The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use a concentration of 0.2 - 0.6 µg/ml. Detects a band of approximately 75 kDa (predicted molecular weight: 70 kDa).
Use a concentration of 5 µg/ml.
Use a concentration of 3 - 5 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Retains NFE2L2/NRF2 in the cytosol. Functions as substrate adapter protein for the E3 ubiquitin ligase complex formed by CUL3 and RBX1. Targets NFE2L2/NRF2 for ubiquitination and degradation by the proteasome, thus resulting in the suppression of its transcriptional activity and the repression of antioxidant response element-mediated detoxifying enzyme gene expression. May also retain BPTF in the cytosol. Targets PGAM5 for ubiquitination and degradation by the proteasome.
Broadly expressed, with highest levels in skeletal muscle.
ab118285 Immunofluorescence analysis of paraformaldehyde fixed HeLa cells, permeabilized with 0.15% Triton. Primary incubation 1hr (5µg/ml) followed by Alexa Fluor 488 secondary antibody (2µg/ml), showing nuclear and cytoplasmic staining. The nuclear stained with DAPI.
Western blot - Anti-Keap1 antibody (ab118285)
Anti-Keap1 antibody (ab118285) at 0.2 µg/ml + NIH3T3 cell lysate (in RIPA buffer) at 35 µg
ab118285 (3.8µg/ml) staining of paraffin embedded Human Cerebellum following steamed antigen retrieval with citrate buffer pH 6 and AP-staining shows textured cytoplasm staining in Purkinje cell and processes.