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  1. Link

    kdm6b-jmjd3-antibody-ab169197.pdf

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Epigenetics and Nuclear Signaling Transcription Domain Families Developmental Families HOX
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Anti-KDM6B / JMJD3 antibody (ab169197)

  • Datasheet
  • SDS
Reviews (1) Submit a question References (4)

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  • Immunocytochemistry/ Immunofluorescence - Anti-KDM6B / JMJD3 antibody (ab169197)
  • Western blot - Anti-KDM6B / JMJD3 antibody (ab169197)

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Primary

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Secondary

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Protein

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  • Datasheet
  • References (4)
  • Protocols

Overview

  • Product name

    Anti-KDM6B / JMJD3 antibody
    See all KDM6B / JMJD3 primary antibodies
  • Description

    Rabbit polyclonal to KDM6B / JMJD3
  • Host species

    Rabbit
  • Tested applications

    Suitable for: ICC/IF, WBmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse, Rat, Rabbit, Horse, Cow, Chimpanzee, Macaque monkey, Gorilla
  • Immunogen

    Synthetic peptide corresponding to Mouse KDM6B/ JMJD3 aa 50-150 conjugated to keyhole limpet haemocyanin.
    Database link: Q5NCY0

  • Positive control

    • This antibody gave a positive signal in both HeLa whole cell and HeLa Nuclear lysates. This antibody gave a positive result when used in the following methanol fixed cell lines: MCF-7

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.40
    Preservative: 0.02% Sodium azide
    Constituent: PBS

    Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help.
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

    • Epigenetics and Nuclear Signaling
    • Transcription
    • Domain Families
    • Developmental Families
    • HOX
    • Epigenetics and Nuclear Signaling
    • Chromatin Modifying Enzymes
    • Jumonji containing proteins

Associated products

  • Compatible Secondaries

    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
    • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
  • Isotype control

    • Rabbit IgG, polyclonal - Isotype Control (ChIP Grade) (ab171870)
  • Recombinant Protein

    • Recombinant human KDM6B / JMJD3 protein (ab196420)

Applications

Our Abpromise guarantee covers the use of ab169197 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use a concentration of 5 µg/ml.
WB Use a concentration of 1 µg/ml. Detects a band of approximately 177 kDa (predicted molecular weight: 177 kDa).

Target

  • Function

    Histone demethylase that specifically demethylates 'Lys-27' of histone H3, thereby playing a central role in histone code. Demethylates trimethylated and dimethylated H3 'Lys-27'. Plays a central role in regulation of posterior development, by regulating HOX gene expression. Involved in inflammatory response by participating in macrophage differentiation in case of inflammation by regulating gene expression and macrophage differentiation.
  • Sequence similarities

    Belongs to the UTX family.
    Contains 1 JmjC domain.
  • Cellular localization

    Nucleus.
  • Target information above from: UniProt accession O15054 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 23135 Human
    • Entrez Gene: 216850 Mouse
    • Entrez Gene: 363630 Rat
    • Omim: 611577 Human
    • SwissProt: O15054 Human
    • SwissProt: Q5NCY0 Mouse
    • Unigene: 223678 Human
    • Unigene: 261201 Mouse
    • Unigene: 472522 Mouse
    see all
  • Alternative names

    • Histone demethylase JMJD3 antibody
    • JmjC domain containing protein 3 antibody
    • JmjC domain-containing protein 3 antibody
    • Jumonji D3 antibody
    • Jumonji domain containing 3 antibody
    • Jumonji domain containing 3 histone lysine demethylase antibody
    • Jumonji domain containing protein 3 antibody
    • Jumonji domain-containing protein 3 antibody
    • Kdm6b antibody
    • KDM6B_HUMAN antibody
    • KIAA0346 antibody
    • Lysine demethylase 6B antibody
    • Lysine K specific demethylase 6B antibody
    • Lysine specific demethylase 6B antibody
    • Lysine-specific demethylase 6B antibody
    see all

Images

  • Immunocytochemistry/ Immunofluorescence - Anti-KDM6B / JMJD3 antibody (ab169197)
    Immunocytochemistry/ Immunofluorescence - Anti-KDM6B / JMJD3 antibody (ab169197)

    ab169197 staining KDM6B/JMJD3 in MCF7 cells. The cells were fixed with 100% methanol (5min), permeabilized with 0.1% Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1%PBS-Tween for 1h. The cells were then incubated overnight at +4°C with ab169197 at 1μg/ml and ab7291, Mouse monoclonal [DM1A] to alpha Tubulin - Loading Control, at 1/1000 dilution. Cells were then incubated with ab150120, Goat polyclonal Secondary Antibody to Mouse at 1/1000 dilution (shown in pseudocolour red) and ab150081, Goat polyclonal Secondary Antibody to Rabbit IgG at 1/1000 dilution (shown in green). Nuclear DNA was labelled with DAPI (shown in blue).

  • Western blot - Anti-KDM6B / JMJD3 antibody (ab169197)
    Western blot - Anti-KDM6B / JMJD3 antibody (ab169197)
    All lanes : Anti-KDM6B / JMJD3 antibody (ab169197) at 1 µg/ml

    Lane 1 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
    Lane 2 : HeLa (Human epithelial carcinoma cell line) Nuclear Lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/10000 dilution

    Developed using the ECL technique.

    Performed under reducing conditions.

    Predicted band size: 177 kDa
    Observed band size: 177 kDa


    Exposure time: 4 minutes


    Due to the cellular localisation of KDM6B / JMJD3 an increase in expression is expected within the Nuclear fraction.

    This blot was produced using a 3-8% Tris Acetate gel under the TA buffer system. The gel was run at 150V for 60 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 2% Bovine Serum Albumin before being incubated with ab169197 overnight at 4°C. Antibody binding was detected using an anti-rabbit antibody conjugated to HRP, and visualised using ECL development solution.

Protocols

  • Western blot protocols
  • Immunocytochemistry & immunofluorescence protocols

Datasheets and documents

    • Datasheet
    • SDS
  • References

    This product has been referenced in:

    • Guo Z  et al. JMJD3 inhibition protects against isoproterenol-induced cardiac hypertrophy by suppressing ß-MHC expression. Mol Cell Endocrinol N/A:N/A (2018). Read more (PubMed: 29753027) »
    • Daures M  et al. A new metabolic gene signature in prostate cancer regulated by JMJD3 and EZH2. Oncotarget 9:23413-23425 (2018). Read more (PubMed: 29805743) »
    See all 4 Publications for this product

    Publishing research using ab169197? Please let us know so that we can cite the reference in this datasheet.

    Customer reviews and Q&As

    Show All Reviews Q&A
    Submit a review Submit a question

    Western blot abreview for Anti-KDM6B / JMJD3 antibody

    Inconclusive
    Abreviews
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Rat Tissue lysate - whole (spinal cord)
    Gel Running Conditions
    Reduced Denaturing (8%)
    Loading amount
    70 µg
    Specification
    spinal cord
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C
    Read More

    Abcam user community

    Verified customer

    Submitted Sep 15 2015

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