Recombinant Anti-Keratin 12/K12 antibody [EPR17882] (ab185627)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR17882] to Keratin 12/K12
- Suitable for: ICC/IF, WB, IHC-P
- Reacts with: Mouse, Rat, Human
Related conjugates and formulations
Overview
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Product name
Anti-Keratin 12/K12 antibody [EPR17882]
See all Keratin 12/K12 primary antibodies -
Description
Rabbit monoclonal [EPR17882] to Keratin 12/K12 -
Host species
Rabbit -
Tested applications
Suitable for: ICC/IF, WB, IHC-Pmore details -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- WB: Keratin 12/K12 transfected HEK-293 whole cell lysate; Mouse and rat eyeball lysates. IHC-P: Mouse and rat cornea tissues. ICC/IF: Keratin 12/K12 transfected HeLa cells.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR17882 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab185627 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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ICC/IF |
1/50.
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WB |
1/5000. Detects a band of approximately 54 kDa (predicted molecular weight: 54 kDa).
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IHC-P |
1/1000. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Tested in mouse and rat. |
Notes |
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ICC/IF
1/50. |
WB
1/5000. Detects a band of approximately 54 kDa (predicted molecular weight: 54 kDa). |
IHC-P
1/1000. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. Tested in mouse and rat. |
Target
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Function
May play a unique role in maintaining the normal corneal epithelial function. Together with KRT3, essential for the maintenance of corneal epithelium integrity. -
Tissue specificity
Cornea specific. -
Involvement in disease
Defects in KRT12 are a cause of Meesmann corneal dystrophy (MECD) [MIM:122100]; also abbreviated MCD and known as juvenile epithelial corneal dystrophy of Meesmann. MECD is an autosomal dominant disease that causes fragility of the anterior corneal epithelium. Patients are usually asymptomatic until adulthood when rupture of the corneal microcysts may cause erosions, producing clinical symptoms such as photophobia, contact lens intolerance and intermittent diminution of visual acuity. Rarely, subepithelial scarring causes irregular corneal astigmatism and permanent visual impairment. Histological examination shows a disorganized and thickened epithelium with widespread cytoplasmic vacuolation and numerous small, round, debris-laden intraepithelial cysts. -
Sequence similarities
Belongs to the intermediate filament family. - Information by UniProt
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Database links
- Entrez Gene: 3859 Human
- Entrez Gene: 268482 Mouse
- Entrez Gene: 360625 Rat
- Omim: 601687 Human
- SwissProt: Q99456 Human
- SwissProt: Q64291 Mouse
- SwissProt: Q6IFW5 Rat
- Unigene: 66739 Human
see all -
Alternative names
- CK-12 antibody
- Cytokeratin-12 antibody
- K12 antibody
see all
Images
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Immunohistochemical analysis of paraffin-embedded Mouse cornea tissue labeling Keratin 12/K12 with ab185627 at 1/100 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution. Cytoplasmic staining on the mouse corneal epithelium is observed. Counter stained with Hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.
Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
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Immunohistochemical analysis of paraffin-embedded Rat cornea tissue labeling Keratin 12/K12 with ab185627 at 1/100 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution. Cytoplasmic staining on the rat corneal epithelium is observed. Counter stained with Hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.
Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
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Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized HeLa (Human epithelial cells from cervix adenocarcinoma) cells labeling Keratin 12/K12 with ab185627 at 1/50 dilution, followed by Goat anti-rabbit IgG (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution (green). Confocal image showing no staining on HeLa cell line.
The expression increased after transfection of Keratin 12/K12. The nuclear counterstain is DAPI (blue). Tubulin is detected with ab7291 (anti-Tubulin mouse mAb) at 1/1000 dilution and ab150120 (AlexaFluor®594 Goat anti-Mouse secondary) at 1/1000 dilution (red).The negative controls are as follows:-
-ve control 1: ab185627 at 1/50 dilution followed by ab150120 (AlexaFluor®594 Goat anti-Mouse secondary) at 1/1000 dilution.
-ve control 2: ab7291 (anti-Tubulin mouse mAb) at 1/1000 dilution followed by ab150077 (Alexa Fluor®488 Goat Anti-Rabbit IgG H&L) at 1/1000 dilution.
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All lanes : Anti-Keratin 12/K12 antibody [EPR17882] (ab185627) at 1/100000 dilution
Lane 1 : Empty vector (vector control) transfected HEK-293 (Human epithelial cells from embryonic kidney) whole cell lysate
Lane 2 : Keratin 12/K12 transfected HEK-293 whole cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/50000 dilution
Predicted band size: 54 kDa
Observed band size: 54 kDa
Exposure time: 5 secondsBlocking/Dilution buffer: 5% NFDM/TBST.
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All lanes : Anti-Keratin 12/K12 antibody [EPR17882] (ab185627) at 1/5000 dilution
Lane 1 : Mouse eyeball lysate
Lane 2 : Rat eyeball lysate
Lane 3 : HeLa (Human epithelial cells from cervix adenocarcinoma) whole cell lysate
Lane 4 : Y79 (Human retinoblastoma cell line) whole cell lysate
Lane 5 : C6 (Rat glial tumor cells) whole cell lysate
Lane 6 : PC-12 (Rat adrenal gland pheochromocytoma) whole cell lysate
Lane 7 : Rat brain lysate
Lane 8 : Rat heart lysate
Lane 9 : Rat kidney lysate
Lane 10 : Rat spleen lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/50000 dilution
Predicted band size: 54 kDa
Observed band size: 54 kDa
Exposure time: 10 secondsBlocking/Dilution buffer: 5% NFDM/TBST.
Keratin 12/K12 is expressed specifically in corneal epithelium (Am J Hum Genet. 1997. 61, 1268–1275).
Protocols
Datasheets and documents
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SDS download
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Datasheet download
Certificate of Compliance
References (27)
ab185627 has been referenced in 27 publications.
- Wang M et al. The Therapeutic Roles of Recombinant Hsp90α on Cornea Epithelial Injury. Invest Ophthalmol Vis Sci 63:30 (2022). PubMed: 35201262
- Sun YC et al. Transmembrane Mucin 1 Blocks Fluorescein Ingress to Corneal Epithelium. Invest Ophthalmol Vis Sci 63:31 (2022). PubMed: 35212722
- Eriksen AZ et al. Protocols for SARS-CoV-2 infection in primary ocular cells and eye organoids. STAR Protoc 3:101383 (2022). PubMed: 35664254
- Dou S et al. Single-cell atlas of keratoconus corneas revealed aberrant transcriptional signatures and implicated mechanical stretch as a trigger for keratoconus pathogenesis. Cell Discov 8:66 (2022). PubMed: 35821117
- Puri S et al. Hyaluronan supports the limbal stem cell phenotype during ex vivo culture. Stem Cell Res Ther 13:384 (2022). PubMed: 35907870