab113464 will detect JARID type demethylase activity which removes trimethyl groups from H3 K4.

So it seems ab113464 would be the proper choice for your interest in studying H3K4me3 demethylation activity.

As far as we know neither of these kits have been used in published works.

I hope this is helpful. Please contact us again if you have any further questions.

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