Anti-Keap1 antibody - N-terminal (ab166721)

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Western blot - Anti-Keap1 antibody - N-terminal (ab166721)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Keap1 antibody - N-terminal (ab166721)

Key features and details

  • Goat polyclonal to Keap1 - N-terminal
  • Suitable for: WB, IHC-P
  • Reacts with: Mouse, Human
  • Isotype: IgG

Overview

  • Product name

    Anti-Keap1 antibody - N-terminal
    See all Keap1 primary antibodies

  • Description

    Goat polyclonal to Keap1 - N-terminal

  • Host species

    Goat

  • Specificity

    Reported variants represent identical protein: NP_036421.2 and NP_987096.1.

  • Tested applications

    Suitable for: WB, IHC-Pmore details

  • Species reactivity

    Reacts with: Mouse, Human
    Predicted to work with: Rat, Horse, Hamster, Cow, Dog, Pig, Monkey, Gorilla, Common marmoset, Orangutan

  • Immunogen

    Synthetic peptide with a Cysteine residue linker, corresponding to N terminal amino acids 41-53 of Human Keap1 (NP_036421.2).

    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI

  • Positive control

    • NIH 3T3 lysate; Human Brain, cerebellum tissue.

  • General notes

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

  • Storage buffer

    pH: 7.30
    Preservative: 0.02% Sodium azide
    Constituents: 99% Tris buffered saline, 0.5% BSA
  • Concentration information loading...

  • Purity

    Immunogen affinity purified

  • Clonality

    Polyclonal

  • Isotype

    IgG

  • Research areas

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab166721 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
Use a concentration of 0.2 - 0.6 µg/ml. Predicted molecular weight: 70 kDa.
IHC-P
Use a concentration of 3.75 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Notes
WB
Use a concentration of 0.2 - 0.6 µg/ml. Predicted molecular weight: 70 kDa.
IHC-P
Use a concentration of 3.75 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Target

  • Function

    Retains NFE2L2/NRF2 in the cytosol. Functions as substrate adapter protein for the E3 ubiquitin ligase complex formed by CUL3 and RBX1. Targets NFE2L2/NRF2 for ubiquitination and degradation by the proteasome, thus resulting in the suppression of its transcriptional activity and the repression of antioxidant response element-mediated detoxifying enzyme gene expression. May also retain BPTF in the cytosol. Targets PGAM5 for ubiquitination and degradation by the proteasome.

  • Tissue specificity

    Broadly expressed, with highest levels in skeletal muscle.

  • Sequence similarities

    Contains 1 BACK (BTB/Kelch associated) domain.
    Contains 1 BTB (POZ) domain.
    Contains 6 Kelch repeats.

  • Domain

    The Kelch repeats mediate interaction with NF2L2/NRF2, BPTF and PGAM5.

  • Post-translational
    modifications

    Ubiquitinated and subject to proteasomal degradation.

  • Cellular localization

    Cytoplasm. Nucleus. Shuttles between cytoplasm and nucleus.
  • Information by UniProt

  • Database links

    see all

  • Alternative names

    • Cytosolic inhibitor of Nrf2 antibody
    • INrf 2 antibody
    • INrf2 antibody
    • Keap 1 antibody
    • KEAP1 antibody
    • KEAP1_HUMAN antibody
    • Kelch like ECH associated protein 1 antibody
    • Kelch like family member 19 antibody
    • Kelch like protein 19 antibody
    • Kelch-like ECH-associated protein 1 antibody
    • Kelch-like protein 19 antibody
    • KIAA0132 antibody
    • KLHL 19 antibody
    • KLHL19 antibody
    • MGC10630 antibody
    • MGC1114 antibody
    • MGC20887 antibody
    • MGC4407 antibody
    • MGC9454 antibody
    see all

Images

  • Western blot - Anti-Keap1 antibody - N-terminal (ab166721)
    Western blot - Anti-Keap1 antibody - N-terminal (ab166721)
    Anti-Keap1 antibody - N-terminal (ab166721) at 0.2 µg/ml + NIH 3T3 lysate (in RIPA buffer) at 35 µg

    Developed using the ECL technique.

    Predicted band size: 70 kDa

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Keap1 antibody - N-terminal (ab166721)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Keap1 antibody - N-terminal (ab166721)
    Immunohistochemical analysis of Formalin-Fixed, Paraffin-Embedded Human Brain Cerebellum tissue labeling Keap1 with ab166721 at 3.75 µg/ml.

References (0)

Publishing research using ab166721? Please let us know so that we can cite the reference in this datasheet.

ab166721 has not yet been referenced specifically in any publications.

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