Anti-Keap1 antibody (ab218815)
Key features and details
- Rabbit polyclonal to Keap1
- Suitable for: ICC/IF, IHC-P
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-Keap1 antibody
See all Keap1 primary antibodies -
Description
Rabbit polyclonal to Keap1 -
Host species
Rabbit -
Tested applications
Suitable for: ICC/IF, IHC-Pmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat, Pig, Orangutan -
Immunogen
Recombinant fragment corresponding to Human Keap1 aa 1-150.
Database link: Q14145 -
Positive control
- IHC-P: Human cerebral cortex, skeletal muscle, Fallopian tube and testis tissue. ICC: U-251 MG whole cells.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.20
Preservative: 0.02% Sodium azide
Constituents: 40% Glycerol (glycerin, glycerine), 59% PBS -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab218815 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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ICC/IF |
Use a concentration of 0.25 - 2 µg/ml.
Fixation/Permeabilization: PFA/Triton X-100 |
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IHC-P |
1/200 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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Notes |
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ICC/IF
Use a concentration of 0.25 - 2 µg/ml. Fixation/Permeabilization: PFA/Triton X-100 |
IHC-P
1/200 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
Target
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Function
Retains NFE2L2/NRF2 in the cytosol. Functions as substrate adapter protein for the E3 ubiquitin ligase complex formed by CUL3 and RBX1. Targets NFE2L2/NRF2 for ubiquitination and degradation by the proteasome, thus resulting in the suppression of its transcriptional activity and the repression of antioxidant response element-mediated detoxifying enzyme gene expression. May also retain BPTF in the cytosol. Targets PGAM5 for ubiquitination and degradation by the proteasome. -
Tissue specificity
Broadly expressed, with highest levels in skeletal muscle. -
Sequence similarities
Contains 1 BACK (BTB/Kelch associated) domain.
Contains 1 BTB (POZ) domain.
Contains 6 Kelch repeats. -
Domain
The Kelch repeats mediate interaction with NF2L2/NRF2, BPTF and PGAM5. -
Post-translational
modificationsUbiquitinated and subject to proteasomal degradation. -
Cellular localization
Cytoplasm. Nucleus. Shuttles between cytoplasm and nucleus. - Information by UniProt
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Database links
- Entrez Gene: 9817 Human
- Entrez Gene: 50868 Mouse
- Entrez Gene: 100173389 Orangutan
- Entrez Gene: 100136900 Pig
- Entrez Gene: 117519 Rat
- Omim: 606016 Human
- SwissProt: Q14145 Human
- SwissProt: Q9Z2X8 Mouse
see all -
Alternative names
- Cytosolic inhibitor of Nrf2 antibody
- INrf 2 antibody
- INrf2 antibody
see all
Images
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Immunohistochemical analysis of human cerebral cortex tissue labeling Keap1 in the cytoplasm of neurons with ab218815 at a 1/200 dilution.
Performed heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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Immunocytochemical analysis of U-251 MG (formally U-373 MG) (Human brain glioma cell line) whole cells labeling Keap1 (green) in the nucleoplasm, cytosol and microtubule organizing center with ab218815 at 2 µg/mL.
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Immunohistochemical analysis of human skeletal muscle tissue labeling Keap1 in the cytoplasm of myocytes with ab218815 at a 1/200 dilution.
Performed heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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Immunohistochemical analysis of human Fallopian tube tissue labeling Keap1 in the cytoplasm of glandular cells with ab218815 at a 1/200 dilution.
Performed heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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Immunohistochemical analysis of human testis tissue labeling Keap1 in the cytoplasm of cells in seminiferous ducts with ab218815 at a 1/200 dilution.
Performed heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (13)
ab218815 has been referenced in 13 publications.
- Zhao X et al. Antioxidant Activity of Novel Casein-Derived Peptides with Microbial Proteases as Characterized via Keap1-Nrf2 Pathway in HepG2 Cells. J Microbiol Biotechnol 31:1163-1174 (2021). PubMed: 34226415
- Jiang G et al. p62 promotes proliferation, apoptosis-resistance and invasion of prostate cancer cells through the Keap1/Nrf2/ARE axis. Oncol Rep 43:1547-1557 (2020). PubMed: 32323805
- Wang ML et al. Andrographolide sodium bisulfate attenuates UV-induced photo-damage by activating the keap1/Nrf2 pathway and downregulating the NF-?B pathway in HaCaT keratinocytes. Int J Mol Med 45:343-352 (2020). PubMed: 31789424
- Cheng Q et al. Zearalenone regulates key factors of the Keap1-Nrf2 signaling pathway in duodenum of post-weaning gilts. Asian-Australas J Anim Sci N/A:N/A (2020). PubMed: 33152220
- Zhang J et al. Illicium verum extracts and probiotics with added glucose oxidase promote antioxidant capacity through upregulating hepatic and jejunal Nrf2/Keap1 of weaned piglets. J Anim Sci 98:N/A (2020). PubMed: 32161959