Product nameKDS overexpression 293T lysate (whole cell)
DescriptionKDS overexpression 293T lysate (whole cell)
ab94147 is a 293T cell transfected lysate in which Human JIK has been transiently over-expressed using a pCMV-JIK. The lysate is provided in 1X Sample Buffer. Note: For more details about how the transfected lysate was prepared view preparation notes.
Tested applicationsSuitable for: WBmore details
Storage instructionsShipped on dry ice. Upon delivery aliquot and store at -20ºC. Avoid freeze / thaw cycles.
Storage bufferConstituents: 0.01% Bromophenol blue, 2.3% Beta mercaptoethanol, 2% Sodium lauryl sulfate, 0.788% Tris HCl, 10% Glycerol (glycerin, glycerine)
Concentration information loading...
BackgroundFunction: Inhibits the basal activity of Jun kinase. Negatively regulated by epidermal growth factor (EGF). When overexpressed, may activate ERK1/ERK2 and JNK/SAPK. Tissue specificity: Ubiquitously expressed at a low level, and highly expressed in peripheral blood leukocytes (PBLs), thymus, spleen, kidney, skeletal muscle, heart and liver. Similarity: Belongs to the protein kinase superfamily. STE Ser/Thr protein kinase family. STE20 subfamily. Contains 1 protein kinase domain. PTM: Autophosphorylated. Phosphorylated upon DNA damage, probably by ATM or ATR.
Our Abpromise guarantee covers the use of ab94147 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use at an assay dependent dilution.|
ab94147 at 15µg/lane on an SDS-PAGE gel.
All lanes : Anti-KDS antibody (ab57359) at 1/500 dilution
Lane 1 :
KDS overexpression 293T lysate (whole cell) (ab94147)
Lane 2 : 293T non-transfected lysate
Lysates/proteins at 25 µg per lane.
All lanes : Goat Anti-mouse IgG (H and L) HRP conjugated at 1/2500 dilution
ab94147 has not yet been referenced specifically in any publications.