Product nameAnti-Keap1 antibody
See all Keap1 primary antibodies
DescriptionRabbit polyclonal to Keap1
Tested applicationsSuitable for: WB, ICC/IFmore details
Species reactivityReacts with: Mouse, Rat, Human
Synthetic peptide within Human Keap1 aa 574-624 (C terminal). The exact sequence is proprietary. Synthetic peptide corresponding to 19 amino acids near the C-terminus of Human Keap1.
Database link: NP_987096
- WB: Recombinant Human Keap1 protein (ab132384), Daudi, HeLa, and K562 cell lysate, and human lung tissue lysate. IHC-P: Human lung tissue.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. Stable for 12 months at -20°C.
Storage bufferPreservative: 0.02% Sodium azide
Constituent: 99% PBS
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab139729 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use a concentration of 1 - 2 µg/ml. Predicted molecular weight: 70 kDa.|
|ICC/IF||Use a concentration of 20 µg/ml.|
FunctionRetains NFE2L2/NRF2 in the cytosol. Functions as substrate adapter protein for the E3 ubiquitin ligase complex formed by CUL3 and RBX1. Targets NFE2L2/NRF2 for ubiquitination and degradation by the proteasome, thus resulting in the suppression of its transcriptional activity and the repression of antioxidant response element-mediated detoxifying enzyme gene expression. May also retain BPTF in the cytosol. Targets PGAM5 for ubiquitination and degradation by the proteasome.
Tissue specificityBroadly expressed, with highest levels in skeletal muscle.
Sequence similaritiesContains 1 BACK (BTB/Kelch associated) domain.
Contains 1 BTB (POZ) domain.
Contains 6 Kelch repeats.
DomainThe Kelch repeats mediate interaction with NF2L2/NRF2, BPTF and PGAM5.
modificationsUbiquitinated and subject to proteasomal degradation.
Cellular localizationCytoplasm. Nucleus. Shuttles between cytoplasm and nucleus.
- Information by UniProt
- Cytosolic inhibitor of Nrf2 antibody
- INrf 2 antibody
- INrf2 antibody
All lanes : Anti-Keap1 antibody (ab139729) at 2 µg/ml
Lane 1 : Wild-type HeLa cell lysate
Lane 2 : KEAP1 knockout HeLa cell lysate
Lysates/proteins at 10 µg per lane.
All lanes : HRP-conjugated goat anti-rabbit IgG at 1/10000 dilution
Predicted band size: 70 kDa
Incubated with the primary antibody for 1 hour at room temperature in 5% NFDM/TBST.
Incubated with anti-beta actin at 1µg/ml.
Immunofluorescence of Keap1 in human lung tissue with ab139727 at 20µg/mL.
Lane 1 : Anti-Keap1 antibody (ab139729) at 1 µg/ml
Lane 2 : Anti-Keap1 antibody (ab139729) at 2 µg/ml
All lanes : Human lung tissue lysate
Lysates/proteins at 15 µg per lane.
Predicted band size: 70 kDa
This product has been referenced in:
- Zhang S et al. A novel, highly-water-soluble apigenin derivative provides neuroprotection following ischemia in male rats by regulating the ERK/Nrf2/HO-1 pathway. Eur J Pharmacol 855:208-215 (2019). Read more (PubMed: 30880180) »
- Chen L et al. Unilateral ureteral obstruction causes gut microbial dysbiosis and metabolome disorders contributing to tubulointerstitial fibrosis. Exp Mol Med 51:38 (2019). Read more (PubMed: 30918245) »