Recombinant Anti-KDS antibody [EPR4947(2)] - BSA and Azide free (ab248958)
![Western blot - Anti-KDS antibody [EPR4947(2)] - BSA and Azide free (ab248958)](https://www.abcam.com/ps/products/248/ab248958/Images/ab248958-450140-rabbit-monoclonal-epr49472-to-kds-western-blot-hela-jurkat-daudi-lysates.jpg "Western blot - Anti-KDS antibody [EPR4947(2)] - BSA and Azide free (ab248958)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR4947(2)] to KDS - BSA and Azide free
- Suitable for: WB
- Knockout validated
- Reacts with: Human
Related conjugates and formulations
Overview
-
Product name
Anti-KDS antibody [EPR4947(2)] - BSA and Azide free
See all KDS primary antibodies -
Description
Rabbit monoclonal [EPR4947(2)] to KDS - BSA and Azide free -
Host species
Rabbit -
Tested applications
Suitable for: WBmore details
Unsuitable for: Flow Cyt,ICC or IP -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat
-
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
-
Positive control
- WB: HeLa, Jurkat, Daudi, HEK293T and K562 cell lysates.
-
General notes
ab248958 is the carrier-free version of ab150388.
This antibody was developed as part of a collaboration between Epitomics and the lab of Melanie Cobb at UT Southwestern Medical Center.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
Storage buffer
pH: 7.2
Constituent: PBS -
Carrier free
Yes -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR4947(2) -
Isotype
IgG -
Research areas
Associated products
-
Alternative Versions
-
Conjugation kits
-
Isotype control
-
KO cell lines
-
KO cell lysates
-
Positive Controls
-
Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab248958 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB |
Use at an assay dependent concentration. Predicted molecular weight: 105 kDa.
|
| Notes |
|---|
|
WB
Use at an assay dependent concentration. Predicted molecular weight: 105 kDa. |
Target
-
Function
Inhibits the basal activity of Jun kinase. Negatively regulated by epidermal growth factor (EGF). When overexpressed, may activate ERK1/ERK2 and JNK/SAPK. -
Tissue specificity
Ubiquitously expressed at a low level, and highly expressed in peripheral blood leukocytes (PBLs), thymus, spleen, kidney, skeletal muscle, heart and liver. -
Sequence similarities
Belongs to the protein kinase superfamily. STE Ser/Thr protein kinase family. STE20 subfamily.
Contains 1 protein kinase domain. -
Post-translational
modificationsAutophosphorylated. Phosphorylated upon DNA damage, probably by ATM or ATR. -
Cellular localization
Cytoplasm. Cell membrane. Also localized to the peripheral cell membrane. - Information by UniProt
-
Database links
- Entrez Gene: 51347 Human
- Entrez Gene: 330177 Mouse
- Entrez Gene: 304530 Rat
- SwissProt: Q9H2K8 Human
- SwissProt: Q9HC79 Human
- SwissProt: Q8BYC6 Mouse
- SwissProt: Q53UA7 Rat
- Unigene: 644420 Human
see all -
Alternative names
- CTCL tumor antigen HD CL 09 antibody
- CTCL-associated antigen HD-CL-09 antibody
- Cutaneous T cell lymphoma tumor antigen HD CL 09 antibody
see all
Images
-
Western blot - Anti-KDS antibody [EPR4947(2)] - BSA and Azide free (ab248958)All lanes : Anti-KDS antibody [EPR4947(2)] (ab150388) at 1/1000 dilution
Lane 1 : Wild-type HeLa cell lysate at 40 µg
Lane 2 : TAOK3 knockout HeLa cell lysate at 40 µg
Lane 3 : Jurkat cell lysate at 10 µg
Lane 4 : Daudi cell lysate at 10 µg
Performed under reducing conditions.
Predicted band size: 105 kDa
Observed band size: 105 kDaThis data was developed using the same antibody clone in a different buffer formulation (ab150388).
Lanes 1- 4: Merged signal (red and green). Green - ab150388 observed at 105 kDa. Red - Anti-GAPDH antibody [6C5] - Loading Control (ab8245) observed at 37 kDa.
ab150388 was shown to react with KDS in wild-type HeLa cells in western blot. Loss of signal was observed when knockout cell line ab265108 (knockout cell lysate ab258222) was used. Wild-type HeLa and TAOK3 knockout HeLa cell lysates were subjected to SDS-PAGE. Membrane was blocked for 1 hour at room temperature in 0.1% TBST with 3% non-fat dried milk. ab150388 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) overnight at 4°C at a 1 in 1000 dilution and a 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye®800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye®680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.
-
![Western blot - Anti-KDS antibody [EPR4947(2)] - BSA and Azide free (ab248958)](https://www.abcam.com/ps/products/248/ab248958/Images/ab248958-398487-anti-kds-antibody-epr4947-2-bsa-and-azide-free-western-blot.jpg)
Western blot - Anti-KDS antibody [EPR4947(2)] - BSA and Azide free (ab248958)All lanes : Anti-KDS antibody [EPR4947(2)] (ab150388) at 1/1000 dilution
Lane 1 : Jurkat cell lysate
Lane 2 : 293T cell lysate
Lane 3 : K562 cell lysate
Lane 4 : HeLa cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : HRP labelled goat anti-rabbit antibody at 1/2000 dilution
Predicted band size: 105 kDaThis data was developed using ab150388, the same antibody clone in a different buffer formulation.
-
![Anti-KDS antibody [EPR4947(2)] - BSA and Azide free (ab248958)](https://www.abcam.com/ps/products/248/ab248958/Images/ab248958-2-benefits-of-recombinant-antibodies.png)
Anti-KDS antibody [EPR4947(2)] - BSA and Azide free (ab248958)
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
-
Datasheet download
Certificate of Compliance
References (0)
ab248958 has not yet been referenced specifically in any publications.
