Overview

  • Product name

    Anti-Keap1 antibody [1B4]
    See all Keap1 primary antibodies
  • Description

    Mouse monoclonal [1B4] to Keap1
  • Host species

    Mouse
  • Tested applications

    Suitable for: WB, IHC-P, Flow Cytmore details
  • Species reactivity

    Reacts with: Mouse, Human
  • Immunogen

    Recombinant full length Human Keap1 produced in HEK293T cells (NP_987096).

  • Positive control

    • HEK293T cell lysate transfected with pCMV6-ENTRY Keap1 cDNA; HEK293T cells transfected with pCMV6-ENTRY Keap1 overexpress plasmid; Jurkat and HeLa cells; Human endometrium, endometrium adenocarcinoma and bladder carcinoma tissues.
  • General notes

    Dilute in PBS (pH7.3) before use. Stable for 12 months from date of receipt.

    This product was changed from ascites to tissue culture supernatant on 6th September 2018. Please note that the dilutions may need to be adjusted accordingly. If you have any questions, please do not hesitate to contact our scientific support team.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
  • Storage buffer

    pH: 7.30
    Preservative: 0.02% Sodium azide
    Constituents: 48% PBS, 1% BSA, 50% Glycerol
  • Concentration information loading...
  • Purity

    Protein G purified
  • Purification notes

    ab119403 was purified from TCS by affinity chromatography.
  • Clonality

    Monoclonal
  • Clone number

    1B4
  • Isotype

    IgG1
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab119403 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/2000. Predicted molecular weight: 70 kDa.
IHC-P 1/150.
Flow Cyt 1/100.

ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.

 

Target

  • Function

    Retains NFE2L2/NRF2 in the cytosol. Functions as substrate adapter protein for the E3 ubiquitin ligase complex formed by CUL3 and RBX1. Targets NFE2L2/NRF2 for ubiquitination and degradation by the proteasome, thus resulting in the suppression of its transcriptional activity and the repression of antioxidant response element-mediated detoxifying enzyme gene expression. May also retain BPTF in the cytosol. Targets PGAM5 for ubiquitination and degradation by the proteasome.
  • Tissue specificity

    Broadly expressed, with highest levels in skeletal muscle.
  • Sequence similarities

    Contains 1 BACK (BTB/Kelch associated) domain.
    Contains 1 BTB (POZ) domain.
    Contains 6 Kelch repeats.
  • Domain

    The Kelch repeats mediate interaction with NF2L2/NRF2, BPTF and PGAM5.
  • Post-translational
    modifications

    Ubiquitinated and subject to proteasomal degradation.
  • Cellular localization

    Cytoplasm. Nucleus. Shuttles between cytoplasm and nucleus.
  • Information by UniProt
  • Database links

  • Alternative names

    • Cytosolic inhibitor of Nrf2 antibody
    • INrf 2 antibody
    • INrf2 antibody
    • Keap 1 antibody
    • KEAP1 antibody
    • KEAP1_HUMAN antibody
    • Kelch like ECH associated protein 1 antibody
    • Kelch like family member 19 antibody
    • Kelch like protein 19 antibody
    • Kelch-like ECH-associated protein 1 antibody
    • Kelch-like protein 19 antibody
    • KIAA0132 antibody
    • KLHL 19 antibody
    • KLHL19 antibody
    • MGC10630 antibody
    • MGC1114 antibody
    • MGC20887 antibody
    • MGC4407 antibody
    • MGC9454 antibody
    see all

Images

  • All lanes : Anti-Keap1 antibody [1B4] (ab119403) at 1/500 dilution

    Lane 1 : HEK293T cell lysate transfected with pCMV6-ENTRY control cDNA
    Lane 2 : HEK293T cell lysate transfected with pCMV6-ENTRY Keap1 cDNA

    Lysates/proteins at 5 µg per lane.

    Developed using the ECL technique.

    Predicted band size: 70 kDa



    HEK293T cell lysates were generated from transient transfection of the cDNA clone (RC202189)
  • All lanes : Anti-Keap1 antibody [1B4] (ab119403) at 1/500 dilution

    Lane 1 : HepG2 cell extract
    Lane 2 : HeLa cell extract
    Lane 3 : SVT2 cell extract
    Lane 4 : A549 cell extract
    Lane 5 : COS7 cell extract
    Lane 6 : Jurkat cell extract
    Lane 7 : MDCK cell extract
    Lane 8 : PC12 cell extract
    Lane 9 : MCF7 cell extract

    Lysates/proteins at 35 µg per lane.

    Developed using the ECL technique.

    Predicted band size: 70 kDa



    HEK293T cell lysates were generated from transient transfection of the cDNA clone (RC202189)
  • ab119403 at 1/150 dilution staining Keap1 in paraffin-embedded Human endometrium tissue by Immunohistochemistry.
  • ab119403 at 1/150 dilution staining Keap1 in paraffin-embedded Human endometrium adenocarcinoma tissue by Immunohistochemistry.
  • ab119403 at 1/150 dilution staining Keap1 in paraffin-embedded Human bladder carcinoma tissue by Immunohistochemistry.
  • ab119403 at 1/100 dilution staining Keap1 in HEK293T cells transfected with either pCMV6-ENTRY Keap1 overexpress plasmid (Red) or empty vector control plasmid (Blue) and then analysed by Flow Cytometry.
  • ab119403 at 1/100 dilution staining Keap1 in HeLa cells by Flow cytometry (Red) compared to a nonspecific negative control antibody (Blue).
  • ab119403 at 1/100 dilution staining Keap1 in Jurkat cells by Flow cytometry (Red) compared to a nonspecific negative control antibody (Blue).

References

This product has been referenced in:

  • Huang R  et al. MiR-34b Protects Against Focal Cerebral Ischemia-Reperfusion (I/R) Injury in Rat by Targeting Keap1. J Stroke Cerebrovasc Dis 28:1-9 (2019). Read more (PubMed: 30539753) »
  • Huang H  et al. Downregulation of Keap1 contributes to poor prognosis and Axitinib resistance of renal cell carcinoma via upregulation of Nrf2 expression. Int J Mol Med 43:2044-2054 (2019). Read more (PubMed: 30896860) »
See all 5 Publications for this product

Customer reviews and Q&As

1-3 of 3 Abreviews or Q&A

Application
Western blot
Sample
Human Cell lysate - whole cell (C42B Prostate cancer cell line)
Gel Running Conditions
Reduced Denaturing
Loading amount
20 µg
Specification
C42B Prostate cancer cell line
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C

Dr. Dimitra Kalamida

Verified customer

Submitted May 14 2019

Application
Immunocytochemistry/ Immunofluorescence
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Sample
Human Cell (prostate)
Specification
prostate
Permeabilization
Yes - triton
Fixative
Formaldehyde

Abcam user community

Verified customer

Submitted Jul 28 2014

Answer

Thank you for taking the time to contact us. I am sorry to hear you have had difficulty obtaining satisfactory results from this antibody.

I would like to reassure you that this antibody is tested and covered by our 6 month guarantee for IHC-P and inhuman and mouse. In the event that a product is not functioning in the tested applications and species cited on the product data sheet (and the problem has been reported within 6 months of purchase), we will be pleased to provide a credit note, free of charge replacement or refund.

I would like to investigate this particular case further for you, and also obtain more information for our quality monitoring records. In order to proceed with this, I have enclosed a technical questionnaire below. I would appreciate if you could complete this. It will help you put the information we require together very easily.

I would appreciate if you could also provide an image which would help us to assess the results.

Thank you for your time and cooperation. We look forward to receiving the completed questionaire.


Order Details
Antibody code:

Lot number:

Purchase order number
or preferably Abcam order number:


General Information
Antibody storage conditions (temperature/reconstitution etc)


Description of the problem (high background, low signal, non-specific satining etc.)

Sample (Species/Tissue/Cell Type/Cell Line etc.)


Fixation of sample (Ethanol/Methanol/Acetone/Paraformaldehyde/Other/Duration etc.)


Antigen retrieval (Enzymatic method, Heat mediated technique etc.)


Permeabilization step


Blocking conditions (Buffer/time period, Blocking agent etc.)


Primary Antibody (Manufacturer/Species/Diluent/Dilution/Incubation time, Wash step)


Secondary Antibody (Manufacturer/Species/Diluent/Dilution/Incubation time, Wash step)


Detection method


Positive and negative controls used (please specify)


Optimization attempts (problem solving)
How many times have you tried the IHC?



Have you run a "No Primary" control?
Yes No

Do you obtain the same results every time?
Yes No


What steps have you altered?


Additional Notes


We would appreciate if you are also able to provide and image which woudl help us to assess the results

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