Overview

  • Product name
    Anti-Keap1 antibody [1B4]
    See all Keap1 primary antibodies
  • Description
    Mouse monoclonal [1B4] to Keap1
  • Host species
    Mouse
  • Tested applications
    Suitable for: WB, IHC-P, Flow Cytmore details
  • Species reactivity
    Reacts with: Mouse, Human
  • Immunogen

    Recombinant full length Human Keap1 produced in HEK293T cells (NP_987096).

  • Positive control
    • HEK293T cell lysate transfected with pCMV6-ENTRY Keap1 cDNA; HEK293T cells transfected with pCMV6-ENTRY Keap1 overexpress plasmid; Jurkat and HeLa cells; Human endometrium, endometrium adenocarcinoma and bladder carcinoma tissues.
  • General notes

    Dilute in PBS (pH7.3) before use. Stable for 12 months from date of receipt.

    This product was changed from ascites to tissue culture supernatant on 6th September 2018. Please note that the dilutions may need to be adjusted accordingly. If you have any questions, please do not hesitate to contact our scientific support team.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
  • Storage buffer
    pH: 7.30
    Preservative: 0.02% Sodium azide
    Constituents: 48% PBS, 1% BSA, 50% Glycerol
  • Concentration information loading...
  • Purity
    Protein G purified
  • Purification notes
    ab119403 was purified from TCS by affinity chromatography.
  • Clonality
    Monoclonal
  • Clone number
    1B4
  • Isotype
    IgG1
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab119403 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/2000. Predicted molecular weight: 70 kDa.
IHC-P 1/150.
Flow Cyt 1/100.

ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.

 

Target

  • Function
    Retains NFE2L2/NRF2 in the cytosol. Functions as substrate adapter protein for the E3 ubiquitin ligase complex formed by CUL3 and RBX1. Targets NFE2L2/NRF2 for ubiquitination and degradation by the proteasome, thus resulting in the suppression of its transcriptional activity and the repression of antioxidant response element-mediated detoxifying enzyme gene expression. May also retain BPTF in the cytosol. Targets PGAM5 for ubiquitination and degradation by the proteasome.
  • Tissue specificity
    Broadly expressed, with highest levels in skeletal muscle.
  • Sequence similarities
    Contains 1 BACK (BTB/Kelch associated) domain.
    Contains 1 BTB (POZ) domain.
    Contains 6 Kelch repeats.
  • Domain
    The Kelch repeats mediate interaction with NF2L2/NRF2, BPTF and PGAM5.
  • Post-translational
    modifications
    Ubiquitinated and subject to proteasomal degradation.
  • Cellular localization
    Cytoplasm. Nucleus. Shuttles between cytoplasm and nucleus.
  • Information by UniProt
  • Database links
  • Alternative names
    • Cytosolic inhibitor of Nrf2 antibody
    • INrf 2 antibody
    • INrf2 antibody
    • Keap 1 antibody
    • KEAP1 antibody
    • KEAP1_HUMAN antibody
    • Kelch like ECH associated protein 1 antibody
    • Kelch like family member 19 antibody
    • Kelch like protein 19 antibody
    • Kelch-like ECH-associated protein 1 antibody
    • Kelch-like protein 19 antibody
    • KIAA0132 antibody
    • KLHL 19 antibody
    • KLHL19 antibody
    • MGC10630 antibody
    • MGC1114 antibody
    • MGC20887 antibody
    • MGC4407 antibody
    • MGC9454 antibody
    see all

Images

  • All lanes : Anti-Keap1 antibody [1B4] (ab119403) at 1/500 dilution

    Lane 1 : HEK293T cell lysate transfected with pCMV6-ENTRY control cDNA
    Lane 2 : HEK293T cell lysate transfected with pCMV6-ENTRY Keap1 cDNA

    Lysates/proteins at 5 µg per lane.

    Developed using the ECL technique.

    Predicted band size: 70 kDa



    HEK293T cell lysates were generated from transient transfection of the cDNA clone (RC202189)
  • All lanes : Anti-Keap1 antibody [1B4] (ab119403) at 1/500 dilution

    Lane 1 : HepG2 cell extract
    Lane 2 : HeLa cell extract
    Lane 3 : SVT2 cell extract
    Lane 4 : A549 cell extract
    Lane 5 : COS7 cell extract
    Lane 6 : Jurkat cell extract
    Lane 7 : MDCK cell extract
    Lane 8 : PC12 cell extract
    Lane 9 : MCF7 cell extract

    Lysates/proteins at 35 µg per lane.

    Developed using the ECL technique.

    Predicted band size: 70 kDa



    HEK293T cell lysates were generated from transient transfection of the cDNA clone (RC202189)
  • ab119403 at 1/150 dilution staining Keap1 in paraffin-embedded Human endometrium tissue by Immunohistochemistry.
  • ab119403 at 1/150 dilution staining Keap1 in paraffin-embedded Human endometrium adenocarcinoma tissue by Immunohistochemistry.
  • ab119403 at 1/150 dilution staining Keap1 in paraffin-embedded Human bladder carcinoma tissue by Immunohistochemistry.
  • ab119403 at 1/100 dilution staining Keap1 in HEK293T cells transfected with either pCMV6-ENTRY Keap1 overexpress plasmid (Red) or empty vector control plasmid (Blue) and then analysed by Flow Cytometry.
  • ab119403 at 1/100 dilution staining Keap1 in HeLa cells by Flow cytometry (Red) compared to a nonspecific negative control antibody (Blue).
  • ab119403 at 1/100 dilution staining Keap1 in Jurkat cells by Flow cytometry (Red) compared to a nonspecific negative control antibody (Blue).

References

This product has been referenced in:
  • Parvez S  et al. T-REX on-demand redox targeting in live cells. Nat Protoc 11:2328-2356 (2016). Read more (PubMed: 27809314) »
  • Ziros PG  et al. Nrf2 is commonly activated in papillary thyroid carcinoma, and it controls antioxidant transcriptional responses and viability of cancer cells. J Clin Endocrinol Metab 98:E1422-7 (2013). Read more (PubMed: 23766517) »

See all 2 Publications for this product

Customer reviews and Q&As

Thank you for taking the time to contact us. I am sorry to hear you have had difficulty obtaining satisfactory results from this antibody.

I would like to reassure you that this antibody is tested and covered by our 6 month guarantee for IHC...

Read More

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

Sign up