Recombinant Anti-KDS antibody [EPR4947(2)] (ab150388)
![Western blot - Anti-KDS antibody [EPR4947(2)] (ab150388)](https://www.abcam.com/ps/products/150/ab150388/Images/ab150388-450128-rabbit-monoclonal-epr49472-to-kds-western-blot-hela-jurkat-daudi-lysates.jpg "Western blot - Anti-KDS antibody [EPR4947(2)] (ab150388)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR4947(2)] to KDS
- Suitable for: WB
- Knockout validated
- Reacts with: Human
Related conjugates and formulations
Overview
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Product name
Anti-KDS antibody [EPR4947(2)]
See all KDS primary antibodies -
Description
Rabbit monoclonal [EPR4947(2)] to KDS -
Host species
Rabbit -
Tested applications
Suitable for: WBmore details
Unsuitable for: Flow Cyt,ICC/IF or IP -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat
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Immunogen
Synthetic peptide within Human KDS aa 850 to the C-terminus (C terminal). The exact sequence is proprietary.
Database link: Q9H2K8 -
Positive control
- Jurkat, 293T, K562 and HeLa cell lysates
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General notes
This antibody was developed as part of a collaboration between Epitomics and the lab of Melanie Cobb at UT Southwestern Medical Center.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR4947(2) -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Isotype control
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KO cell lines
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KO cell lysates
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Positive Controls
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab150388 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB |
1/1000 - 1/10000. Predicted molecular weight: 105 kDa.
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| Notes |
|---|
|
WB
1/1000 - 1/10000. Predicted molecular weight: 105 kDa. |
Target
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Function
Inhibits the basal activity of Jun kinase. Negatively regulated by epidermal growth factor (EGF). When overexpressed, may activate ERK1/ERK2 and JNK/SAPK. -
Tissue specificity
Ubiquitously expressed at a low level, and highly expressed in peripheral blood leukocytes (PBLs), thymus, spleen, kidney, skeletal muscle, heart and liver. -
Sequence similarities
Belongs to the protein kinase superfamily. STE Ser/Thr protein kinase family. STE20 subfamily.
Contains 1 protein kinase domain. -
Post-translational
modificationsAutophosphorylated. Phosphorylated upon DNA damage, probably by ATM or ATR. -
Cellular localization
Cytoplasm. Cell membrane. Also localized to the peripheral cell membrane. - Information by UniProt
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Database links
- Entrez Gene: 51347 Human
- Entrez Gene: 330177 Mouse
- Entrez Gene: 304530 Rat
- SwissProt: Q9H2K8 Human
- SwissProt: Q9HC79 Human
- SwissProt: Q8BYC6 Mouse
- SwissProt: Q53UA7 Rat
- Unigene: 644420 Human
see all -
Alternative names
- CTCL tumor antigen HD CL 09 antibody
- CTCL-associated antigen HD-CL-09 antibody
- Cutaneous T cell lymphoma tumor antigen HD CL 09 antibody
see all
Images
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Western blot - Anti-KDS antibody [EPR4947(2)] (ab150388)All lanes : Anti-KDS antibody [EPR4947(2)] (ab150388) at 1/1000 dilution
Lane 1 : Wild-type HeLa cell lysate at 40 µg
Lane 2 : TAOK3 knockout HeLa cell lysate at 40 µg
Lane 3 : Jurkat cell lysate at 10 µg
Lane 4 : Daudi cell lysate at 10 µg
Performed under reducing conditions.
Predicted band size: 105 kDa
Observed band size: 105 kDaLanes 1- 4: Merged signal (red and green). Green - ab150388 observed at 105 kDa. Red - Anti-GAPDH antibody [6C5] - Loading Control (ab8245) observed at 37 kDa.
ab150388 was shown to react with KDS in wild-type HeLa cells in western blot. Loss of signal was observed when knockout cell line ab265108 (knockout cell lysate ab258222) was used. Wild-type HeLa and TAOK3 knockout HeLa cell lysates were subjected to SDS-PAGE. Membrane was blocked for 1 hour at room temperature in 0.1% TBST with 3% non-fat dried milk. ab150388 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) overnight at 4°C at a 1 in 1000 dilution and a 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye®800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye®680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.
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![Western blot - Anti-KDS antibody [EPR4947(2)] (ab150388)](https://www.abcam.com/ps/products/150/ab150388/Images/ab150388-156990-anti-taok3-antibody-epr49472-western-blot.jpg)
Western blot - Anti-KDS antibody [EPR4947(2)] (ab150388)All lanes : Anti-KDS antibody [EPR4947(2)] (ab150388) at 1/1000 dilution
Lane 1 : Jurkat cell lysate
Lane 2 : 293T cell lysate
Lane 3 : K562 cell lysate
Lane 4 : HeLa cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : HRP labelled goat anti-rabbit antibody at 1/2000 dilution
Predicted band size: 105 kDa -
![Anti-KDS antibody [EPR4947(2)] (ab150388)](https://www.abcam.com/ps/products/150/ab150388/Images/ab150388-2-benefits-of-recombinant-antibodies.png)
Anti-KDS antibody [EPR4947(2)] (ab150388)
Datasheets and documents
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SDS download
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Datasheet download
References (1)
ab150388 has been referenced in 1 publication.
- Ormonde JVS et al. TAOK3 Regulates Canonical TCR Signaling by Preventing Early SHP-1-Mediated Inactivation of LCK. J Immunol 201:3431-3442 (2018). PubMed: 30373850