Anti-Keap1 antibody - N-terminal (ab166721)
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Overview
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Product nameAnti-Keap1 antibody - N-terminal
See all Keap1 primary antibodies -
DescriptionGoat polyclonal to Keap1 - N-terminal
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Host speciesGoat
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SpecificityReported variants represent identical protein: NP_036421.2 and NP_987096.1.
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Tested applicationsSuitable for: WB, IHC-Pmore details
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Species reactivityReacts with: Mouse, Human
Predicted to work with: Rat, Horse, Hamster, Cow, Dog, Pig, Monkey, Gorilla, Common marmoset, Orangutan
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Immunogen
Synthetic peptide with a Cysteine residue linker, corresponding to N terminal amino acids 41-53 of Human Keap1 (NP_036421.2).
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Positive control
- NIH 3T3 lysate; Human Brain, cerebellum tissue.
Properties
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FormLiquid
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Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
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Storage bufferpH: 7.30
Preservative: 0.02% Sodium azide
Constituents: 99% Tris buffered saline, 0.5% BSA -
Concentration information loading... -
PurityImmunogen affinity purified
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ClonalityPolyclonal
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IsotypeIgG
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Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
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Related Products
Applications
Our Abpromise guarantee covers the use of ab166721 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | Use a concentration of 0.2 - 0.6 µg/ml. Predicted molecular weight: 70 kDa. | |
| IHC-P | Use a concentration of 3.75 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
Target
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FunctionRetains NFE2L2/NRF2 in the cytosol. Functions as substrate adapter protein for the E3 ubiquitin ligase complex formed by CUL3 and RBX1. Targets NFE2L2/NRF2 for ubiquitination and degradation by the proteasome, thus resulting in the suppression of its transcriptional activity and the repression of antioxidant response element-mediated detoxifying enzyme gene expression. May also retain BPTF in the cytosol. Targets PGAM5 for ubiquitination and degradation by the proteasome.
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Tissue specificityBroadly expressed, with highest levels in skeletal muscle.
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Sequence similaritiesContains 1 BACK (BTB/Kelch associated) domain.
Contains 1 BTB (POZ) domain.
Contains 6 Kelch repeats. -
DomainThe Kelch repeats mediate interaction with NF2L2/NRF2, BPTF and PGAM5.
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Post-translational
modificationsUbiquitinated and subject to proteasomal degradation. -
Cellular localizationCytoplasm. Nucleus. Shuttles between cytoplasm and nucleus.
- Information by UniProt
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Database links
- Entrez Gene: 532791 Cow
- Entrez Gene: 476713 Dog
- Entrez Gene: 9817 Human
- Entrez Gene: 50868 Mouse
- Entrez Gene: 100173389 Orangutan
- Entrez Gene: 100136900 Pig
- Entrez Gene: 117519 Rat
- Omim: 606016 Human
see all -
Alternative names
- Cytosolic inhibitor of Nrf2 antibody
- INrf 2 antibody
- INrf2 antibody
see all
Images
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Western blot - Anti-Keap1 antibody - N-terminal (ab166721)Anti-Keap1 antibody - N-terminal (ab166721) at 0.2 µg/ml + NIH 3T3 lysate (in RIPA buffer) at 35 µg
Developed using the ECL technique.
Predicted band size: 70 kDa -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Keap1 antibody - N-terminal (ab166721)Immunohistochemical analysis of Formalin-Fixed, Paraffin-Embedded Human Brain Cerebellum tissue labeling Keap1 with ab166721 at 3.75 µg/ml.
Datasheets and documents
References
ab166721 has not yet been referenced specifically in any publications.
