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    keap1-antibody-n-terminal-ab166721.pdf

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Epigenetics and Nuclear Signaling Chromatin Modifying Enzymes Ubiquitylation
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Anti-Keap1 antibody - N-terminal (ab166721)

  • Datasheet
  • SDS
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Western blot - Anti-Keap1 antibody - N-terminal (ab166721)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Keap1 antibody - N-terminal (ab166721)

Key features and details

  • Goat polyclonal to Keap1 - N-terminal
  • Suitable for: WB, IHC-P
  • Reacts with: Mouse, Human
  • Isotype: IgG

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Protein
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Recombinant Human Keap1 protein (Tagged) (ab132384)
Primary
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Anti-Nrf2 antibody (ab137550)
Secondary
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Donkey Anti-Goat IgG H&L (Alexa Fluor® 488) (ab150129)

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Overview

  • Product name

    Anti-Keap1 antibody - N-terminal
    See all Keap1 primary antibodies
  • Description

    Goat polyclonal to Keap1 - N-terminal
  • Host species

    Goat
  • Specificity

    Reported variants represent identical protein: NP_036421.2 and NP_987096.1.
  • Tested applications

    Suitable for: WB, IHC-Pmore details
  • Species reactivity

    Reacts with: Mouse, Human
    Predicted to work with: Rat, Horse, Hamster, Cow, Dog, Pig, Monkey, Gorilla, Common marmoset, Orangutan
  • Immunogen

    Synthetic peptide with a Cysteine residue linker, corresponding to N terminal amino acids 41-53 of Human Keap1 (NP_036421.2).

    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI
  • Positive control

    • NIH 3T3 lysate; Human Brain, cerebellum tissue.
  • General notes

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage buffer

    pH: 7.30
    Preservative: 0.02% Sodium azide
    Constituents: 99% Tris buffered saline, 0.5% BSA
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

    • Epigenetics and Nuclear Signaling
    • Chromatin Modifying Enzymes
    • Ubiquitylation

Associated products

  • Compatible Secondaries

    • Donkey Anti-Goat IgG H&L (Alexa Fluor® 488) (ab150129)
    • Donkey Anti-Goat IgG H&L (HRP) (ab205723)
  • Isotype control

    • Goat IgG, polyclonal - Isotype Control (ab37373)
  • Positive Controls

    • NIH/3T3 whole cell lysate (ab7179)
  • Recombinant Protein

    • Recombinant Human Keap1 protein (Tagged) (ab132384)
  • Related Products

    • Recombinant Human Keap1 protein (Tagged) (ab132384)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab166721 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
Use a concentration of 0.2 - 0.6 µg/ml. Predicted molecular weight: 70 kDa.
IHC-P
Use a concentration of 3.75 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Notes
WB
Use a concentration of 0.2 - 0.6 µg/ml. Predicted molecular weight: 70 kDa.
IHC-P
Use a concentration of 3.75 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Target

  • Function

    Retains NFE2L2/NRF2 in the cytosol. Functions as substrate adapter protein for the E3 ubiquitin ligase complex formed by CUL3 and RBX1. Targets NFE2L2/NRF2 for ubiquitination and degradation by the proteasome, thus resulting in the suppression of its transcriptional activity and the repression of antioxidant response element-mediated detoxifying enzyme gene expression. May also retain BPTF in the cytosol. Targets PGAM5 for ubiquitination and degradation by the proteasome.
  • Tissue specificity

    Broadly expressed, with highest levels in skeletal muscle.
  • Sequence similarities

    Contains 1 BACK (BTB/Kelch associated) domain.
    Contains 1 BTB (POZ) domain.
    Contains 6 Kelch repeats.
  • Domain

    The Kelch repeats mediate interaction with NF2L2/NRF2, BPTF and PGAM5.
  • Post-translational
    modifications

    Ubiquitinated and subject to proteasomal degradation.
  • Cellular localization

    Cytoplasm. Nucleus. Shuttles between cytoplasm and nucleus.
  • Target information above from: UniProt accession Q14145 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 532791 Cow
    • Entrez Gene: 476713 Dog
    • Entrez Gene: 9817 Human
    • Entrez Gene: 50868 Mouse
    • Entrez Gene: 100173389 Orangutan
    • Entrez Gene: 100136900 Pig
    • Entrez Gene: 117519 Rat
    • Omim: 606016 Human
    • SwissProt: Q14145 Human
    • SwissProt: Q9Z2X8 Mouse
    • SwissProt: Q5R774 Orangutan
    • SwissProt: Q684M4 Pig
    • SwissProt: P57790 Rat
    • Unigene: 465870 Human
    • Unigene: 248266 Mouse
    • Unigene: 23467 Rat
    see all
  • Alternative names

    • Cytosolic inhibitor of Nrf2 antibody
    • INrf 2 antibody
    • INrf2 antibody
    • Keap 1 antibody
    • KEAP1 antibody
    • KEAP1_HUMAN antibody
    • Kelch like ECH associated protein 1 antibody
    • Kelch like family member 19 antibody
    • Kelch like protein 19 antibody
    • Kelch-like ECH-associated protein 1 antibody
    • Kelch-like protein 19 antibody
    • KIAA0132 antibody
    • KLHL 19 antibody
    • KLHL19 antibody
    • MGC10630 antibody
    • MGC1114 antibody
    • MGC20887 antibody
    • MGC4407 antibody
    • MGC9454 antibody
    see all

Images

  • Western blot - Anti-Keap1 antibody - N-terminal (ab166721)
    Western blot - Anti-Keap1 antibody - N-terminal (ab166721)
    Anti-Keap1 antibody - N-terminal (ab166721) at 0.2 µg/ml + NIH 3T3 lysate (in RIPA buffer) at 35 µg

    Developed using the ECL technique.

    Predicted band size: 70 kDa

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Keap1 antibody - N-terminal (ab166721)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Keap1 antibody - N-terminal (ab166721)
    Immunohistochemical analysis of Formalin-Fixed, Paraffin-Embedded Human Brain Cerebellum tissue labeling Keap1 with ab166721 at 3.75 µg/ml.

Protocols

  • Western blot protocols
  • Immunohistochemistry protocols

Click here to view the general protocols

Datasheets and documents

  • SDS download

  • Datasheet download

    Download

References (0)

Publishing research using ab166721? Please let us know so that we can cite the reference in this datasheet.

ab166721 has not yet been referenced specifically in any publications.

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