Key features and details
- Rabbit polyclonal to KDS
- Suitable for: WB, IP
- Reacts with: Human
- Isotype: IgG
Product nameAnti-KDS antibody
See all KDS primary antibodies
DescriptionRabbit polyclonal to KDS
Tested applicationsSuitable for: WB, IPmore details
Species reactivityReacts with: Human
Predicted to work with: Mouse, Rat, Rabbit, Horse, Guinea pig, Cow, Dog, Pig, Xenopus laevis, Rhesus monkey, Gorilla, Chinese hamster, Orangutan, Elephant
Synthetic peptide corresponding to Human KDS (C terminal).
Database link: Q9H2K8
- HeLa whole cell lysates.
This product was previously labelled as TAOK3
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage bufferPreservative: 0.09% Sodium azide
Constituents: 1.815% Tris, 1.764% Sodium citrate, 0.021% PBS
Concentration information loading...
PurityImmunogen affinity purified
Purification notesab70297 was affinity purified using an epitope specific to JIK immobilized on solid support.
Our Abpromise guarantee covers the use of ab70297 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
WB: 1/5000 - 1/25000. Detects a band of approximately 120 kDa (predicted molecular weight: 105 kDa).
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
FunctionInhibits the basal activity of Jun kinase. Negatively regulated by epidermal growth factor (EGF). When overexpressed, may activate ERK1/ERK2 and JNK/SAPK.
Tissue specificityUbiquitously expressed at a low level, and highly expressed in peripheral blood leukocytes (PBLs), thymus, spleen, kidney, skeletal muscle, heart and liver.
Sequence similaritiesBelongs to the protein kinase superfamily. STE Ser/Thr protein kinase family. STE20 subfamily.
Contains 1 protein kinase domain.
modificationsAutophosphorylated. Phosphorylated upon DNA damage, probably by ATM or ATR.
Cellular localizationCytoplasm. Cell membrane. Also localized to the peripheral cell membrane.
- Information by UniProt
- CTCL tumor antigen HD CL 09 antibody
- CTCL-associated antigen HD-CL-09 antibody
- Cutaneous T cell lymphoma tumor antigen HD CL 09 antibody
All lanes : Anti-KDS antibody (ab70297) at 0.1 µg/ml
Lane 1 : HeLa whole cell lysate at 50 µg
Lane 2 : HeLa whole cell lysate at 15 µg
Developed using the ECL technique.
Predicted band size: 105 kDa
Observed band size: 120 kDa why is the actual band size different from the predicted?
Additional bands at: 45 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 3 minutes
Western Blot showing Lane 2: ab70297 staining JIK in HeLa whole cell lysates immunoprecipitated using ab70297 at 3µg/mg lysate (1mg/lane). Lane 1: control IgG used for immunoprecipitation.Detection: Chemiluminescence with an exposure time of 3 minutes
ab70297 has been referenced in 1 publication.
- Ormonde JVS et al. TAOK3 Regulates Canonical TCR Signaling by Preventing Early SHP-1-Mediated Inactivation of LCK. J Immunol 201:3431-3442 (2018). PubMed: 30373850